Heterochromatin Distribution and Chromosomal Mapping of Microsatellite Repeats in the Genome ofFrieseomelittaStingless Bees (Hymenoptera: Apidae: Meliponini)

Santos, Jádilla Mendes dos; Diniz, Débora; Rodrigues, Tecavita Ananda Santos; Cioff, Marcelo de Bello; Waldschmidt, Ana Maria

doi:10.1653/024.101.0107

Cited by 23 publications

(16 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the chromatin composition, only 1 microsatellite was found in euchromatic regions. Generally, microsatellites have been found in euchromatic regions of insect chromosomes, for example, in locusts [Ruiz-Ruano et al, 2015], bees [Piccoli et al, 2018;Santos et al, 2018;Travenzoli et al, 2019], and ants [Barros et al, 2018]. However, our results show that most of the microsatellites analyzed are located in heterochromatic regions in P. fastidiosuscula, suggesting that they can be found in the entire chromatin depending on the species [Milani and Cabral-de-Mello, 2014].…”

Section: Discussioncontrasting

confidence: 54%

“…A eukaryote genome generally has numerous regions of repetitive DNA [Britten and Kohne, 1968] which are classified into dispersed repetitive DNA (transposons and retrotransposons) and tandem repetitive DNA (satellite DNAs, microsatellites, minisatellites, and multigene families such as rDNA) [reviewed in López-Flores and Garrido-Ramos, 2012]. Repetitive DNAs, such as microsatellites and ribosomal genes, are used as molecular markers and allow for investigating the composition and organization of chromatin, as well as chromosomal evolution in various organisms, including insects [Butcher et al, 2000;Brito et al, 2005;Lopes et al, 2014;Menezes et al, 2014;Cunha et al, 2018;Piccoli et al, 2018;Santos et al, 2018;Travenzoli et al, 2019].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First Record of a B Chromosome in Polybia fastidiosuscula Saussure (Vespidae) and Investigation of Chromatin Composition Through Microsatellite Mapping

Marchioro

Campos

Lopes

2020

Cytogenet Genome Res

View full text Add to dashboard Cite

The characterization of karyotypes is an important aspect in understanding the structure and evolution of genomes. Polybia is a genus of social wasps of the family Vespidae. This genus has 58 species, but for only 8 of these chromosome number and morphology have been reported in the literature. The aim of this study was to describe and characterize the Polybia fastidiosuscula Saussure karyotype, presenting the first case of a B chromosome in Vespidae. In addition, we investigated the chromatin composition of this species through C-banding, base-specific fluorochrome staining, and physical mapping of 7 microsatellites and 18S rDNA. Four colonies of P. fastidiosuscula from Minas Gerais and Paraná states, Brazil, were analyzed. The chromosome number identified was 2n = 34, and 2 colonies presented a B chromosome. We characterized the chromatin composition of this species, analyzing the existence of different microsatellite-rich heterochromatic regions which are also enriched with AT or GC base pairs. We suggest an intraspecific origin of the B chromosome based on the homology of the heterochromatic composition with A chromosomes and also verify that the TTAGG and TCAGG sequences are not telomeric, but only microsatellites that occur in the centromeres of most chromosomes, as well as GAG and CGG.

show abstract

Section: Discussioncontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

First Record of a B Chromosome in Polybia fastidiosuscula Saussure (Vespidae) and Investigation of Chromatin Composition Through Microsatellite Mapping

Marchioro

Campos

Lopes

2020

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…Although the exact mechanism by which 45S rDNA interacts with the genome and performs genome rearrangements is not yet fully understood, microhomology between sub-repeats in the 45S rDNA intergenic spacer and microsatellites may drive recombination, particularly given that microsatellites are known hotspots for recombination and chromosomal rearrangements [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Subgenome Discrimination in Brassica and Raphanus Allopolyploids Using Microsatellites

