Hermatomyces trangensis sp. nov., a new dematiaceous hyphomycete (Hermatomycetaceae, Pleosporales) on sugar palm in Thailand

Abstract

A new species of a hyphomycetous fungus, Hermatomyces trangensis, was collected during an investigation of the diversity of palm fungi in Na Yong district, Trang province, Southern Thailand, and is introduced in this paper based on morphological and molecular evidence. The fungus is characterized by the production of only one type of conidia, globose or subglobose in front view, broadly ellipsoidal or oblong in lateral view, with dark brown to black central cells and subhyaline to pale brown peripheral cells and sporulation in culture. Phylogenetic analyses of combined SSU rDNA, LSU rDNA, ITS rDNA, TEF1 and RPB2 sequence data using maximum parsimony, maximum likelihood and Bayesian inference approaches placed the fungus within a strongly supported clade with other Hermatomyces species within the family Hermatomycetaceae (Pleosporales, Dothideomycetes). Morphologically, it is similar to other taxa with only one type of conidia such as H. sphaericus, H. sphaericoides and H. verrucosus but molecular data clearly support H. trangensis as distinct from those species.

Characterization of Nuclear and Mitochondrial Genomes of Two Tobacco Endophytic Fungi Leptosphaerulina chartarum and Curvularia trifolii and Their Contributions to Phylogenetic Implications in the Pleosporales

“…The ITS1 showed 99.73% homology with that of the L. chartarum strain with the GeneBank Accession No.KM877491.1, and the ITS4 sequence was found to possess 100% homology with that of the L. chartarum strain KNU14-16 (GenBank Accession No. KP055597.1) ( Figure 1) [23]. Our results from the phylogenetic tree analysis supported these alignment results.…”

“…For example, the phylogenomic investigation of the ITS1 sequence demonstrated that the strain was C. trifolii. In our analysis, the phylogenetic relationships based on ITS1 also showed that the L. chartarum strain had a high bootstrap value of 99% ( Figure 1) [23].…”

“…Currently, mitogenomes are commonly selected as molecular markers for phylogenetic and evolutionary analysis as they are semi-autonomous organelles with conservative gene contents and a stable gene structure [33,[43][44][45][46]. However, as mentioned previously, only a limited number of mitogenomes have been published for the Pleosporales species, even in the class Dothideomycetes [23,25,36]. Therefore, our phylogenetic tree generated from whole mitochondrial genomes serves to further the current understanding on phylogenetic relationships between Pleosporales species.…”

“…Therefore, our phylogenetic tree generated from whole mitochondrial genomes serves to further the current understanding on phylogenetic relationships between Pleosporales species. Although previous studies have successfully identified species and performed phylogenetic analysis based on single genes [4,6,8,23]. Currently, it has become increasingly common to use multiple genes to reconstruct phylogenies, as individual genes may experience different adaption pressures and, thus, produce unique substitution parameters.…”

“…Thus, it is difficult to perform classification based on their morphologies alone. Recently, single genes, including 28S and 18S were selected for inferring the phylogenetic relationships between species in the order Pleosporales [23]. Currently, the phylogenetics inferred from combined nuclear genes or organellar genomes, including plastid and mitochondrial genomes, are regarded as effective candidates for phylogenetic relationship analysis due to their moderate variation rate [24,25].…”