Herpesvirus in Bovines: Importance of Bovine Herpesvirus Type 1

Kaur, Gurpreet; Chandra, Mudit

doi:10.5772/63157

Cited by 4 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, vaccination cannot completely prevent the disease. Since BoHV-1 is a latent infection and highly contagious virus, vaccination is usually recommended at 4-6 months of age after decreased passive immunity in calves (Kaur 2016).…”

Section: Introductionmentioning

confidence: 99%

Molecular Investigation of Bovine Viral Diarrhea Virus, Bovine Herpes Virus-1 and Bovine Herpes Virus-4 Infections in Abortion Cases of Cattle in Van District, Turkey

YILDIZ

Babaoğlu

2022

Van Veterinary Journal

View full text Add to dashboard Cite

Abortions, fetal mummification, calf anomalies, and infertility problems are the majority of reproductive problems in cattle. Viruses play a significant role in the cause of these cases. In cattle, these agents are known as primary abortion agents and the most common of these agents are Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus Type 1 (BoHV-1), and Bovine Herpes Virus Type 4 (BoHV-4) infections. The objective of this research is to determine the possible role of BVDV, BoHV-1, and BoHV-4 as viral abortion agents in cattle housed in the Van district. For this, a total of 115 animal specimens (blood, serum, vaginal swab, vaginal fluid discharge, nasal swab, and abortion material) from 100 abortion, early embryonic deaths, and fertility problems cases in cattle over the age of 2-5 years old were collected. All samples for detection of BVDV, BoHV-1 and BoHV-4 genomes were tested by the Polymerase Chain Reaction (PCR) technique using specific primers encoding Panpesti 5'-UTR, Glycoprotein C (gC), and Glycoprotein B (gB) genes, respectively. Result out of the samples tested, %41.73 were positive for BVDV and all samples were negative for BoHV-1 and BoHV-4. In conclusion, the presence of BVDV in cattle in the Van region and its role in the occurrence of abortion cases was emphasized for the first time; consideration of viral abortions and determining the etiology of abortion cases and genital system problems needs to focus on the detection of persistent infected (PI) animals and the most effective way of vaccination of susceptible populations for prevention and control of infection.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular Investigation of Bovine Viral Diarrhea Virus, Bovine Herpes Virus-1 and Bovine Herpes Virus-4 Infections in Abortion Cases of Cattle in Van District, Turkey

YILDIZ

Babaoğlu

2022

Van Veterinary Journal

View full text Add to dashboard Cite

show abstract

“…BoHV-1 is an enveloped DNA virus and was selected for this study as it is of economic importance both to Ireland and internationally [27][28][29] . In addition, this virus has a well characterised and predictable pattern of infection.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of rapid untargeted nanopore DNA virus metagenomics using cell cultures and calves experimentally infected with bovine herpes virus-1

Esnault

Earley

Cormican

et al. 2021

Preprint

View full text Add to dashboard Cite

Bovine respiratory disease (BRD) is the leading cause of morbidity and mortality in cattle in Ireland, and internationally. This disease is caused by many well-known, and an ever-increasing number of newly associated viruses and bacteria. Consequently, diagnosis of BRD pathogens by targeted real-time polymerase chain reaction (qPCR) diagnostics is too expensive and slow to enable a same-day response that is targeted at the causative pathogen(s). To address this, we developed a same-day, sample to result, untargeted metagenomic MinION sequencing protocol for the identification of DNA viruses associated with BRD from nasal swabs. The procedure comprises non-viral nucleic acid depletion, nucleic acid extraction, rapid transposase-based tagmentation with barcoded adapters, non-biased PCR amplification of tagmented nucleic acid, sequencing on a MinION device, then rapid analysis of resulting sequences on cloud-based software EPI2ME WIMP. The protocol was developed using BoHV1-infected foetal lung cell cultures where we achieved 96% enrichment of the BoHV-1 sequence. Subsequently, the protocol was successfully applied to untargeted detection of BoHV-1 in nasal swabs from calves experimentally challenged with BoHV-1.

show abstract

“…These glycoproteins are major targets of immune cells. The gB, gC, gD and gE envelope glycoproteins were reported to be most abundant in the viral tegument layer (Kaur and Chandra 2016). Therefore, these glycoproteins were chosen to develop subunit vaccines or diagnostic reagents (for instance, the gB protein was used by IDEXX).…”

