Heterodimeric Interactions between Chicken Ovalbumin Upstream Promoter-Transcription Factor Family Members ARP1 and Ear2

Avram, Dorina; Ishmael, Jane E.; Nevrivy, Daniel J.; Peterson, Valerie J.; Lee, Suk-Hyung; Dowell, Paul; Leid, Mark

doi:10.1074/jbc.274.20.14331

Cited by 34 publications

(31 citation statements)

References 49 publications

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“…The mechanism(s) underlying transcriptional repression mediated by CTIP1 in mammalian cells is unknown but may involve interaction with and disruption of the function of the general transcription machinery (48,49), interaction with chromatin components, or remodeling factors resulting in stabilization of inactive chromatin structure (50), titration of transcriptional coactivators such as p300 and CBP (46), interaction with general corepressor proteins such as Groucho (51) Both CTIP1 and CTIP2, like the COUP-TF proteins, are highly expressed in the central nervous system and in the developing embryo (3,19,21,32), suggesting that CTIPs may mediate, at least in part, the activity of these orphan receptors during mammalian neurogenesis and organogenesis in tissues such as heart and liver. However, it is possible that CTIP proteins may also function independently of COUP-TF family members.…”

Section: Discussionmentioning

confidence: 99%

“…HEK293 cells (ATCC CRL 1573) were cultured and transfected as described previously (21). Culture of CATH.a cells (33) and preparation of mRNA from these cells have been described previously (34).…”

Section: Cell Culturementioning

confidence: 99%

“…Here we show that CTIP1, a member of a novel family of C 2 H 2 zinc finger proteins that was isolated as an ARP1 interaction partner, harbors autonomous transcriptional repression domains and potentiates ARP1-mediated transcriptional repression independently of trichostatin A-sensitive histone deacetylation. Both CTIP1 and the related CTIP2 are highly expressed in the brain, a tissue also known to express COUP-TF family members abundantly (3,21,32), suggesting that this novel family of C 2 H 2 zinc finger proteins may play a role in COUP-TF signaling.…”

mentioning

confidence: 99%

“…However, Ear2 heterodimerizes with both COUP-TFI and ARP1 in solution and on various, directly repeated DNA response elements (21), suggesting that Ear2 may be implicated in both COUP-TFI and ARP1 signaling pathways.…”

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confidence: 99%

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Isolation of a Novel Family of C2H2 Zinc Finger Proteins Implicated in Transcriptional Repression Mediated by Chicken Ovalbumin Upstream Promoter Transcription Factor (COUP-TF) Orphan Nuclear Receptors

Avram¹,

Fields²,

Top³

et al. 2000

Journal of Biological Chemistry

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Two novel and related C 2 H 2 zinc finger proteins that are highly expressed in the brain, CTIP1 and CTIP2 (COUP TF-interacting proteins 1 and 2, respectively), were isolated and shown to interact with all members of the chicken ovalbumin upstream promoter transcription factor (COUP-TF) subfamily of orphan nuclear receptors. The interaction of CTIP1 with ARP1 was studied in detail, and CTIP1 was found to harbor two independent ARP1 interaction domains, ID1 and ID2, whereas the putative AF-2 of ARP1 was required for interaction with CTIP1. CTIP1, which exhibited a punctate staining pattern within the nucleus of transfected cells, recruited cotransfected ARP1 to these foci and potentiated ARP1-mediated transcriptional repression of a reporter construct. However, transcriptional repression mediated by ARP1 acting through CTIP1 did not appear to involve recruitment of a trichostatin A-sensitive histone deacetylase(s) to the template, suggesting that this repression pathway may be distinct from that utilized by several other nuclear receptors.COUP-TFI, 1 ARP1/COUP-TFII, and Ear2/COUP-TFIII have been grouped in the same subfamily of orphan nuclear receptors based on sequence similarity (1-3), evolutionary analysis (4), and a common capacity to repress ligand-dependent transcriptional activation of target genes mediated by other nuclear receptors, such as retinoic acid (5-10), thyroid hormone (8), estrogen (11)(12)(13)(14), and vitamin D 3 (9) receptors as well as peroxisome proliferator-activated receptor α (PPARα;Ref. 15).COUP-TFs play important roles in pattern formation in the developing nervous systems of Xenopus (16) and Drosophila (17). Deletion of the COUP-TFI gene in the mouse results in * This work was supported in part by American Heart Association Grant 9640219N; NIEHS, National Institutes of Health, Grants ES00210 and ES00040; the Oregon State University College of Pharmacy; and the Laboratory of Molecular Pharmacology. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.© 2000 by The American Society for Biochemistry and Molecular Biology, Inc. ∥ An established investigator of the American Heart Association. To whom correspondence and reprint requests should be addressed: MATERIALS AND METHODS Yeast Two-hybrid Screening and cDNA CloningYeast two-hybrid screening was conducted as described previously (21) using the hinge region and putative ligand binding domain of ARP1 (amino acids 144-414) as a bait. Fragments corresponding to CTIP1 and CTIP2 (see Fig. 1A) were used to screen mouse cDNA libraries obtained from CLON-TECH and from Dr. René Hen (Columbia University), yielding several overlapping clones. These overlapping clones were then used to prepare a full-length CTIP1 construct that was inserted into the eukaryotic expression vector, pCDNA3+ (Invitrogen). Yeast β-Galactosidase Assays and GST Pull-down ExperimentsProtein-p...

