Herd-level prevalence of Mycobacterium avium subspecies paratuberculosis by bulk-tank milk PCR in Fars province (southern Iran) dairy herds

Haghkhah, Masoud; Ansari-Lari, Maryam; Novin-Baheran, Amir Mansour; Bahramy, Ayatollah

doi:10.1016/j.prevetmed.2008.03.010

Cited by 15 publications

(7 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Europe, a study from U.K. showed an apparent prevalence of 8% (Grant et al 2002), while other surveys carried out in Ireland (O'Reilly et al 2004), Denmark (Herthnek et al 2008), Switzerland (Corti and Stephan 2002), Austria (Metzger-Boddien et al 2006), Cyprus (Slana et al 2009), and Czech Republic (Slaná et al 2009) showed higher apparent prevalence values, ranging from 13% (Ireland and Denmark) to 34% (Czech Republic). Also, the variability is very high when countries outside Europe are considered, for example, Brazil (0%, Carvalho et al 2009), Iran (11%, Haghkhah et al 2008), USA (27%, Jayarao et al 2004), andMexico (100%, Favila-Humara et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia‐Romagna Region (Italy) by qPCR

et al. 2016

View full text Add to dashboard Cite

Consumption of milk and dairy products is considered one of the main routes of human exposure to Mycobacterium avium subsp. paratuberculosis (MAP). Quantitative data on MAP load in raw cows’ milk are essential starting point for exposure assessment. Our study provides this information on a regional scale, estimating the load of MAP in bulk tank milk (BTM) produced in Emilia‐Romagna region (Italy). The survey was carried out on 2934 BTM samples (88.6% of the farms herein present) using two different target sequences for qPCR (f57 and IS900). Data about the performances of both qPCRs are also reported, highlighting the superior sensitivity of IS900‐qPCR. Seven hundred and eighty‐nine samples tested MAP‐positive (apparent prevalence 26.9%) by IS900 qPCR. However, only 90 of these samples were quantifiable by qPCR. The quantifiable samples contained a median load of 32.4 MAP cells mL −1 (and maximum load of 1424 MAP cells mL −1). This study has shown that a small proportion (3.1%) of BTM samples from Emilia‐Romagna region contained MAP in excess of the limit of detection (1.5 × 101 MAP cells mL −1), indicating low potential exposure for consumers if the milk subsequently undergoes pasteurization or if it is destined to typical hard cheese production.

show abstract

Section: Discussionmentioning

confidence: 99%

Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia‐Romagna Region (Italy) by qPCR

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Bulk tank milk: In USA, Jayarao et al (2004) and Wilson et al (2010), reported 2.8-20.6% and 13.4-39.0% bio-load of MAP in bulk tank milk samples, using milk culture and IS900 milk PCR, respectively. While using milk PCR bio-load was 22.0, 49.0, 8.6-23.0 and 23.0% in UK, Chile, Iran and Switzerland, respectively (Muehlherr et al 2003;Haghkhah et al 2008;Botsaris et al 2013;Kruze et al 2013;). By using milk ELISA, the bio-load was, 9.4 and 15.4% in Chile and Denmark, respectively (Nielsen et al 2000;Salgado et al 2007).…”

Section: Map In Dairy Productsmentioning

confidence: 97%

Mycobacterium aviumsubspeciesparatuberculosis –an important food borne pathogen of high public health significance with special reference to India: an update

Chaubey

Singh

Gupta

et al. 2017

Veterinary Quarterly

View full text Add to dashboard Cite

This review underlines the public health significance of 'Indian Bison Type' of Mycobacterium avium subspecies paratuberculosis (MAP) and also its potential as 'zoonotic infection'. In the absence of control programs, bio-load of MAP is increasing and if we take total population of animals (500 million plus) and human beings (1.23 billion plus) into account, the number of infected animals and human beings will run into millions in India. Our research on screening of over 26,000 domestic livestock for MAP infection using 4 different diagnostic tests (microscopy, culture, ELISA and PCR), during last 31 years has shown that the average bio-load of MAP in the livestock population of India is very high (cattle 43%, buffaloes 36%, goats 23% and sheep 41%). 'Mass screening' of 28,291 human samples between 2008-2016 revealed also high bio-load of MAP. It has been proved that MAP is not in-activated during pasteurization and therefore live bacilli are continuously reaching human population by consumption of even pasteurized milk and other milk products. Live bacilli have also been recovered from meat products and the environment thus illustrating the potential of MAP as pathogen of public health concern. However, at present, there is inadequate scientific evidence to confirm a conclusive link between MAP infection and Johne's disease in ruminants and some cases of Crohn's disease in human beings.

show abstract

“…Mycobacterium avium subsp. paratuberculosis (MAP) was isolated in 1.6% (Goff and Griffi ths, 2006) and 8.6-23% (Haghkhah et al, 2008) of raw milk samples in the UK (Table 5.5) and Iran (Table 5.3), respectively; additionally, herd prevalences of 11% were established in Iran (Table 5.3) (Haghkhah et al, 2008). Corti and Stephan (2002) reported that 19.7% of bulk tank milk samples in Switzerland were positive for MAP (Table 5.5).…”

Section: Prevalence Of Foodborne Pathogens In Raw Milk and Milk Productsmentioning

confidence: 99%

Zoonotic pathogens in the food chain

2010

View full text Add to dashboard Cite

show abstract

Herd-level prevalence of Mycobacterium avium subspecies paratuberculosis by bulk-tank milk PCR in Fars province (southern Iran) dairy herds

Cited by 15 publications

References 24 publications

Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia‐Romagna Region (Italy) by qPCR

Estimation of Mycobacterium avium subsp. paratuberculosis load in raw bulk tank milk in Emilia‐Romagna Region (Italy) by qPCR

Mycobacterium aviumsubspeciesparatuberculosis –an important food borne pathogen of high public health significance with special reference to India: an update

Zoonotic pathogens in the food chain

Contact Info

Product

Resources

About