Section: Introductionmentioning

confidence: 99%

Polystyrene nanoparticles induce stronger cellular and more durable humoral immune responses against an inactivated bovine herpesvirus 1 isolate

Liu

Xin

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

The inactivated bovine herpesvirus type 1(BoHV-1) vaccines are generally safe and suitable for use in dairy and pregnant cattle, but induces weaker cellular immune responses and shorter antibody responses compared with the modified live virus vaccine. In this study, we used polystyrene (PS) nanoparticles (100 nm) as a carrier for purified inactivated broken BoHV-1 to improve cellular and humoral immune responses compared with the traditional inactivated vaccine. Mice were injected intramuscularly with the inactivated complex mixed with ISA206 adjuvant. Transmission electron microscopy showed that the PS nanoparticles displayed broken BoHV-1 on their surfaces. After validation of BoHV-1 and gB gC gD gE tegument proteins, it proved that the BoHV-conjugated PS nanoparticles induced higher-titer and more durable antibody responses. The inactivated BoHV-PS nanoparticle complex elicited neutralizing antibodies (titer ~2 6 ) in 5 weeks post-immunization in mice. The CD4/CD8 ratio was higher in mice immunized with PS nanoparticles compared with other groups. However, this ratio reached its maximum 1 week later than in mice immunized with ISA206+BoHV-1 or BoHV-1. Levels of interleukin (IL)-4, IL-6, and interferon-γ in followed similar patterns. In conclusion, this pilot study demonstrated that PS nanoparticles can adjuvant inactivated BoHV-1 vaccines, enhancing both cell-mediated immune responses and the duration of antibody responses. This study provides the foundation for a new development platform for inactivated vaccines, which can elicit potent cellular and humoral immune responses in animals and humans.The inactivated bovine herpesvirus type 1(BoHV-1) vaccines are generally safe and suitable for use in dairy and pregnant cattle, but induces weaker cellular immune responses and shorter antibody responses compared with the modified live virus vaccine. In this study, we used polystyrene (PS) nanoparticles (100 nm) as a carrier for purified inactivated broken BoHV-1 to improve cellular and humoral immune responses compared with the traditional inactivated vaccine. Mice were injected intramuscularly with the inactivated complex mixed with ISA206 adjuvant. Transmission electron microscopy showed that the PS nanoparticles displayed broken BoHV-1 on their surfaces. After validation of BoHV-1 and gB gC gD gE tegument proteins, it proved that the BoHV-conjugated PS nanoparticles induced higher-titer and more durable antibody responses. The inactivated BoHV-PS nanoparticle complex elicited neutralizing antibodies (titer ~2 6 ) in 5 weeks post-immunization in mice. The CD4/CD8 ratio was higher in mice immunized with PS nanoparticles compared with other groups. However, this ratio reached its maximum 1 week later than in mice immunized with ISA206+BoHV-1 or BoHV-1. Levels of interleukin (IL)-4, IL-6, and interferon-γ in followed similar patterns. In conclusion, this pilot study demonstrated that PS nanoparticles can adjuvant inactivated BoHV-1 vaccines, enhancing both cell-mediated immune responses and the duration of antibody responses. This study provides the foundation for a new development platform for inactivated vaccines, which can elicit potent cellular and humoral immune responses in animals and humans.

show abstract

Herpesvirus in Bovines: Importance of Bovine Herpesvirus Type 1

Cited by 4 publications

References 42 publications

Molecular Investigation of Bovine Viral Diarrhea Virus, Bovine Herpes Virus-1 and Bovine Herpes Virus-4 Infections in Abortion Cases of Cattle in Van District, Turkey

Molecular Investigation of Bovine Viral Diarrhea Virus, Bovine Herpes Virus-1 and Bovine Herpes Virus-4 Infections in Abortion Cases of Cattle in Van District, Turkey

Optimisation of rapid untargeted nanopore DNA virus metagenomics using cell cultures and calves experimentally infected with bovine herpes virus-1

Polystyrene nanoparticles induce stronger cellular and more durable humoral immune responses against an inactivated bovine herpesvirus 1 isolate

Contact Info

Product

Resources

About