Hetergeneous tumour response to photodynamic therapy assessed by in vivo localised 31P NMR spectroscopy

Ceckler, Toni L.; Gibson, Scott L.; Kennedy, Scott D.; Hill, Richard A.; Bryant, Robert G.

doi:10.1038/bjc.1991.201

Cited by 12 publications

(9 citation statements)

References 26 publications

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“…It is supposed that, for many types of tumors, response to irradiation or chemotherapy appears to show the reversal of the trends seen in untreated tumor progression. Some other therapeutic modalities, notably hyperthermia ) and photodynamic therapy (Ceckler et al 1991), however, show the opposite response : acidic shift, decrease of NTP • PCr and increase of Pi • PME levels. It is suggested that those changes in 31P-MRS following irradiation or chemotherapy are due to the metabolic activation in tumor tissue caused by reoxygenation (Gohda et al 1990; Kimura et al 1992) or due to decrease in demand for high energy phosphate in the damaged cells following treatment, as the cellular energy supply is sufficient for the metabolic needs of the tumor (Steen 1989(Steen , 1991.…”

Section: Discussionmentioning

confidence: 99%

Changes of 31P-MR Spectroscopy in Experimental Tumors Following Radiotherapy Compined with 5-Fluorouracil Compound(UFT).

Ohkubo

1993

Tohoku J. Exp. Med.

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Section: Discussionmentioning

confidence: 99%

Changes of 31P-MR Spectroscopy in Experimental Tumors Following Radiotherapy Compined with 5-Fluorouracil Compound(UFT).

Ohkubo

1993

Tohoku J. Exp. Med.

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“…The pH of the R3230AC tumour is approximately 7.15, as determined by Ceckler et al (1991) using 31 P NMR spectroscopy. The Hill coefficient, n and the p 50 corresponding to this pH are 2.46 and 33.7 torr, respectively (Zwart et al, 1982(Zwart et al, , 1984.…”

Section: Figurementioning

confidence: 99%

“…Within each of these subpopulations, there is a trend toward a larger carbogen-induced change for tumours with lower initial saturation. Using a pH of 7.15 for the R3230AC tumour, which has been determined by Ceckler et al (1991) from 31 P NMR spectroscopy measurements, and the Hill parameters n (2.46) and p 50 (33.7 torr) corresponding to this pH (Zwart et al, 1982(Zwart et al, , 1984, we fit an expression of the form to the data in Figure 7 to find the average change in blood pO 2 (∆pO 2 ) induced by carbogen. The derivation of this equation is presented in the Appendix.…”

Section: Figurementioning

confidence: 99%

Carbogen-induced changes in rat mammary tumour oxygenation reported by near infrared spectroscopy

1999

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SummaryWe have evaluated the ability of steady-state, radially-resolved, broad-band near infrared diffuse reflectance spectroscopy to measure carbogen-induced changes in haemoglobin oxygen saturation (SO 2 ) and total haemoglobin concentration in a rat R3230 mammary adenocarcinoma model in vivo. Detectable shifts toward higher saturations were evident in all tumours (n = 16) immediately after the onset of carbogen breathing. The SO 2 reached a new equilibrium within 1 min and remained approximately constant during 200-300 s of administration. The return to baseline saturation was more gradual when carbogen delivery was stopped. The degree to which carbogen increased SO 2 was variable among tumours, with a tendency for tumours with lower initial SO 2 to exhibit larger changes. Tumour haemoglobin concentrations at the time of peak enhancement were also variable. In the majority of cases, haemoglobin concentration decreased in response to carbogen, indicating that increased tumour blood volume was not responsible for the observed elevation in SO 2 . We observed no apparent relationship between the extent of the change in tumour haemoglobin concentration and the magnitude of the change in the saturation. Near infrared diffuse reflectance spectroscopy provides a rapid, non-invasive means of monitoring spatially averaged changes in tumour haemoglobin oxygen saturation induced by oxygen modifiers.Keywords: oxygen; tumour oxygenation; carbogen; haemoglobin; diffuse reflectance spectroscopy; radiotherapy 1709British Journal of Cancer (1999) 79(11/12), 1709-1716 © 1999 Cancer Research Campaign Article no. bjoc.1998 Received 21 April 1998 Revised 19 August 1998 Accepted 21 August 1998 Correspondence to: TH Foster (HbO 2 ) saturation may be determined from the tissue absorption spectrum, which may be reconstructed from appropriate reflectance measurements. Successful spectroscopic determination of the HbO 2 saturation (SO 2 ) in the near infrared spectral region depends upon two things: (1) the ability to determine accurately the absorption spectrum in the presence of significant light scattering by tissue and (2) the ability to account properly for the contributions of chromophores other than haemoglobin in the measured tissue absorption spectrum. We have recently reported on a multi-wavelength, continuous-wave diffuse reflectance spectrometer and data reduction scheme that address both of these criteria Nichols et al, 1997; Hull et al, 1998). Here, we describe the use of this spectroscopic method to obtain non-invasively the response of subcutaneous rat mammary adenocarcinomas to carbogen breathing. The technique is simultaneously sensitive to both the HbO 2 saturation and to the tumour haemoglobin concentration. MATERIALS AND METHODS Near infrared spectroscopyOur instrumentation and method of data reduction for performing quantitative diffuse reflectance spectroscopy in highly scattering systems has been described in detail elsewhere (Nichols et al, 1997; Hull et al, 1998). Briefly, we have constructed a continuou...

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“…Gadolinium diethylenetriamene pentaacetate (DTPA) contrast-enhanced MRI was used to assess the boundary of PDT-induced tissue necrosis in a canine model [23] and in human patients [24]. Second, in vivo 31 P nuclear magnetic resonance (NMR) spectroscopy has been used to monitor tumor metabolic status before and after the treatment of RIF-1 tumors [25,26] and mammary carcinoma [27][28][29][30]. NMR data analysis revealed significant differences in the time course of changes in high energy phosphate levels in response to combined hyperthermia and photodynamic therapies [29].…”

Section: Introductionmentioning

confidence: 99%

High‐field magnetic resonance imaging of the response of human prostate cancer to Pc 4‐based photodynamic therapy in an animal model

et al. 2007

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Introduction-High-field magnetic resonance imaging (MRI) is an emerging technique that provides a powerful, non-invasive tool for in vivo studies of cancer therapy in animal models. Photodynamic therapy (PDT) is a relatively new treatment modality for prostate cancer, the second leading cause of cancer mortality in American males. The goal of this study was to evaluate the response of human prostate tumor cells growing as xenografts in athymic nude mice to Pc 4-sensitized PDT.

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Hetergeneous tumour response to photodynamic therapy assessed by in vivo localised 31P NMR spectroscopy

Abstract: We report here initial results using localised in vivo 31P

Cited by 12 publications

References 26 publications

Changes of 31P-MR Spectroscopy in Experimental Tumors Following Radiotherapy Compined with 5-Fluorouracil Compound(UFT).

Changes of 31P-MR Spectroscopy in Experimental Tumors Following Radiotherapy Compined with 5-Fluorouracil Compound(UFT).

Carbogen-induced changes in rat mammary tumour oxygenation reported by near infrared spectroscopy

High‐field magnetic resonance imaging of the response of human prostate cancer to Pc 4‐based photodynamic therapy in an animal model

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