Hesperetin loaded proposomal gel for topical antioxidant activity

Vaz, Vanessa Marcia; Jitta, Srinivas Reddy; Verma, Ruchi; Kumar, Lalit

doi:10.1016/j.jddst.2021.102873

Cited by 14 publications

(31 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, BDM nanoparticles showed a lower IC 50 than free BDM ethanolic solution, with BDM-loaded PLHNs antioxidant activity improving by 11%, aligning with results using CUR that showed antioxidant activity improved by 17% [ 78 ]. In sum, our results show that the antioxidant activity of DMC and BDM was significantly enhanced by the PLHNs preparation, consistent with previous results on polymeric nanoparticle formulations of curcuminoid mixtures [ 79 ] and the individual curcuminoids DMC and BDM [ 59 ], aligning also with results obtained for other polyphenols, such as hesperetin formulations [ 80 ].…”

Section: Resultssupporting

confidence: 92%

Design of Hybrid Polymeric-Lipid Nanoparticles Using Curcumin as a Model: Preparation, Characterization, and In Vitro Evaluation of Demethoxycurcumin and Bisdemethoxycurcumin-Loaded Nanoparticles

et al. 2021

View full text Add to dashboard Cite

Polymeric lipid hybrid nanoparticles (PLHNs) are the new generation of drug delivery systems that has emerged as a combination of a polymeric core and lipid shell. We designed and optimized a simple method for the preparation of Pluronic F-127-based PLHNs able to load separately demethoxycurcumin (DMC) and bisdemethoycurcumin (BDM). CUR was used as a model compound due to its greater availability from turmeric and its structure similarity with DMC and BDM. The developed method produced DMC and BDM-loaded PLHNs with a size average of 75.55 ± 0.51 and 15.13 ± 0.014 nm for DMC and BDM, respectively. An FT-IR analysis confirmed the encapsulation and TEM images showed their spherical shape. Both formulations achieved an encapsulation efficiency ≥ 92% and an exhibited significantly increased release from the PLHN compared with free compounds in water. The antioxidant activity was enhanced as well, in agreement with the improvement in water dissolution; obtaining IC50 values of 12.74 ± 0.09 and 16.03 ± 0.55 for DMC and BDM-loaded PLHNs, respectively, while free curcuminoids exhibited considerably lower antioxidant values in an aqueous solution. Hence, the optimized PHLN synthesis method using CUR as a model and then successfully applied to obtain DMC and BDM-loaded PLHNs can be extended to curcuminoids and molecules with a similar backbone structure to improve their bioactivities.

show abstract

Section: Resultssupporting

confidence: 92%

Design of Hybrid Polymeric-Lipid Nanoparticles Using Curcumin as a Model: Preparation, Characterization, and In Vitro Evaluation of Demethoxycurcumin and Bisdemethoxycurcumin-Loaded Nanoparticles

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The percentage EE of the proposomes was estimated by using the dissolution process. [40][41][42] The unentrapped naringin was estimated in PB with pH 7.4. The proposomal formulation was packed in a dialysis membrane.…”

Section: Percentage Entrapment Efficiency (Ee)mentioning

confidence: 99%

“…After 1 h, 1 ml of the buffer was taken from the receiver compartment, and the absorbance was measured at 283 nm using UV-Vis spectrophotometer (UV-1601PC, Shimadzu, Corporation). 42 Percentage EE = Total drug amount − free drug amount Total drug amount × 100 TA B L E 1 Variables and their levels used for the optimization of proposomes S. no Factors…”

Section: Percentage Entrapment Efficiency (Ee)mentioning

confidence: 99%

“…Then, further the mechanism of drug release from the prepared formulation was confirmed with Korsmeyer peppas plot. 42,44…”

Section: In Vitro Drug Release Kineticsmentioning

confidence: 99%

“…The sample preparation and experiment were performed in a dark to avoid the degradation. 42 Methanol was used as a blank. The percent inhibition was calculated by using the following equation 42 :…”

Section: Antioxidant Activitymentioning

confidence: 99%

See 2 more Smart Citations

Design and development of naringin‐loaded proposomal gel for wound healing

Kumari

Chevala

Jitta

et al. 2022

J of Cosmetic Dermatology

View full text Add to dashboard Cite

Background:The injuries or wounds caused by various means will impact human lives severely. An increase in the demand for wounds or burns was observed. For better wound healing and to combat the free radical effect on the healing process, wounds must be treated with multifunctional or multipurpose dressing or gel or any other type of biomaterial. Objectives:The study aimed to develop, optimize, and evaluate the naringin-loaded proposomal gel (PPG) for quick wound healing. Methods:The central composite design was employed for the optimization of proposomes. Naringin-loaded proposomes were evaluated for percentage entrapment efficiency (EE), the particle size of proposomes (PsP), and the zeta potential of proposomes (ZpP). The change in drug release profile was studied by dissolution.Furthermore, naringin and naringin-loaded proposomes, antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) reagent and ascorbic acid as a reference standard. Different gel bases were prepared, and based on various parameters, the G2 (0.6% Carbopol 974) gel base was selected for naringin proposomes loading. The naringin-loaded PPG was evaluated for various in vitro and in vivo wound-healing properties. Results:The optimized naringin-loaded proposomes showed extended drug release (90.78 ± 2.19%) for 72 h. The naringin-loaded PPG improved the permeability of naringin, which showed 28.91 ± 2.81% of drug release after 96 h, and the drug solution showed 9.05 ± 0.92%. IC50 values of antioxidant activity of naringin and naringin proposomes were found to be 337.31 μg/ml and 201.86 μg/ml, respectively. The naringin-loaded PPG showed better wound closure on the 15th day (3.32%) compared with proposomal solution (4.75%) or naringin topical gel (4.2%). Conclusion:Based on the obtained results, we conclude naringin-loaded PPG can be an alternative strategic approach to deliver the naringin for quick wound healing.

show abstract