Herpes simplex virus proteins: DNA-binding proteins in infected cells and in the virus structure

Ultrastructural Localization of the Herpes Simplex Virus Major DNA-binding Protein in the Nucleus of Infected Cells

“…During the course of infection, herpes simplex virus (HSV) induces the synthesis of several DNA-binding proteins (DBPs; Bayliss et al, 1975 ;Wilcox et al, 1980). The most abundant of these is a virus-encoded polypeptide with an estimated molecular weight (Mr) of about 130000 (Bayliss et al, 1975;Leinbach & Casto, 1983).…”

DNA-binding Proteins in Frog Virus 3-infected Cells

“…Virus-specific proteins that possess high affinity for single-stranded DNA have been found in cells infected with phage T4 (Alberts & Frey, 1970), adenovirus (Van der Vliet & Levine, 1973), herpes simplex virus types 1 and 2 (Bayliss et aL, 1975;Purifoy & Powell, 1976), and vaccinia virus (Soloski et al, 1978). The best studied example of this group of proteins is gene 32 protein of phage T4.…”

“…Since then, several investigators have found DNA-binding proteins in normal cells (Salas & Green, 1971;Fox & Pardee, 1971;Tsai & Green, 1973;Choe & Rose, 1974) and in cells transformed by SV40 (Tenen et aL, 1975), and Rous sarcoma virus (Magun & Dorsett, 1977). Virus-specific DNA-binding proteins are also present in cells lytically infected with adenovirus ( Van der Vliet & Levine, 1973), herpesviruses (Bayliss et al, 1975;Purifoy & Powell, 1976), and vaccinia virus (Soloski et aL, 1978). In most cases, the function of these DNA-binding proteins has not been elucidated.…”

Identification of Two Herpes Simplex Virus Type 1-induced Proteins (21K and 22K) which Interact Specifically with the a Sequence of Herpes Simplex Virus DNA

“…Herpes simplex virus (HSV) induces at least 17 proteins which bind to DNA (Bayliss et al, 1975;Purifoy & Powell, 1976;Becker et al, 1980). Four of these have been characterized in some detail.…”

“…Most studied is the major DNA-binding protein which is essential for DNA replication (Powell et al, 1981 ;Conley et al, 1981 ;Lee & Knipe, 1983) and acts to melt the DNA helix (Powell et al, 1981). Two HSV-induced enzymes, DNA polymerase and alkaline exonuclease (Keir & Gold, 1963), also bind to DNA (Bayliss et al, 1975;Powetl & Purifoy, 1977;Hoffmann & Cheng, 1978;Strobel-Fidler & Francke, 1980;Banks et al, 1983) even when the enzymes had been purified to a degree to give a single band on SDS-polyacrylamide gels (Powell & Purifoy, 1977;Banks et al, 1983). The fourth protein, an immediate-early regulatory protein designated IE 175 or cdCP 4, does not itself bind to DNA but appears to require a cellular protein for binding (Freeman & Powell, 1982).…”