Herpes simplex virus type 2 meningitis without genital lesions: An immunoblot study

Boucquey, Donatienne; Chalon, M.P.; Sindic, Christian; Lamy, Me.; Laterre, C.

doi:10.1007/bf00314743

Cited by 14 publications

(6 citation statements)

References 21 publications

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“…The antigens used in both methods are however not specific for HSV type 1. The immunoblotting technique had already detected anti-HSV intrathecal responses in cases of menigitis and encephalitis due to HSV type 2 (Boucquey et al, 1990;Monteyne et al, 1996). The same is true for ELISAs (Ashley et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…Detection of serum and CSF anti-HSV and anti-VZV antibodies was carried out by immunoaffinity blotting [Boucquey et al, 1990;Sindic et al 1994]. CSF and sera diluted to the same IgG concentration were analysed by isoelectric focusing using agarose gels.…”

Section: Immunoaffinity-mediated Capillary Blottingmentioning

confidence: 99%

“…The second major approach is the detection of specific oligoclonal antibodies by immunoblotting. A highly sensitive antigen-mediated capillary blot technique has been developed to detect HSV-specific IgGs in CSF [Dörries et al, 1984;Boucquey et al, 1990]. It has been suggested that immunoblotting, when used in combination with PCR, provides the most accurate laboratory approach for the diagnosis of HSE [Pohl-Koppe et al, 1992], provided that samples collected after onset of antibody synthesis are examined.…”

Section: Introductionmentioning

confidence: 99%

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The detection of intrathecal synthesis of anti-herpes simplex IgG antibodies: Comparison between an antigen-mediated immunoblotting technique and antibody index calculations

et al. 1997

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The detection of intrathecal antibody synthesis was compared by the calculation of antibody indices (AI) derived from ELISA techniques with the detection of virus-specific oligoclonal IgGs by an antigen-mediated capillary blot technique. Twenty-seven paired serum and cerebrospinal fluid (CSF) samples were examined from 15 immunocompetent patients with herpes simplex virus encephalitis (HSE) diagnosed by PCR on early CSF samples. These techniques were also applied to paired samples from 20 multiple sclerosis (MS) patients, 10 patients with other inflammatory neurological diseases and 10 patients with non inflammatory neurological disorders. There was a good correlation between the results obtained by AI and those obtained by immunoblotting, especially in HSE (2 discordant results out of 27). Discrepancies were more frequent (25%) in MS patients where a "polyspecific" reaction characterized by low affinity antibodies is known to occur. Some of the discrepancies could, in part, be due to serological cross-reaction with varicella zoster virus.

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Section: Discussionmentioning

confidence: 99%

Section: Immunoaffinity-mediated Capillary Blottingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The detection of intrathecal synthesis of anti-herpes simplex IgG antibodies: Comparison between an antigen-mediated immunoblotting technique and antibody index calculations

et al. 1997

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“…[ 10 , 11 ] Infection with HSV-2 beyond the neonatal period in an otherwise healthy individual is almost always associated with a self-limited form of meningitis. [ 28 ] In contrast, HSV-1 typically causes rapidly progressive fatal necrotizing encephalitis [ 29 ] with a mortality rate of up to 70% in the absence of therapy. [ 12 , 30 ] In our study, all patients were fully recovered and discharged.…”

Section: Discussionmentioning

confidence: 99%

Viral meningitis in Sudanese children: Differentiation, etiology and review of literature

et al. 2022

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Diagnosis of viral meningitis (VM) is uncommon practice in Sudan and there is no local viral etiological map. We therefore intended to differentiate VM using standardized clinical codes and determine the involvement of herpes simplex virus types-1 and 2 (HSV-1/2), varicella zoster virus, non-polio human enteroviruses (HEVs), and human parechoviruses in meningeal infections in children in Sudan. This is a cross-sectional hospital-based study. Viral meningitis was differentiated in 503 suspected febrile attendee of Omdurman Hospital for Children following the criteria listed in the Clinical Case Definition for Aseptic/Viral Meningitis. Patients were children age 0 to 15 years. Viral nucleic acids (DNA/RNA) were extracted from cerebrospinal fluid (CSF) specimens using QIAamp® UltraSens Virus Technology. Complementary DNA was prepared from viral RNA using GoScriptTM Reverse Transcription System. Viral nucleic acids were amplified and detected using quantitative TaqMan® Real-Time and conventional polymerase chain reactions (PCRs). Hospital diagnosis of VM was assigned to 0%, when clinical codes were applied; we considered 3.2% as having VM among the total study population and as 40% among those with proven infectious meningitis. Two (0.4%) out of total 503 CSF specimens were positive for HSV-1; Ct values were 37.05 and 39.10 and virus copies were 652/PCR run (261 × 103/mL CSF) and 123/PCR run (49.3 × 103/mL CSF), respectively. Other 2 (0.4%) CSF specimens were positive for non-polio HEVs; Ct values were 37.70 and 38.30, and the approximate virus copies were 5E2/PCR run (~2E5/mL CSF) and 2E2/PCR run (~8E4/mL CSF), respectively. No genetic materials were detected for HSV-2, varicella zoster virus, and human parechoviruses. The diagnosis of VM was never assigned by the hospital despite fulfilling the clinical case definition. Virus detection rate was 10% among cases with proven infectious meningitis. Detected viruses were HSV-1 and non-polio HEVs. Positive virus PCRs in CSFs with normal cellular counts were seen.

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“…The intrathecal demonstration of oligoclonal IgG bands from MS patients by electrophoretic profiling can be used for diagnosis. However, the simultaneous increase of IgGs against different viruses may rather indicate a generalized inflammatory reponse, because infections enhance only monospecific IgGs directed against the pathogen (Boucquey et al 1990; Sindic et al 1990). In fact, binding of these antibodies to viral target structures in the CNS has not been demonstrated.…”

Section: Theories On Initiation Of Autoimmunitymentioning

confidence: 99%

Autoimmunity, dendritic cells and relevance for Parkinson’s disease

2012

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Herpes simplex virus type 2 meningitis without genital lesions: An immunoblot study

Cited by 14 publications

References 21 publications

The detection of intrathecal synthesis of anti-herpes simplex IgG antibodies: Comparison between an antigen-mediated immunoblotting technique and antibody index calculations

The detection of intrathecal synthesis of anti-herpes simplex IgG antibodies: Comparison between an antigen-mediated immunoblotting technique and antibody index calculations

Viral meningitis in Sudanese children: Differentiation, etiology and review of literature

Autoimmunity, dendritic cells and relevance for Parkinson’s disease

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