Heshouwu (Polygonum multiflorum Thunb.) Extract Attenuates Bone Loss in Diabetic Mice

Abstract

This study investigated the effects and mechanism of Heshouwu (Polygonum multiflorum Thunb.) water extract (HSW) on diabetes-related bone loss in mice. HSW was orally administered (300 mg/kg body weight) to high-fat diet and streptozotocin-induced diabetic mice for 10 weeks. HSW significantly alleviated mouse body weight loss and hyperglycemia compared with the control group, and elevated serum levels of insulin, osteocalcin, and bone-alkaline phosphatase. HSW supplementation also significantly increased the bone volume/tissue volume ratio and trabecular thickness and number, and decreased the bone surface/bone volume ratio and trabecular structure model index in the femur and tibia. Moreover, HSW significantly increased femoral bone mineral density. In addition, HSW down-regulated osteoclastogenic genes, such as nuclear factor of activated T-cells, cytoplasmic 1 and tartrate-resistant acid phosphatase 5 (TRAP), in both the femur and tibia tissue, and reduced serum TRAP level compare to those of control mice. These results indicate that HSW might relieve diabetes-related bone disorders through regulating osteoclast-related genes, suggesting HSW may be used as a preventive agent for diabetes-induced bone loss.

Systemic elucidate the potential bioactive compounds and hypoglycemic mechanism of Polygonum multiflorum based on network pharmacology

“…Active compound and related targets What was more, ranking the target degrees, 28 targets from the average degree (3.97) to the maximum degree (15) were listed in table 3. Protein-protein interaction network…”

Systemic elucidation on the potential bioactive compounds and hypoglycemic mechanism of Polygonum multiflorum based on network pharmacology

“…(PM), a classical herbal medicine, has been used for several centuries for its functions of hair-promoted, liver-tonifying and anti-aging [ 13 , 14 ]. Previous reports have shown that extracts of PM and its compounds had hypoglycemic effects [ 15 – 17 ]. However, researches on the hypoglycemic properties of PM are still limited and scarce.…”

Systemic elucidation on the potential bioactive compounds and hypoglycemic mechanism of Polygonum multiflorum based on network pharmacology

“…(PM), a classical herbal medicine, has been used for several centuries for its functions of hair-promoted, liver-tonifying and anti-aging [13,14]. Previous reports have shown that extracts of PM and its compounds had hypoglycemic effects [15][16][17]. However, researches on the hypoglycemic properties of PM are still limited and scarce.…”

“…According to the degree of the compounds, the compounds from 2 times of the average degree(17.24) to the maximum degree(43) were screened out, namely resveratrol, apigenin, kaempferol, quercetin and luteolin, respectively, as shown in gure 2(b). What was more, ranking the target degrees, 28 targets from the average degree (3.97) to the maximum degree(15) were listed in table 3.…”