Herpes viruses in periodontal compromised sites: comparison between HIV‐positive and ‐negative patients

Grande, Sabrina Rosa; Imbronito, Ana Vitória; Okuda, Osmar Shizuo; Lotufo, Roberto Fraga Moreira; Gallottini, Marina; Nunes, Fábio Daumas

doi:10.1111/j.1600-051x.2008.01307.x

Cited by 33 publications

(54 citation statements)

References 46 publications

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“…However, the statistical analysis only showed a risk for CMV and EBV shedding in the saliva of HIV-seropositive patients. These data are consistent with other studies that showed high CMV and EBV salivary shedding among more immunosuppressed patients in comparison with HIV-seronegative individuals 4,7,10 . Even in patients submitted to HAART, EBV and CMV shedding appears to persist at a higher frequency compared with HIV-uninfected controls 8,12 .…”

Section: Ethicalsupporting

confidence: 93%

“…A 17-fold increase in the odds of triple infection detection in saliva was demonstrated. This data is consistent with other studies that reported a high prevalence of multiple HHVs in the saliva of HIV-seropositive patients and suggests that salivary shedding of all HHVs are a common event among patients receiving HAART treatment 4,10,35 . The presence of HHV coinfection in HIV-positive individuals could be due to a lack of immunosurveillance by virus-specific CD8 + cytotoxic T lymphocytes and virus specific CD4+ T cells [36][37] .…”

Section: Ethicalsupporting

confidence: 92%

“…The majority of studies report a high frequency of HHV-8 in HIV positive patients, but few studies also report high frequencies of CMV, EBV and HSV-1 and HSV-2 10,13,14 . However, there is no available data regarding the serological or molecular frequency of HHVs in the State of Piauí (PI), northeast Brazil.…”

Section: Resultsmentioning

confidence: 99%

“…The frequencies of these viruses are wide-ranging, varying from 62 to 90% for EBV; 57% for HHV-8; 19 to 72% for CMV and 5 to 16 for HSV-1 4,7,[10][11][12] . In these reports, the prevalence of HHVs was higher in HIV-seropositive patients than seronegative individuals.…”

Section: Article/artigomentioning

confidence: 99%

“…The specificities of the primer pairs were validated by DNA sequencing of the PCR products. Genomic DNAs extracted from clinical HHV-8 orf64-orf65 [20] b-globin b-globin gene [17] HSV: human herpesvirus type 1, CMV: cytomegalovirus, EBV: Epstein-Barr virus, EBNA-1: Epstein-Barr virus nuclear antigen 1 samples were used as positive control standards for HSV-1, CMV and EBV previously confirmed by PCR 10 . The genomic DNA extracted from BCBL-1 cells was used as positive control standard for HHV-8.…”

Section: Dna Extraction From Saliva Primers and Polymerase Chain Reamentioning

confidence: 99%

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PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

Carvalho

Silvestre

Maciel

et al. 2010

Rev. Soc. Bras. Med. Trop.

