Herpes Simplex Antigen in Immune Complexes of Patients With Erythema Multiforme

Kazmierowski, John A.

doi:10.1001/jama.1982.03320430051031

Cited by 39 publications

(5 citation statements)

References 13 publications

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“…Erythema multiforme (minor) usually arises some days to weeks after clinically detectable herpes labialis. HSV antigens have been detected in cutaneous (152, 153) and oral lesions of EM (154) although the virus is not always observed by electromicroscopy. In situ hybridization or polymerase chain reaction (PCR) can detect HSV‐1‐DNA in the 27.2–72% of examined lesions (155–157).…”

Section: Other Diseases Associated With Hsv‐1 Infectionmentioning

confidence: 99%

Herpes Simplex Virus Type 1 infection: overview on relevant clinico‐pathological features*

Arduino

Porter

2007

J Oral Pathology Medicine

338

256

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Herpes Simplex Virus Type 1 (HSV-1) is a nuclear replicating enveloped virus, usually acquired through direct contact with infected lesions or body fluids (typically saliva). The prevalence of HSV-1 infection increases progressively from childhood, the seroprevalence being inversely related to socioeconomic background. Primary HSV-1 infections in children are either asymptomatic or following an incubation period of about 1 week gives rise to mucocutaneous vesicular eruptions. Herpetic gingivostomatitis typically affects the tongue, lips, gingival, buccal mucosa and the hard and soft palate. Most primary oro-facial HSV infection is caused by HSV-1, infection by HSV-2 is increasingly common. Recurrent infections, which occur at variable intervals, typically give rise to vesiculo-ulcerative lesions at mucocutaneous junctions particularly the lips (herpes labialis). Recurrent HSV-1 infection within the mouth is uncommon in otherwise healthy patients, although in immunocompromised patients, recurrent infection can be more extensive and/or aggressive. The diagnosis of common herpetic infection can usually be based upon the clinical history and presenting features. Confirmatory laboratory diagnosis is, however, required when patients are, or may be, immunocompromised.

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Section: Other Diseases Associated With Hsv‐1 Infectionmentioning

confidence: 99%

Herpes Simplex Virus Type 1 infection: overview on relevant clinico‐pathological features*

Arduino

Porter

2007

J Oral Pathology Medicine

338

256

View full text Add to dashboard Cite

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“…Herpes sim plex infection is associated with it [7][8][9] as is mycoplasma infection [10] as well as other infectious processes [11][12][13][14][15]. Drugs [16][17][18][19][20][21][22] 384 Nethercott/Choi are commonly associated.…”

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confidence: 99%

Erythema multiforme (Stevens-Johnson Syndrome) – Chart Review of 123 Hospitalized Patients

Nethercott

Choi

1985

Dermatology

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The charts of 123 patients hospitalized with erythema multiforme were reviewed. Antecedent infections and the administration of drugs were associated with 15 and 32% of the cases, respectively. Pyrexia arthralgia, photosensitivity, abnormal liver function tests and abnormal urine sediment were common findings. The length of stay in hospital was 12.6 ± 0.7 days (mean ± SE), time to initial improvement after onset was 10.3 ± 0.6 days and time to complete resolution was 23.0 ± 2.4 days. The presence of bullous skin lesions and greater overall severity were associated with longer hospitalization. Antecedent respiratory tract symptoms were related to shorter hospitalization. Steroid therapy was not associated with a shorter time for initial improvement to occur, while the average length of stay in hospital was 4.2 days longer than that for the patients not treated with corticosteroid.

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“…Although circulating immune complexes are thought to be present in many infectious diseases, it has been difficult to show whether the complexes are formed of antigen from the organism and specific antibody. Viral antigens have been found in circulating immune complexes or cryoprecipitates in hepatitis B (17), rubella (18), and herpes simplex infections (19), and selective concentration of specific antibody has been found in cryoprecipitates in hepatitis B (17). In addition, microbial antigens, immunoglobulin, and complement have been identified in renal biopsy specimens, in bacterial endocarditis (20,21), poststreptococcal glomerulonephritis (22), typhoid fever (23), malaria (24), and schistosomiasis (25), which has usually been interpreted as indirect evidence for the presence of microbial antigen in circulating immune complexes.…”

Section: Discussionmentioning

confidence: 99%

Evidence for pseudomonas antigen in immune complexes in pseudomonas osteomyelitis

Rahn

Steere

Grodzicki

et al. 1982

Arthritis & Rheumatism

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In infections associated with immune complex disease, microbial antigens have rarely been found in the complexes. Using an enzyme-linked immunoassay, we studied the immune complexes of a patient who had hematogenous Pseudomonas aeruginosa osteomyelitis associated with palpable purpura, arthritis, and microscopic hematuria. After separation of the complexes into high and low molecular weight fractions, a 6-fold selective concentration of P aeruginosa antibody was found in the low molecular weight fraction compared with the concentration in the serum. FolIowing disruption of immunoglobulin, the high molecular weight fraction competed with solid-phase P aeruginosa antigen for P aeruginosa antibody from another source. After successful treatment of the infection, the patient's symptoms resolved, and the complexes disappeared. These findings strongly suggest that immune complexes in this patient contained P aeruginosa antigen and antibody that may have been pathogenetic in his disease. A. HARDINAlthough circulating immune complexes are thought to be present in many diseases, it has often been difficult to identify antigen in the complexes (1). Even when immune complexes occur in infections, microbial antigens have rarely been found in the complexes (2). Furthermore, in many diseases, it remains unclear whether circulating immune complexes are pathogenetic or simply an epiphenomenon (3). We studied the immune complexes of a patient who had Pseudornonas aeruginosa vertebral osteomyelitis associated with palpable purpura, arthritis, and microscopic hematuria. We found evidence of both P aeruginosa antigen and antibody in the complexes. MATERIALS AND METHODSThe patient's blood was drawn weekly during his acute illness and less frequently (approximately monthly) during convalescence. Serum from each sample was analyzed for putative circulating immune complexes by the 1251-Clq binding assay ( 4 3 , C3 levels by radial immunodiffusion, and P aeruginosa antibody titers by indirect immunofluorescence. The isolate of P aeruginosa from the patient's blood was maintained in continuous culture on cysteine-trypticase agar.To separate the immune complexes into antigen and antibody components, 60 ml of the patient's serum, pooled from blood samples drawn early in his illness, was precipitated with polyethylene glycol as previously described (6). The precipitate (total protein 49 mg) was then dissolved in 0.lM glycine buffer, pH 3.0, and chromatographed (column dimensions: 0.9 x 60 cm) in the same buffer at 4°C on Sephacryl 5-300 (superfine) (Pharmacia Fine Chemicals, Piscataway, NJ), which had not been used in previous experiments. The eluate (total protein 37 mg) contained 2 protein peaks, one near the 19s marker (the high molecular weight fraction) and the other near the 7s marker (the low molecular weight fraction). The protein in each peak was

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Herpes Simplex Antigen in Immune Complexes of Patients With Erythema Multiforme

Cited by 39 publications

References 13 publications

Herpes Simplex Virus Type 1 infection: overview on relevant clinico‐pathological features*

Herpes Simplex Virus Type 1 infection: overview on relevant clinico‐pathological features*

Erythema multiforme (Stevens-Johnson Syndrome) – Chart Review of 123 Hospitalized Patients

Evidence for pseudomonas antigen in immune complexes in pseudomonas osteomyelitis

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