Hesperidin modulates the rhythmic proteomic profiling in Drosophila melanogaster under oxidative stress

Abstract

The circadian clock regulates vital aspects of physiology including protein synthesis and oxidative stress response. In this investigation, we performed a proteome-wide scrutiny of rhythmic protein accrual in Drosophila melanogaster on exposure to rotenone, rotenone + hesperidin and hesperidin in D. melanogaster. Total protein from fly samples collected at 6 h intervals over the 24 h period was subjected to two-dimensional gel electrophoresis and mass spectrometry. Bioinformatics tool, Protein ANalysis THrough Evolutionary Relationships classification system was used to the determine the biological processes of the proteins of altered abundance. Conspicuous variations in the proteome (151 proteins) of the flies exposed to oxidative stress (by rotenone treatment) and after alleviating oxidative stress (by hesperidin treatment) were observed during the 24 h cycle. Significantly altered levels of abundance of a wide variety of proteins under oxidative stress (rotenone treatment) and under alleviation of oxidative stress (rotenone + hesperidin treatment) and hesperidin (alone) treatment were observed. These proteins are involved in metabolism, muscle activity, heat shock response, redox homeostasis, protein synthesis/folding/degradation, development, ion-channel/cellular transport, and gustatory and olfactory function of the flies. Our data indicates that

Deficiency of circadian gene Per2 blocks luteolin-induced adipocyte browning in mice through weakening liver PPARα/RXRα/FGF21 pathway

“…These findings highlight an association between circadian rhythms and adipocyte browning, which provides a new perspective for treating metabolic diseases, such as obesity and its complications. Several flavonoids have been found to affect the circadian rhythm [37] , such as nobiletin enhances the circadian rhythm amplitude and protects against metabolic syndrome in diet-induced obese mice [38] . LU as one of the most common flavonoids, can ameliorate obesity and associated comorbidity via regulating the interplay between the liver and adipose tissue [17] and enhancing adipocyte browning in mice [18] .…”

Metabolic Basis of Circadian Dysfunction in Parkinson’s Disease

“…Additionally, there was a decrease in the expression of the molecular clock target genes nuclear receptor Rev-ErbA α (Nr1d1) and D-box-binding protein (Dbp) [79]. The expression levels of guanine nucleotide-binding protein subunit, lactate dehydrogenase, enolase, fructose bisphosphate, glycogen phosphorylase, and phosphoglycerate kinase vary in response to rotenone treatment [80]. These results are corroborated by a study by Mattam et al, who measured changes in the rhythmic dynamic equilibrium of interactions between various elements of serotonin metabolism and the molecular clock in the rotenone-induced PD male Wistar rat model.…”

“…In addition, the mechanism of action of SR8278 focused in particular on the crosstalk and genome-wide motif enrichment of REV-ERBA α and NURR1, another transcription factor implicated in the regulation of dopamine signaling [77]. Dietary addition of hesperidin, one of the antioxidant compounds in vegetables and fruits, controls the levels of the ROS, prevents DNA damage and neuro-inflammation, and restores rhythmicity [80]. Several studies [102,103] have indicated that dopaminergic treatment may induce a phase advance in the melatonin rhythm.…”