Hesperetin treatment attenuates glycation of lens proteins and advanced‑glycation end products generation

Abstract

advanced glycation end products (aGes) in lens proteins increase with aging, thus inducing cataracts and/or presbyopia. Hesperetin (Hst), which is an abundant plant flavanone largely derived from citrus species, and its derivatives attenuate cataracts and presbyopia in vivo and in vitro; however, no reports have described its effects on aGe formation in lens proteins. The present study demonstrated that aGes in lens proteins increase with age in mice. additionally, it showed that Hst can prevent aGes and n(ε)-carboxymethyl-lysine generation and modification of lens proteins using in vitro in human lens epithelial cell lines and ex vivo in mouse lens organ cultures. Furthermore, treatment with Hst prevented lens hardening and decreased chaperone activity in lens proteins. These results suggested that Hst and its derivatives are good candidates for the prevention of presbyopia and cataracts.

Computational and Experimental Approaches Exploring the Role of Hesperetin in Improving Autophagy in Rat Diabetic Retinopathy

“…Hesperetin is an inexpensive flavanone with strong antioxidant activity that is mainly obtained from citrus species [ 52 ]. It is also known to have anti-inflammatory and COX2-inhibitory action [ 53 ], neuroprotective activity [ 54 ] and to decrease vascular permeability [ 55 ].…”

“…They documented that hesperetin was able to upregulate the antioxidative mechanisms contributing to human SH-SY5Y neuroblastoma cells against AGE-induced ROS [ 87 ]. Similarly, Doki et al found that therapeutic doses of hesperetin attenuated glycation of lens proteins and AGE generation [ 52 ]. Furthermore, Kara et al reported that hesperetin provided protection from apoptosis in a rat ischemia–reperfusion retinal injury model [ 88 ].…”

Hesperetin activates CISD2 to attenuate senescence in human keratinocytes from an older person and rejuvenates naturally aged skin in mice

“…In addition, we found that hesperetin influences advanced glycation end products (AGE) and the receptor for the AGE (RAGE) pathway, which have been reported to be abnormally increased in several tissues during aging [ 65 , 66 ]. Interestingly, studies from other groups have suggested that hesperetin is able to attenuate AGE generation and inhibit AGE-induced oxidative stress [ 67 , 68 ]. The RAGE pathway, involving AGEs and their receptor RAGE, plays a significant role in the aging process, particularly in the context of skin health.…”

A Cataract-Causing Mutation in the TRPM3 Cation Channel Disrupts Calcium Dynamics in the Lens

“…Further, they suggest that TRPM3 channels may participate in the non-selective cation leak long associated with lens aging and cataract formation. The fact that TRPM3 channels can be inhibited by certain drugs (e.g., NSAIDs) and nutraceuticals-including citrus flavanones that have been associated with the attenuation of cataract [93,94]-raises the possibility of therapeutic interventions for TRPM3 cataract.…”