Heterochromatin of the scarab beetle, Bubas bison (Coleoptera: Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies

Colomba, Mariastella; Vitturi, R.; Libertini, Angelo; Gregorini, Armando; Zunino, Mario

doi:10.1016/j.micron.2005.06.004

Cited by 25 publications

(28 citation statements)

References 36 publications

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“…In the Scarabaeidae family and in Coleoptera as a whole, the small blocks of heterochromatin in the pericentromeres represent a conspicuous pattern that has been described in representative organisms from distinct and unrelated families (Moura et al, 2003;Bione et al, 2005a). On the other hand, some species had small additional blocks, as observed in D. geminatus, as was reported in Scarabaeidae (that is, Bubas bison) (Colomba et al, 2006) and Aphodius representative species (Wilson and Angus, 2004). Moreover, in species belonging to the subfamily Scarabaeinae, large heterochromatic blocks were observed, as described for Diabroctis mimas and Isocopris inhiata (Bione et al, 2005b).…”

Section: Discussionmentioning

confidence: 61%

“…The presence of only one nucleolar organizer region site detected by silver nitrate staining in D. geminatus does not correspond to the real genome organization of 45S rDNA clusters (2 clusters of 18S rDNA detected by FISH). The presence of more than one 45S rDNA site was observed in other Scarabaeinae species, such as Bubas bison (Colomba et al, 2006) and D. mimas (Bione et al, 2005b), and in unrelated groups of Coleoptera, such as Cicindelidae and Scarabaeidae as a whole, indicating more than one dispersion event of rDNA sequences.…”

Section: Mapping Of Repetitive Dnas In D Geminatus Dc Cabral-de-mellmentioning

confidence: 94%

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Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

2009

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Chromosomal banding techniques and repetitive DNA mapping are useful tools in comparative analysis and in the elucidation of genome organization of several groups of eukaryotes. In this study, we contributed to the knowledge of Coleoptera genomes by reporting the chromosomal organization of repetitive DNA sequences, as well as the presence and characteristics of a B chromosome in two natural populations of Dichotomius geminatus (Coleoptera; Scarabaeidae) using classical, chromosomal banding and molecular cytogenetic techniques. As in other coleopteran species, the heterochromatin was mainly concentrated in pericentromeric regions and the B chromosome was composed almost entirely of heterochromatin. Physical mapping using double fluorescent in situ hybridization was performed for the first time in Coleoptera; using DNA probes for 5S and 18S ribosomal RNA (rRNA) and histone H3 genes, we showed that ribosomal 18S rDNAs are located in chromosomes 3 and 4, whereas 5S rRNA and histone H3 genes are colocalized in chromosomal pair 2 and show an apparently interspersed organization. Moreover, these genes are not present in the B chromosome, suggesting that the B chromosome did not originate from chromosomal pairs 2, 3 or 4. On the other hand, mapping of the C 0 t-1 DNA fraction showed that the B chromosome is enriched in repetitive DNA elements, also present in the standard complement, indicating an intraspecific origin of this element in D. geminatus. These results will contribute to our understanding of genome organization and evolution of repetitive elements in Coleoptera and other insects regarding both A and B chromosomes.

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Section: Discussionmentioning

confidence: 61%

Section: Mapping Of Repetitive Dnas In D Geminatus Dc Cabral-de-mellmentioning

confidence: 94%

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

2009

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“…The C-banding pattern observed in E. caribaeus is quite common among Scarabaeidae representatives and also to Coleoptera as a whole (Moura et al 2003;Colomba et al 2004Colomba et al , 2006Rozek et al 2004;Wilson and Angus 2004, 2005Bione et al 2005b;Schneider et al 2006). Meanwhile, particular cases of CH distribution have been reported in some Scarabaeidae; Cetonia aurata and Bubas bison have CH blocks located in the terminal region of eight autosomal bivalents, in addition to the pericentromeric blocks (Colomba et al 1996(Colomba et al , 2006Dutrillaux et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, particular cases of CH distribution have been reported in some Scarabaeidae; Cetonia aurata and Bubas bison have CH blocks located in the terminal region of eight autosomal bivalents, in addition to the pericentromeric blocks (Colomba et al 1996(Colomba et al , 2006Dutrillaux et al 2006). In some species of Scarabaeidae, such as Diabroctis mimas and Isocopris inhiata, the occurrence of diphasic chromosomes that present one heterochromatic and another euchromatic arm has been described (Bione et al 2005a).…”

