Heterochromatin Positioning and Nuclear Architecture

Complex sequence organization of heterochromatin in the holocentric plant Cuscuta europaea elucidated by the computational analysis of nanopore reads

“…Heterochromatin is a tightly packed, fundamental form of chromatin organization in eukaryotic nuclei exhibiting a unique combination of post-translational histone modifications [1] , [49] . In higher plants, cytologically defined constitutive heterochromatin is mostly associated with large tracks of highly repetitive satellite DNA (satDNA) and forms densely stained bands on mitotic chromosomes or chromocenters in interphase nuclei [13] .…”

“…In higher plants, cytologically defined constitutive heterochromatin is mostly associated with large tracks of highly repetitive satellite DNA (satDNA) and forms densely stained bands on mitotic chromosomes or chromocenters in interphase nuclei [13] . In plants with monocentric chromosomes and small genomes, this heterochromatin is usually confined to centromeric and pericentric regions [49] . In species with larger genomes, however, it can be found in additional subtelomeric and interstitial chromosomal loci [12] , whereas plants with holocentric chromosomes usually lack distinguishable heterochromatic bands [19] .…”

“…In species with larger genomes, however, it can be found in additional subtelomeric and interstitial chromosomal loci [12] , whereas plants with holocentric chromosomes usually lack distinguishable heterochromatic bands [19] . Heterochromatin is supposed to play an important role in chromosome segregation, gene regulation and the maintenance of genome stability [49] , yet the processes shaping its distribution throughout the genome, and the role of underlying repetitive sequences, remain poorly understood [13] . This is in part due to our limited knowledge of the long-range sequence arrangement of repeat-rich heterochromatic regions which are in principle difficult to assemble [38] .…”

Response of isolated buccal epithelium cells on the combined action of anti-tumor antibiotic doxorubicin and magnetic field

“…Усього оброблено по 300 клітин у будь-якому варіанті експерименту (три повторності по 100 клітин). Як відомо, формування гранул гетерохроматину (а саме факультативного гетерохроматину) є загальною реакцією клітини на стрес, фізіологічні чи екологічні чинники [15,20].…”