Heterocyclic Alkaloids as Antimicrobial Agents of Aspergillus fumigatus D Endophytic on Edgeworthia chrysantha

Zhang, Huawei; Ruan, Chuanfen; X, Bai; Chen, Jianwei; Wang, Hong

doi:10.1007/s10600-018-2365-4

Cited by 17 publications

(12 citation statements)

References 13 publications

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“…Chemical investigation of one symbiotic stain Aspergillus sp. D from Edgeworthia chrysantha led to isolation of five known heterocyclic alkaloids from normal Czapek medium, while a new meroterpenoid ( 108 ) and four known analogs were obtained from Czapek medium with 3% salty (Zhang et al, 2018a,b). One mangrove-derived endophyte Wallemia sebi PXP-89 cultivated in 10% NaCl broth produced a new cyclopentanol pyridine alkaloid ( 109 ), which was not detected in normal medium (Peng et al, 2011).…”

Section: Variation Of Mediummentioning

confidence: 99%

Exploring Structural Diversity of Microbe Secondary Metabolites Using OSMAC Strategy: A Literature Review

et al. 2019

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Microbial secondary metabolites (MSMs) have played and continue to play a highly significant role in the drug discovery and development process. Genetically, MSM chemical structures are biologically synthesized by microbial gene clusters. Recently, however, the speed of new bioactive MSM discovery has been slowing down due to consistent employment of conventional cultivation and isolation procedure. In order to alleviate this challenge, a number of new approaches have been developed. The strategy of one strain many compounds (OSMAC) has been shown as a simple and powerful tool that can activate many silent biogenetic gene clusters in microorganisms to make more natural products. This review highlights important and successful examples using OSMAC approaches, which covers changing medium composition and cultivation status, co-cultivation with other strain(s), adding enzyme inhibitor(s) and MSM biosynthetic precursor(s). Available evidences had shown that variation of cultivation condition is the most effective way to produce more MSMs and facilitate the discovery of new therapeutic agents.

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Section: Variation Of Mediummentioning

confidence: 99%

Exploring Structural Diversity of Microbe Secondary Metabolites Using OSMAC Strategy: A Literature Review

et al. 2019

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“…It was identified as A. fumigatus using its morphological characteristics and analysis of 18S rDNA gene sequence (GenBank accession No. KR019681) as described before [6]. Previous chemical investigation indicates that strain D could grow well in salted Czapek medium with 3% or 10% NaCl, and produce various secondary metabolites, which had been reported [7,8].…”

Section: Strain Medium and Cultural Conditionsmentioning

confidence: 96%

“…KR019681) [5]. Bioassay-guided fractionation of the ethyl acetate extract of strain D, cultured in salted and unsalted Czapek media, previously led to the isolation of seven antimicrobial agents, including bisdethiobis (methylthio) gliotoxin, gliotoxin, pseurotin A, and spirotryprostatins A and G, ACTG-toxin F, and 8-chloro-3,6a,7,9,10-pentahydroxy-9,8,7,6a-tetrahydroperylen-4(6aH)-one [6][7][8]. In our continuous search for more bioactive SMs from strain D using the "one strain many compounds" (OSMAC) strategy [9], chemical investigation was further carried out in this work.…”

Section: Introductionmentioning

confidence: 99%

“…Rubrofusarin B (1): Yellowish amorphous powder; C 16 H 14 O 5 ; 1 H NMR (600 MHz, CDCl 3 ): 2.38 (3H, s), 3.94 (3H, s), 4.02 (3H, s), 6.01 (1H, s), 6.41 (1H, d, J = 2.35),6.60 (1H, d, J = 2.35),6.98 (1H, s), 14.98 (1H, s, 5-OH) 13. C NMR (600 MHz, CDCl 3 ): 23.81, 64.38, 66.30, 97.37, 97.93, 101.15, 104.50, 107.45, 141.40, 149.18, 160.76, 161.61, 162.76, 167.80, 183.90.…”

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Aromatic Polyketides from a Symbiotic Strain Aspergillus fumigatus D and Characterization of Their Biosynthetic Gene D8.t287

Hua

Pan

et al. 2020

Marine Drugs

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The chemical investigation of one symbiotic strain, Aspergillus fumigatus D, from the coastal plant Edgeworthia chrysantha Lindl led to the isolation of eight compounds (1–8), which were respectively identified as rubrofusarin B (1), alternariol 9-O-methyl ether (2), fonsecinone D (3), asperpyrone A (4), asperpyrone D (5), fonsecinone B (6), fonsecinone A (7), and aurasperone A (8) by a combination of spectroscopic methods (1D NMR and ESI-MS) as well as by comparison with the literature data. An antimicrobial assay showed that these aromatic polyketides exhibited no remarkable inhibitory effect on Escherichia coli, Staphyloccocus aureus and Candida albicans. The genomic feature of strain D was analyzed, as well as its biosynthetic gene clusters, using antibiotics and Secondary Metabolite Analysis Shell 5.1.2 (antiSMASH). Plausible biosynthetic pathways for dimeric naphtho-γ-pyrones 3–8 were first proposed in this work. A non-reducing polyketide synthase (PKS) gene D8.t287 responsible for the biosynthesis of these aromatic polyketides 1–8 was identified and characterized by target gene knockout experiment and UPLC-MS analysis.

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“…The endophytic Aspergillus fumigatus extract exhibited a broad spectrum of antimicrobial activity against human pathogenic microbes 50 . Similarly, the antimicrobial evaluation of our endophyte isolate F. oxysporum showed significant antibacterial activity at p<0.05.…”

Section: Cultivation Of Fusarium Oxysporum and Antimicrobial Screeninmentioning

confidence: 99%

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Heterocyclic Alkaloids as Antimicrobial Agents of Aspergillus fumigatus D Endophytic on Edgeworthia chrysantha

Cited by 17 publications

References 13 publications

Exploring Structural Diversity of Microbe Secondary Metabolites Using OSMAC Strategy: A Literature Review

Exploring Structural Diversity of Microbe Secondary Metabolites Using OSMAC Strategy: A Literature Review

Aromatic Polyketides from a Symbiotic Strain Aspergillus fumigatus D and Characterization of Their Biosynthetic Gene D8.t287

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