Hesperidin from Citrus sinensis Cultivated in Dezful, Iran

Abstract

This study described procedures for extracting and quantitation of hesperidin in the waste orange peel of Dezful. Two extracting procedure were used. In procedure A hesperidin was isolated from orange peel by extracting the dry peel first with petroleum ether, removing the essential oil and then with methanol. In procedure B alkaline extraction followed by acidification of the extract was used. It was purified by treatment with formamide-activated charcoal. Detailed analysis of UV, IR, 1HNMR, 13CNMR and Mass spectroscopic data confirm the structure and extent of purity of extracted hesperidin. The spectroscopic results of two extract showed that procedure A produced high extraction yield and more purified hesperidin. Pure hesperidin in gram quantity (11.7% for procedure A and 7.39% for procedure B) was obtained in one purification cycle.

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“…Its deficiency has been associated with pain in extremities. Supplemental hesperidin also helps to reduce edema or extreme swelling of the legs due to fluid accumulation (Aghel et al, 2008).…”

The impact of some natural phenolic compounds on carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and α‐glycosidase enzymes: An antidiabetic, anticholinergic, and antiepileptic study

“…Hesperidin compound is a plant flavanone that abundantly and predominantly exists in citrus fruits. Also, most flavonoid compounds are bound to a sugar part and are named the glycoside molecules . Zingerone is an inexpensive and nontoxic compound with diverse pharmacological activities.…”

“…Also, most flavonoid compounds are bound to a sugar part and are named the glycoside molecules. [15] Zingerone is an inexpensive and nontoxic compound with diverse pharmacological activities. Zingerone, a biologically active phenolic alkanone found in Zingiber officinale, had various pharmacological activities including anti-apoptotic, antiinflammatory, and protecting myocardial infarction and irritable bowel disorder.…”

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“…All the CC fractions were subjected to enzyme assay and the most active fraction on preparative TLC yielded compounds 1–3 , using the following systems: compound 1 (15 mg), chloroform–ethyl acetate (7:3); compound 2 (17 mg), ethyl acetate–methanol (9:1); compound 3 (13 mg), ethyl acetate–methanol (9.8:0.2), as shown in Fig. . The chloroform‐soluble fraction (CSC) of Camellia sinensis (1.73 g) was chromatographed using a silica gel column, which afforded CC fractions SA‐1, SA‐2, SA‐3, SA‐4 and SA‐5 on elution with n ‐hexane (100%, 250 mL), n ‐hexane–EtOAc (9:10, 250 mL), n ‐hexane–EtOAc (70:30, 250 mL), n ‐hexane–EtOAc (20:80, 200 mL) and EtOAc (100%, 250 mL), respectively.…”

“…1. 21,22 The chloroform-soluble fraction (CSC) of Camellia sinensis (1.73 g) was chromatographed using a silica gel column, which afforded CC fractions SA-1, SA-2, SA-3, SA-4 and SA-5 on elution with n-hexane (100%, 250 mL), n-hexane-EtOAc (9:10, 250 mL), nhexane-EtOAc (70:30, 250 mL), n-hexane-EtOAc (20:80, 200 mL) and EtOAc (100%, 250 mL), respectively. Preparative TLC of the n-hexane-EtOAc fraction in the system of n-hexane-ethyl acetate (10:90) resulted in compound 4 (10 mg), as shown in Fig.…”