Hesperidin reduces dentin wear after erosion and erosion/abrasion cycling in vitro

Leal, Isabelly de Carvalho; Rabelo, Cibele Sales; Viana, Ítallo Emídio Lira; Scaramucci, Taís; Santiago, Sérgio Lima; Passos, Vanara Florêncio

doi:10.1016/j.archoralbio.2021.105208

Cited by 9 publications

(9 citation statements)

References 39 publications

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“…The research protocol, specifically the regimen of erosive challenges, the application times, and the concentrations of the tested inhibitors were determined according to previous studies. 5 , 14 , 16 , 23 In addition, among all known CCs, catK is the only one with triple helical collagenase activity 14 and it has been indicated that pro-MMP-9 can be activated by catK. 13 Therefore, we used ODN in this study because it is a specific inhibitor of catK.…”

Section: Discussionmentioning

confidence: 99%

“…Before the erosive challenges, specimens were first soaked in artificial saliva (containing 0.4 g/l NaCl; 0.795 g/l CaCl 2 ·H 2 O; 0.4 g/l KCl; 0.005 g/l Na 2 S·9H 2 O; 0.69 g/l NaH 2 PO 4 ·H 2 O; 0.3 g/l KSCN; and 1 g/l urea, pH=6.8) at 37°C for 1 h. 16 In the subgroup PRE, the specimens were immersed in 50 ml of the respective solutions for 5 min, followed by a 10 s rinse with deionized water. 23 Subsequently, the specimens were immersed in 150 ml of a cola drink (pH=2.3, Coca Cola, Fuzhou, China) for 5 min and rinsed with deionized water for 10 s; 23 the cycle was conducted four times (predetermined times at 8, 12, 16, 20 h) per day 24 for 5 d. Conversely, the specimens in the subgroup POST were first placed in 150 ml of a cola drink for 5 min and then immersed in 50 ml of the different solutions for 5 min. Similarly, a 10 s deionized water rinse was conducted when the acid challenges and solution treatments were finished.…”

Section: Methodsmentioning

confidence: 99%

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Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion?

et al. 2023

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Objectives To evaluate the effects of matrix metalloproteinase (MMP) and cathepsin K (catK) inhibitors on resistance to dentin erosion. Methodology A total of 96 dentin specimens (3×3×2 mm) were prepared and randomly assigned into four groups (n=24): deionized water (DW); 1 µM odanacatib (ODN, catK inhibitor); 1 mM 1,10-phenanthroline (PHEN, MMP inhibitor); and 1 µM odanacatib + 1 mM 1,10-phenanthroline (COM). Each group was further divided into two subgroups for the application of treatment solutions before (PRE) and after erosive challenges (POST). All specimens were subjected to four daily erosive challenges for 5 d. For each erosive challenge, the specimens in subgroup PRE were immersed in the respective solutions before cola drinks, while the specimens in subgroup POST were immersed in the respective solutions after cola drinks (the immersion duration was 5 min in both cases). All specimens were stored in artificial saliva at 37°C between erosive challenges. The erosive dentin loss (EDL) was measured by profilometry. The residual demineralized organic matrix (DOM) of specimens was removed using type VII collagenase and evaluated by profilometry. Both the EDL and thickness of the residual DOM were statistically analyzed by two-way analysis of variance (ANOVA) and Bonferroni’s test (α=0.05). The surface topography and transverse sections of the specimens were observed using SEM. MMPs and catK were immunolabeled in the eroded dentin and in situ zymography was performed to evaluate the enzyme activity. Results Significantly lower EDL was found in the groups ODN, PHEN, and COM than in the control group (all p<0.05), while no significant difference in EDL was found among the groups ODN, PHEN, and COM (all p>0.05). The application sequence showed no significant effect on the EDL of the tested groups (p=0.310). A significantly thicker DOM was observed in the group ODN than in the control group regardless of the application sequence (both p<0.05). The treatment with ODN, PHEN, and COM inhibited the gelatinolytic activity by approximately 46.32%, 58.6%, and 74.56%, respectively. Conclusions The inhibition of endogenous dentinal MMPs and catK increases the acid resistance of human dentin but without an apparent synergistic effect. The inhibition of MMPs and catK is equally effective either before or after the acid challenge.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion?

et al. 2023

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“…Dentin erosion is further modulated by a demineralized organic matrix (DOM), which may reduce the progression of erosive lesions [Kato et al, 2012;Martines De Souza et al, 2017]. Since DOM is susceptible to cleavage by matrix metalloproteinases (MMPs) and cysteine proteases, inhibiting the activity of those proteases to slow the progression of dentin erosion has attracted increasing attention from researchers and clinicians [Montagner et al, 2014;Seseogullari-Dirihan et al, 2017;Altinci et al, 2018;Leal et al, 2021]. Chlorhexidine (CHX) has been reported to reduce the dentin organic matrix and mineral losses due to erosion by inhibiting the activity of MMPs [Zarella et al, 2015;Boteon et al, 2017].…”

