Herbicide Diuron as Endocrine Disrupting Chemicals (EDCs) through Histopathalogical Analysis in Gonads of Javanese Medaka (Oryzias javanicus, Bleeker 1854)

Abstract

The expeditious augmentation of the agriculture industry is leaving a significant negative impact on aquatic ecosystems. However, the awareness of the impacts of herbicide Diuron toxicities on the non-targeted aquatic organism, especially fish is still lacking. Javanese medaka, a new model fish species were exposed under sublethal levels and the long-term effects on gonads were investigated via histological studies. A total of 210 sexually mature fish were exposed to Diuron at seven different concentrations; control, solvent control, 1, 50, 100, 500, and 1000 μg/L for 21 days. In this study, Diuron caused histopathological alterations in gonads (ovary and testis) of Javanese medaka (Oryzias javanicus) by decreasing in gonadal staging and maturity of germ cells in oogenesis and spermatogenesis of female and male Javanese medaka. The results obtained in this study had proven our hypothesis that long-term exposure of herbicide Diuron can cause alterations in the gonadal histology of the adults of Javanese medaka.

Embryonic toxicity of 3,4-dichloroaniline (3,4-DCA) on Javanese medaka (Oryzias javanicus Bleeker, 1854)

“…Javanese medaka ( Oryzias javanicus ) has been widely used in numerous researches as a model fish [ [22] , [23] , [24] ]. These include physical factor [ 25 ], heavy metals [ 23 , 26 ], genetics [ 27 , 27 ], fish pathology [ 25 ], organo-metals [ 28 ], and histological evidence of biocide exposure ([ 24 ], [ 29 ]). It is distributed around Peninsular Malaysia, Thailand, Singapore, and Indonesia.…”

Toxicity of Zinc Oxide Nanoparticles on the Embryo of Javanese Medaka (Oryzias javanicus Bleeker, 1854): A Comparative Study

“…Consequently, to make them spawn daily, the photoperiod was maintained at 14 h light and 10 h dark, temperature (28-30 • C), dissolved oxygen (5.5-7.5 mg/L), pH (5.5-6.5), salinity (0 ppt). Fish were fed with newly hatched Artemia nauplii (Brine shrimp) larvae, since most recent studies reported that feeding Javanese medaka with newly hatched Brine shrimp resulted in active spawning with a high number of eggs [30,33,34].…”

IV. Ikergazte. Nazioarteko ikerketa euskaraz. Kongresuko artikulu bilduma. Zientziak eta Natura Zientzia

“…Kuantifikatutako kutsatzaileak osotara lau izan dira: BPA plastifikatzaile ezaguna izan da kontzentrazio altuenean detektatutakoa (1452 ng/L), janari aditibo edota kosmetikoetan erabiltzen den butilparabenoa 37 ng/L-ko kontzentrazioan, diuron herbizida 3,2 ng/L-tan eta azkenik, progesterona sexu hormona 52 ng/L-tan. Aipatu beharra dago lau konposatu hauek disruptore endokrino bezala nabarmenduta agertzen direla bibliografian (Hashmi et al, 2020;Liu et al, 2018;Rutishauser et al, 2004;Kamarudin et al, 2020).…”