Campomayor

Waminal

Kang

et al. 2021

Cells

View full text Add to dashboard Cite

Intergeneric crosses between Brassica species and Raphanus sativus have produced crops with prominent shoot and root systems of Brassica and R. sativus, respectively. It is necessary to discriminate donor genomes when studying cytogenetic stability in distant crosses to identify homologous chromosome pairing, and microsatellite repeats have been used to discriminate subgenomes in allopolyploids. To identify genome-specific microsatellites, we explored the microsatellite content in three Brassica species (B. rapa, AA, B. oleracea, CC, and B. nigra, BB) and R. sativus (RR) genomes, and validated their genome specificity by fluorescence in situ hybridization. We identified three microsatellites showing A, C, and B/R genome specificity. ACBR_msat14 and ACBR_msat20 were detected in the A and C chromosomes, respectively, and ACBR_msat01 was detected in B and R genomes. However, we did not find a microsatellite that discriminated the B and R genomes. The localization of ACBR_msat20 in the 45S rDNA array in ×Brassicoraphanus 977 corroborated the association of the 45S rDNA array with genome rearrangement. Along with the rDNA and telomeric repeat probes, these microsatellites enabled the easy identification of homologous chromosomes. These data demonstrate the utility of microsatellites as probes in identifying subgenomes within closely related Brassica and Raphanus species for the analysis of genetic stability of new synthetic polyploids of these genomes.

show abstract

“…The minimum interaction theory (MIT) (Imai et al ., 1988) has been used to explain karyotype evolution in Hymenoptera, which includes Apidae (Hoshiba and Imai, 1993; Pompolo and Campos, 1995; Rocha et al ., 2003; Godoy et al ., 2013; Santos et al ., 2018). This theory predicts that the chromosome number increased from a low‐numbered ancestral karyotype of 2 n ˂ 24 due to centric fission events and concomitant heterochromatin amplification, which stabilizes the new telomeres (Imai et al ., 1988).…”

Section: Introductionmentioning

confidence: 99%

Robertsonian rearrangements in Neotropical Meliponini karyotype evolution (Hymenoptera: Apidae: Meliponini)

Cunha

Soares

Clarindo

et al. 2021

Insect Molecular Biology

View full text Add to dashboard Cite

Genome changes, evidenced through karyotype or nuclear genome size data, can result in reproductive isolation, diversification and speciation. The aim of this study was to understand how changes in the karyotype such as chromosome number and nuclear genome size accompanied the evolution of neotropical stingless bees, and to discuss these data in a phylogenetic context focusing on the karyotype evolution of this clade. We sampled 38 species representing the three Neotropical Meliponini groups; 35 for karyotype analyses and 16 for 1C value measurement. The chromosome number varied from 2n = 16 to 2n = 34, with distinct karyotypic formulae and the presence of a few polymorphisms, such as B chromosomes in one species and arm size differences between homologous chromosomes in two species. The mean 1C value varied from 0.31 pg to 0.92 pg. We associated empirical data on chromosome number and mean 1C value to highlight the importance of Robertsonian fusion rearrangements, leading to a decrease in chromosome number during the Neotropical Meliponini evolution. These data also allowed us to infer the independent heterochromatin amplification in several genera. Although less frequent, Melipona species with 2n = 22 represent evidence of Robertsonian fissions. We also pointed out the importance of chromosomal rearrangements that did not alter chromosome number, such as inversions and heterochromatin amplification.

show abstract

Heterochromatin Distribution and Chromosomal Mapping of Microsatellite Repeats in the Genome ofFrieseomelittaStingless Bees (Hymenoptera: Apidae: Meliponini)

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 23 publications

References 25 publications

First Record of a B Chromosome in <b><i>Polybia fastidiosuscula</i></b> Saussure (Vespidae) and Investigation of Chromatin Composition Through Microsatellite Mapping

First Record of a B Chromosome in <b><i>Polybia fastidiosuscula</i></b> Saussure (Vespidae) and Investigation of Chromatin Composition Through Microsatellite Mapping

Subgenome Discrimination in Brassica and Raphanus Allopolyploids Using Microsatellites

Robertsonian rearrangements in Neotropical Meliponini karyotype evolution (Hymenoptera: Apidae: Meliponini)

Contact Info

Product

Resources

About