Section: Discussionmentioning

confidence: 99%

“…The constitutive heterochromatin (CH) in this family is predominantly located in the pericentric region of the chromosomes and this genomic component shows wide heterogeneity regarding AT-richness and GC-richness. Moreover, species with telomeric, interstitial CH and diphasic chromosomes have been described in this family (Colomba et al 1996(Colomba et al , 2000(Colomba et al , 2006Moura et al 2003;Bione et al 2005a;Angus et al 2007;Macaisne et al 2006). The nucleolar organizer regions (NORs) are predominantly located in a single autosomal pair or in the X chromosome.…”

Section: Introductionmentioning

confidence: 98%

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Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion

Arcanjo

Cabral-de-Mello

Silva

et al. 2009

J Genet

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Mitotic and meiotic chromosomes of several populations of Eurysternus caribaeus (Coleoptera: Scarabaeidae) were analysed through conventional staining, C-banding, base-specific fluorochromes, silver nitrate staining and fluorescent in situ hybridization (FISH). All specimens showed 2n = 8 in their karyotypes, with a neo-XY sex system (Y is a submetacentric and X a metacentric) and three pairs of submetacentric autosomes. The analysis of constitutive heterochromatin (CH) revealed small blocks located in the centromeric region of all chromosomes which do not present positive staining under the fluorochromes CMA 3 and DAPI. Silver nitrate staining revealed that the nucleolar organizer region (NORs) is associated with the sex chromosomes. The FISH technique revealed that rDNA sites in the X and Y are different in size. Data from different populations indicate that the diploid number reduction (2n = 8) observed in E. caribaeus is established and presumably has preceded the dispersion of this species. Moreover, this reduction occasioned the translocation of rDNA sites to the sex chromosomes, X and Y, an uncommon pattern in Scarabaeidae that was observed for the first time by the FISH in this work.

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Chromosomal study of a lamprey (Lampetra zanandreai Vladykov, 1955) (Petromyzonida: Petromyzontiformes): conventional and FISH analysis

et al. 2011

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Karyotype and other chromosomal characteristics in the Adriatic brook lamprey Lampetra zanandreai, representative of one of the most ancestral group of vertebrates, were examined using conventional (Ag-staining, C-banding as well as CMA(3) and DAPI fluorescence) and molecular (FISH with 18/28S rDNA and EcoRI satDNA as probes) protocols with metaphase chromosomes derived from whole blood cultures. The chromosome complement had a modal diploid chromosome number of 2n = 164, as in other petromyzontid lamprey species. Ag-staining and CMA(3) fluorescence, as well as FISH with 18/28S rDNA probes, detected nucleolar organizer regions (NORs) close to the centromeres of the biarmed chromosomes of pairs 1 and 2, the largest chromosome pairs of the complement. In addition to NORs, CMA(3) fluorescence revealed positive signals in approximately 40 other chromosomes. DAPI stained mostly centromeric regions of many chromosomes as well as conspicuously massive blocks overlapping NOR sites. C-banding evidenced a large amount of constitutive heterochromatin in somatic chromosomes, with approximately 40 C-positive acrocentric elements completely heterochromatic, corresponding with the 40 CMA(3)+ chromosomes and positive heterochromatic blocks in pericentromeric regions of chromosome pairs 1 and 2. Polymerase chain reaction (PCR)-based cloning of satellite DNA with primers derived from Petromyzon marinus centromeric sequences was successful for L. zanandreai genomic DNA. The sequence was AT-rich (59%) and characterized by short consensus motifs similar to other centromeric satellite motifs. FISH using satDNA clones as a probe produced a fluorescent signal on a single pair of small chromosomes. This sequence was PCR-amplified also in L. planeri and P. marinus genomic DNA, and the evolution of this repetitive element in the above species was analysed.

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Heterochromatin of the scarab beetle, Bubas bison (Coleoptera: Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA copies

Cited by 25 publications

References 36 publications

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

Cytogenetic characterization of Eurysternus caribaeus (Coleoptera: Scarabaeidae): evidence of sex-autosome fusion and diploid number reduction prior to species dispersion

Chromosomal study of a lamprey (Lampetra zanandreai Vladykov, 1955) (Petromyzonida: Petromyzontiformes): conventional and FISH analysis

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