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Introduction: Human herpesviruses are frequently associated with orofacial diseases in humans (HSV-1, EBV, CMV and HHV-8), some can also cause systemic disease . The transmission of these viruses occurs by contact with infected secretions, especially saliva. Human immunodeficiency virus infection is associated with an increased risk of HHVs and related diseases. Methods: This work aimed to detect HSV-1, EBV, CMV and HHV-8 DNA in saliva of HIV-infected patients from Teresina, northeast Brazil, by PCR and compare these findings with age and sex matched HIV-seronegative individuals. Results: No difference in prevalence was verified between HHV detection in the saliva of HIV-seropositive individuals and controls. The individual frequencies of these viruses in these two populations were different. HIV seropositivity correlated positively with the presence of CMV (OR: 18.2, p= 0.00032) and EBV (OR: 3.44, p= 0.0081). No association between CD4 counts and the prevalence of HHVs in the saliva was observed; however, a strong association was determined between seropositivity and the presence of multiple HHV DNAs in saliva (OR: 4.83, p = 0.0028). Conclusions: These findings suggest the asymptomatic salivary shedding of HHVs is a common event between HIV-seropositive and seronegative individuals from Teresina, Piauí, Brazil, and, especially for HIV-seropositive patients, saliva is a risk factor for the acquisition/transmission of multiple HHVs. Key-words:Human herpesvirus. Saliva. PCR. HIV seropositive. RESUMOIntrodução: Alguns herpesvírus humanos são frequentemente associados a doenças orofaciais em humanos. A transmissão destes vírus ocorre através do contato com secreções contaminadas, especialmente a saliva. A infecção pelo vírus da imunodeficiência humana é considerada um fator de risco para a aquisição de HHVs e doenças correlatas. Métodos: Este trabalho teve como objetivo detectar por PCR o DNA de HSV-1, EBV, CMV e HHV-8 na saliva de pacientes infectados com HIV em Teresina, nordeste do Brasil, e comparar os dados obtidos com o grupo controle (indivíduos HIV negativos). Resultados: Não há diferença na prevalência de detecção de HHVs na saliva de indivíduos HIV soropositivos e soronegativos. No entanto, as frequências individuais de detecção dos diferentes HHVs são diferentes entre estas duas populações. A soropositividade para HIV apresentou correlação positiva com a presença de CMV (OR: 18,2, p = 0,00032) e EBV (OR: 3,44, p = 0,0081). Não foi verificada nenhuma associação entre a contagem de CD4 e prevalência de HHVs na saliva, no entanto existe uma forte associação entre a soropositividade e a detecção do DNA de vários HHVs na saliva (OR: 4,83, p = 0,0028). Conclusões: Estes resultados sugerem que a transmissão salivar de HHVs é um evento comum entre os indivíduos HIV soropositivos e soronegativos de Teresina, Piauí, Brasil, e, especialmente para os pacientes soropositivos, a saliva é um fator de risco para a aquisição/transmissão de múltiplos HHVs.Palavras-chaves: Herpesvirus humanos. Saliva. PCR. HIV sorop...

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Section: Ethicalsupporting

confidence: 93%

Section: Ethicalsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Article/artigomentioning

confidence: 99%

Section: Dna Extraction From Saliva Primers and Polymerase Chain Reamentioning

confidence: 99%

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PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

Carvalho

Silvestre

Maciel

et al. 2010

Rev. Soc. Bras. Med. Trop.

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A correlation analysis of HHV infection and its predictive factors in an HIV‐seropositive population in Yunnan, China

Ren

Wang

Miao

et al. 2019

Journal of Medical Virology

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Human herpesviruses (HHVs) have a particularly high prevalence in certain high-risk populations and cause increased morbidity and mortality in patients with acquired immunodeficiency syndrome (AIDS). Screening and treating subclinical HHV infections reduce human immunodeficiency virus (HIV) infection incidence, disease progression, and transmission. However, there are few studies on HHVs, HIV coinfection rates, and their related risk factors. We aimed to clarify the prevalence of all eight HHVs in peripheral blood samples collected from HIV-positive patients, and explore the association of HHV infection in HIV-positive patients in an HIVseropositive population in Yunnan. We recruited 121 HIV-positive patients with highly active antiretroviral therapy (HAART) and 45 healthy individuals. All the eight HHVs were detected using polymerase chain reaction and their epidemiological information and clinical data were collected and statistically analyzed. A high prevalence of HHVs (89.3%) was observed in individuals with HIV infections and with herpes simplex virus (HSV)-2 (65.3%), and HSV-1 (59.5%) being the most common.Coinfection with more than two different HHVs was more common in patients with HIV infections receiving HAART (72.7%) than in healthy controls. Older age, being married, higher HIV-1 plasma viral loads, and use of antiviral protease inhibitors were independently correlated with an increased frequency of HHVs, but we found no association with CD4 count, WHO HIV clinical stage, and HIV infection duration. Our findings are of great significance for the prevention of HHV opportunistic infection in patients with AIDS and their clinical treatment.

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Periodontal Microbiology

Dumitrescu¹,

Ohara²

2010

Etiology and Pathogenesis of Periodontal Disease

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Herpes viruses in periodontal compromised sites: comparison between HIV‐positive and ‐negative patients

Abstract: EBV-1 was more frequently recovered in oral sites of HIV-positive patients than in HIV-negative patients.

Cited by 33 publications

References 46 publications

PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

A correlation analysis of HHV infection and its predictive factors in an HIV‐seropositive population in Yunnan, China

Periodontal Microbiology

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