Hesperetin Modifies the Composition of Fecal Microbiota and Increases Cecal Levels of Short-Chain Fatty Acids in Rats

Unno, Toshihiro; Hisada, Takayoshi; Takahashi, Shunsuke

doi:10.1021/acs.jafc.5b02649

Cited by 61 publications

(43 citation statements)

References 34 publications

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“…After centrifugation, the supernatant was passed through disposable solid‐phase extraction column cartridges (Strata‐X, 30 mg/ml, Phenomenex, Inc., Torrance, CA, USA), and 0.4 ml of the passed eluate was added to the same volume of acetonitrile. After centrifugation at 5,000 g for 10 min, liberated maltose in the supernatant was measured by the HPLC system (Unno et al., ). The rate of enzyme inhibition (%) was calculated as maltose concentration in the resulting mixture relative to the control (without GTE or BTE).…”

Section: Methodsmentioning

confidence: 99%

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Green tea extract and black tea extract differentially influence cecal levels of short‐chain fatty acids in rats

Unno

Osakabe

2018

Food Science & Nutrition

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Increasing evidence indicates that gut microbiota plays a critical role to maintain the host's health. The biological function of microbially produced short‐chain fatty acids (SCFA) becomes the focus of attention. This study aimed to compare the effects of green tea extract (GTE) and black tea extract (BTE) on cecal levels of SCFA in rats. Rats consumed an assigned diet of either a control diet, a GTE diet (10 g/kg), or a BTE diet (10 g/kg), for 3 weeks. The dietary addition of GTE significantly reduced the concentrations of acetate and butyrate in cecal digesta compared to the control, but BTE showed an increased trend for a cecal pool. In the GTE group, a significant amount of undigested starch was excreted in feces, but BTE produced no effect. Interestingly, feces of rats fed the BTE diet contained higher bacterial 16S rRNA gene copy numbers for total eubacteria compared to the control diet. Taken together, treatments of the diets with GTE and BTE brought about a different degree of producing SCFA in rat cecum. BTE might advantageously stimulate more SCFA production than GTE by facilitating bacterial utilization of starch.

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Section: Methodsmentioning

confidence: 99%

“…After centrifugation at 5,000 g for 10 min, liberated maltose in the supernatant was measured by the HPLC system (Unno et al, 2015). The rate of enzyme inhibition (%) was calculated as maltose concentration in the resulting mixture relative to the control (without GTE or BTE).…”

Section: Inhibition Of Pancreatic α-Amylase In Vitromentioning

confidence: 99%

Green tea extract and black tea extract differentially influence cecal levels of short‐chain fatty acids in rats

Unno

Osakabe

2018

Food Science & Nutrition

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“…Dietary supplements, or nutraceuticals, have been used extensively as a natural treatment for CVD and related diseases as they exhibit antioxidant, anti‐inflammatory, or hypotensive effects . In particular, hesperidin, a flavanone glycoside that is found abundantly in citrus fruits, is metabolized by the gut microbiota to its aglycone form, hesperetin , which is known to have cardioprotective effects , and some studies already demonstrated its hypotensive effects in spontaneously hypertensive rats (SHR) . Roowi et al.…”

Section: Introductionmentioning

confidence: 99%

Multi‐omics approach to elucidate the gut microbiota activity: Metaproteomics and metagenomics connection

et al. 2018

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Over the last few years, the application of high-throughput meta-omics methods has provided great progress in improving the knowledge of the gut ecosystem and linking its biodiversity to host health conditions, offering complementary support to classical microbiology. Gut microbiota plays a crucial role in relevant diseases such as obesity or cardiovascular disease (CVD), and its regulation is closely influenced by several factors, such as dietary composition. In fact, polyphenol-rich diets are the most palatable treatment to prevent hypertension associated with CVD, although the polyphenol-microbiota interactions have not been completely elucidated. For this reason, the aim of this study was to evaluate microbiota effect in obese rats supplemented by hesperidin, after being fed with cafeteria or standard diet, using a multi meta-omics approaches combining strategy of metagenomics and metaproteomics analysis. We reported that cafeteria diet induces obesity, resulting in changes in the microbiota composition, which are related to functional alterations at proteome level. In addition, hesperidin supplementation alters microbiota diversity and also proteins involved in important metabolic pathways. Overall, going deeper into strategies to integrate omics sciences is necessary to understand the complex relationships between the host, gut microbiota, and diet.

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“…Bacteria associated with fermented foods may influence gut-associated microbial composition and function by direct competition, by metabolic interaction, through direct immune activation, or via the production of bioactive molecules, such as the short-chain fatty acids (SCFAs), that are able to influence the host health regulating a number of immune and non-immune protective mechanisms (9)(10)(11)(12)(13).…”

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confidence: 99%

Specific Signatures of the Gut Microbiota and Increased Levels of Butyrate in Children Treated with Fermented Cow's Milk Containing Heat-Killed Lactobacillus paracasei CBA L74

Canani

Filippis

Nocerino

et al. 2017

Appl Environ Microbiol

108

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We recently demonstrated that cow's milk fermented with the probiotic CBA L74 (FM-CBAL74) reduces the incidence of respiratory and gastrointestinal tract infections in young children attending school. This effect apparently derives from a complex regulation of non-immune and immune protective mechanisms. We investigated whether FM-CBAL74 could regulate gut microbiota composition and butyrate production. We randomly selected 20 healthy children (12 to 48 months) from the previous randomized controlled trial, before (t0) and after 3 months (t3) of dietary treatment with FM-CBAL74 (FM) or placebo (PL). Fecal microbiota was profiled using 16S rRNA gene amplicon sequencing, and the fecal butyrate concentration was also measured. Microbial alpha and beta diversities were not significantly different between groups prior to treatment. FM-CBAL74 but not PL treatment increased the relative abundance of Individual ,, and oligotypes were associated with FM-CBAL74 treatment and demonstrated correlative associations with immune biomarkers. Accordingly, PICRUSt analysis predicted an increase in the proportion of genes involved in butyrate production pathways, consistent with an increase in fecal butyrate observed only in the FM group. Dietary supplementation with FM-CBAL74 induces specific signatures in gut microbiota composition and stimulates butyrate production. These effects are associated with changes in innate and acquired immunity. The use of a fermented milk product containing the heat-killed probiotic strain CBAL74 induces changes in the gut microbiota, promoting the development of butyrate producers. These changes in the gut microbiota composition correlate with increased levels of innate and acquired immunity biomarkers.

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Hesperetin Modifies the Composition of Fecal Microbiota and Increases Cecal Levels of Short-Chain Fatty Acids in Rats

Cited by 61 publications

References 34 publications

Green tea extract and black tea extract differentially influence cecal levels of short‐chain fatty acids in rats

Green tea extract and black tea extract differentially influence cecal levels of short‐chain fatty acids in rats

Multi‐omics approach to elucidate the gut microbiota activity: Metaproteomics and metagenomics connection

Specific Signatures of the Gut Microbiota and Increased Levels of Butyrate in Children Treated with Fermented Cow's Milk Containing Heat-Killed Lactobacillus paracasei CBA L74

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