Hesperetin as an inhibitor of the snake venom serine protease from Bothrops jararaca

Santos, Roney Vander Dos; Grillo, Giovanna Rivabem; Fonseca, Henrique M.A.C.; Stanisić, Danijela; Tasić, Ljubica

doi:10.1016/j.toxicon.2021.04.016

Cited by 8 publications

(5 citation statements)

References 49 publications

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“…in reaching medical assistance, accident severity, snake species and size, snake geographic distribution, and amount of venom inoculated [8,[11][12][13][50][51][52]. The main mechanism attributed to inducing AKI involves inflammation, formation of immune complexes, hemodynamic disorders, and direct action of venom on kidney tissues [13,14,18,24,[53][54][55].…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings

Brasileiro-Martins,

Cavalcante,

Nascimento

et al. 2024

PLoS Negl Trop Dis

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Acute kidney injury (AKI) is a critical systemic complication caused by Bothrops envenoming, a neglected health problem in the Brazilian Amazon. Understanding the underlying mechanisms leading to AKI is crucial for effectively mitigating the burden of this complication. This study aimed to characterize the urinary protein profile of Bothrops atrox snakebite victims who developed AKI. We analyzed three groups of samples collected on admission: healthy subjects (controls, n = 10), snakebite victims who developed AKI (AKI, n = 10), and those who did not evolve to AKI (No-AKI, n = 10). Using liquid-chromatography tandem mass spectrometry, we identified and quantified (label-free) 1190 proteins. A panel of 65 proteins was identified exclusively in the urine of snakebite victims, with 32 exclusives to the AKI condition. Proteins more abundant or exclusive in AKI’s urine were associated with acute phase response, endopeptidase inhibition, complement cascade, and inflammation. Notable proteins include serotransferrin, SERPINA-1, alpha-1B-glycoprotein, and NHL repeat-containing protein 3. Furthermore, evaluating previously reported biomarkers candidates for AKI and renal injury, we found retinol-binding protein, beta-2-microglobulin, cystatin-C, and hepcidin to be significant in cases of AKI induced by Bothrops envenoming. This work sheds light on physiological disturbances caused by Bothrops envenoming, highlighting potential biological processes contributing to AKI. Such insights may aid in better understanding and managing this life-threatening complication.

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Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings

Brasileiro-Martins,

Cavalcante,

Nascimento

et al. 2024

PLoS Negl Trop Dis

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“…This aspect is particularly valuable when high-resolution structural data is unavailable. Furthermore, even when such information exists (a subject that has gained strength over recent years with the advancement of artificial intelligence-based methods such as AlphaFold and RoseTTAFold providing highly accurate predicted models [22,23]), CD provides crucial additional insights into the impact of various factors, such as temperature [24,25], pH [14,16,26], charge [27], ligands [28][29][30][31][32][33][34], protein/peptide binding [35,36], mutations [33,37,38] and others on protein secondary structure. This is because CD is a sensitive technique that can detect subtle changes in protein conformation, even when these changes are not large enough to be observed in high-resolution structural data.…”

Section: Further Advantages Of CD Spectroscopy In Protein Researchmentioning

confidence: 99%

Unlocking Insights into Folding, Structure, and Function of Proteins through Circular Dichroism Spectroscopy—A Short Review

Linhares,

Ramos

2023

Applied Biosciences

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Circular dichroism (CD) spectroscopy has emerged as a powerful tool in the study of protein folding, structure, and function. This review explores the versatile applications of CD spectroscopy in unraveling the intricate relationship between protein conformation and biological activity. A key advantage of CD spectroscopy is its ability to analyze protein samples with minimal quantity requirements, making it an attractive technique for studying proteins that are scarce or difficult to produce. Moreover, CD spectroscopy enables the monitoring of physical and chemical environmental effects on protein structures, providing valuable insights into the dynamic behavior of proteins in different conditions. In recent years, the use of synchrotron radiation as a light source for CD measurements has gained traction, offering enhanced sensitivity and resolution. By combining the advantages of CD spectroscopy, such as minimal sample requirements and the ability to probe environmental effects, with the emerging capabilities of synchrotron radiation (SRCD), researchers have an unprecedented opportunity to explore the diverse aspects of protein behavior. This review highlights the significance of CD spectroscopy in protein research and the growing role of synchrotron radiation in advancing our understanding of protein behavior, aiming to provide novel insights and applications in various fields, including drug discovery, protein engineering, and biotechnology. A brief overview of Solid-State Circular Dichroism (SSCD) is also included.

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“…They are functionally similar to thrombin as they can cleave the fibrinogen, causing coagulopathy and hypofibrinogenemia [47,50]. Inhibitors of snake venom serine proteases have been identified, including metabolites hesperetin (plant flavonoid) [51], synthetic peptides pepB (Pro-Phe-D-Arg-Gln-Ala-Ser) and pepC (Pro-Phe-Tyr-Gln-Ser-Ser) [52] that inhibited SVSP from Bothrops jararaca. These SVSP inhibitors can be used to improve envenomation treatment.…”

Section: Snake Venom Serine Proteasesmentioning

confidence: 99%

A Review of the Proteomic Profiling of African Viperidae and Elapidae Snake Venoms and Their Antivenom Neutralisation

Offor

Muller²,

Piater

2022

Toxins

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Snakebite envenoming is a neglected tropical disease (NTD) that results from the injection of snake venom of a venomous snake into animals and humans. In Africa (mainly in sub-Saharan Africa), over 100,000 envenomings and over 10,000 deaths per annum from snakebite have been reported. Difficulties in snakebite prevention and antivenom treatment are believed to result from a lack of epidemiological data and underestimated figures on snakebite envenoming-related morbidity and mortality. There are species- and genus-specific variations associated with snake venoms in Africa and across the globe. These variations contribute massively to diverse differences in venom toxicity and pathogenicity that can undermine the efficacy of adopted antivenom therapies used in the treatment of snakebite envenoming. There is a need to profile all snake venom proteins of medically important venomous snakes endemic to Africa. This is anticipated to help in the development of safer and more effective antivenoms for the treatment of snakebite envenoming within the continent. In this review, the proteomes of 34 snake venoms from the most medically important snakes in Africa, namely the Viperidae and Elipdae, were extracted from the literature. The toxin families were grouped into dominant, secondary, minor, and others based on the abundance of the protein families in the venom proteomes. The Viperidae venom proteome was dominated by snake venom metalloproteinases (SVMPs–41%), snake venom serine proteases (SVSPs–16%), and phospholipase A2 (PLA2–17%) protein families, while three-finger toxins (3FTxs–66%) and PLA2s (16%) dominated those of the Elapidae. We further review the neutralisation of these snake venoms by selected antivenoms widely used within the African continent. The profiling of African snake venom proteomes will aid in the development of effective antivenom against snakebite envenoming and, additionally, could possibly reveal therapeutic applications of snake venom proteins.

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Hesperetin as an inhibitor of the snake venom serine protease from Bothrops jararaca

Cited by 8 publications

References 49 publications

Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings

Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings

Unlocking Insights into Folding, Structure, and Function of Proteins through Circular Dichroism Spectroscopy—A Short Review

A Review of the Proteomic Profiling of African Viperidae and Elapidae Snake Venoms and Their Antivenom Neutralisation

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