Section: Introductionmentioning

confidence: 99%

“…Chlorhexidine (CHX) has been reported to reduce the dentin organic matrix and mineral losses due to erosion by inhibiting the activity of MMPs [Zarella et al, 2015;Boteon et al, 2017]. Flavonoids, a main group of natural bioactive MMP inhibitors, reduced erosive dentin loss (EDL) by 20-70% and increased the surface microhardness by 10-30% compared with deionized water [de Moraes et al, 2020;Jiang et al, 2020;Leal et al, 2021;Niemeyer et al, 2021].…”

Section: Introductionmentioning

confidence: 99%

Effects of Cathepsin K Inhibitors on Dentin Erosion: An in vitro Study

Yiying

Lin

et al. 2023

Caries Res

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Cathepsin K (catK) modulates the degradation of dentin collagen. This study aimed to evaluate the effects of catK inhibitors on dentin erosion. Dentin beams were eroded (4 times/d for 5 d) and immersed in deionized water (negative control), 0.1 M NaCl, 0.3 M NaCl, 0.5 M NaCl, or 1 μM odanacatib (each n = 16) for 30 min after each erosive challenge. Erosive dentin loss (EDL) and demineralized organic matrix (DOM) thickness were evaluated profilometrically. Additionally, dentin beams were demineralized, immersed in the respective solutions for 30 min each (n=5), and then incubated in artificial saliva for 5 d. Dentin collage degradation was evaluated by quantifying the levels of the C-terminal peptide of type I collagen (CTX), C-terminal cross-linked telopeptide of type I collagen (ICTP), and hydroxyproline (HYP) in the incubation media. Significantly lower EDL and dentin collagen degradation (CTX, ICTP, and HYP), and thicker DOM layers were observed in the samples treated with 0.3 M NaCl and 1 μM odanacatib than in those treated with deionized water (all P < 0.05). The samples treated with 1 μM odanacatib showed significantly lower levels of CTX and HYP than those treated with 0.3 M NaCl (all P < 0.05). The present findings support the potential use of catK inhibitors in controlling dentin erosion.

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“…However, dentin organic matrix is susceptible to degradation by MMP mainly including MMP-2, MMP-8, and MMP-9 8 . This degradation occurs because MMP are inactive in saliva and dentin, but in an acidic environment, MMP are activated and initiate their proteolytic action, thus contributing to the progression of erosive wear 9 .…”

Section: Introductionmentioning

confidence: 99%

The roles of theaflavins in reducing dentin erosion

Guo

Yang

2023

Sci Rep

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This study aimed to evaluate the effect of theaflavins [TFs] on the process of dentin erosion and investigation the potential mechanism. For erosion kinetics of the dentin, 7 experimental groups (n = 5) treated with 10% ethanol [EtOH] (negative control) are erosion for 1, 2, 3, 4, 5, 6, and 7 d erosion cycles (4 cycles/d). For the effect of TFs on dentin erosion, 6 experimental groups (n = 5) were treated with 1% epigallocatechin gallate [EGCG], 1% chlorhexidine [CHX], 1%, 2%, 4%, and 8% TFs for the 30 s and then subjected to erosion cycles (4 cycles/d for 7 d). The erosive dentin wear (μm) and surface morphology were evaluated and compared by laser scanning confocal microscope and scanning electron microscopy. The matrix metalloproteinase inhibition effects of TFs were investigated using in situ zymography and molecular docking. TFs-treated collagen was investigated by ultimate microtensile strength, Fourier-transform infrared spectroscopy, and molecular docking. Data were analyzed by ANOVA, Tukey’s test (P < 0.05). The TFs-treated groups (7.56 ± 0.39, 5.29 ± 0.61, 3.28 ± 0.33, and 2.62 ± 0.99 μm for 1%, 2%, 4%, and 8% TFs) had significantly lower erosive dentin wear than the negative control group (11.23 ± 0.82 μm), and the effect was concentration-dependent at low concentrations (P < 0.05). TFs inhibit matrix metalloproteinase [MMP]. Moreover, TFs crosslink dentin collagen and cause hydrophilic changes in dentin collagen. TFs preserve organic matrix within the demineralized dentin by inhibiting MMP activity and simultaneously improving collagen’s resistance to enzymes, both of which contribute to preventing or slowing down the progression of dentin erosion.

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Hesperidin reduces dentin wear after erosion and erosion/abrasion cycling in vitro

Cited by 9 publications

References 39 publications

Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion?

Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion?

Effects of Cathepsin K Inhibitors on Dentin Erosion: An in vitro Study

The roles of theaflavins in reducing dentin erosion

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