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Section: Discussionmentioning

confidence: 99%

Section: Cell Culturementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation of a Novel Family of C2H2 Zinc Finger Proteins Implicated in Transcriptional Repression Mediated by Chicken Ovalbumin Upstream Promoter Transcription Factor (COUP-TF) Orphan Nuclear Receptors

Avram¹,

Fields²,

Top³

et al. 2000

Journal of Biological Chemistry

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187

205

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“…Ear2 can homo-or hetero-dimerize with COUP-TFI and COUP-TFII (Avram et al, 1999) and bind to enhancers in the sequence of various genes. Ear2À/À mice lack the major part of the locus coeruleus, which provides the majority of noradrenergic transmission in mammals (Warnecke et al, 2005) and contacts a great number of brain structures, particularly in the forebrain.…”

Section: Noradrenergic Projections As Possible Routes From or To The Fecmentioning

confidence: 99%

“Feeding time” for the brain: A matter of clocks

Feillet

Albrecht

Challet

2006

Journal of Physiology-Paris

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Circadian clocks are autonomous time-keeping mechanisms that allow living organisms to predict and adapt to environmental rhythms of light, temperature and food availability. At the molecular level, circadian clocks use clock and clock-controlled genes to generate rhythmicity and distribute temporal signals. In mammals, synchronization of the master circadian clock located in the suprachiasmatic nuclei of the hypothalamus is accomplished mainly by light stimuli. Meal time, that can be experimentally modulated by temporal restricted feeding, is a potent synchronizer for peripheral oscillators with no clear synchronizing influence on the suprachiasmatic clock. Furthermore, food-restricted animals are able to predict meal time, as revealed by anticipatory bouts of locomotor activity, body temperature and plasma corticosterone. These food anticipatory rhythms have long been thought to be under the control of a food-entrainable clock (FEC). Analysis of clock mutant mice has highlighted the relevance of some, but not all of the clock genes for foodentrainable clockwork. Mutations of Clock or Per1 do not impair expression of food anticipatory components, suggesting that these clock genes are not essential for food-entrainable oscillations. By contrast, mice mutant for Npas2 or deficient for Cry1 and Cry2 show more or less altered responses to restricted feeding conditions. Moreover, a lack of food anticipation is specifically associated with a mutation of Per2, demonstrating the critical involvement of this gene in the anticipation of meal time. The actual location of the FEC is not yet clearly defined. Nevertheless, current knowledge of the putative brain regions involved in food-entrainable oscillations is discussed. We also describe several neurochemical pathways, including orexinergic and noradrenergic, likely to participate in conveying inputs to and outputs from the FEC to control anticipatory processes.

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Hepatic Nuclear Receptors

Soccio

2020

The Liver

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Heterodimeric Interactions between Chicken Ovalbumin Upstream Promoter-Transcription Factor Family Members ARP1 and Ear2

Cited by 34 publications

References 49 publications

Isolation of a Novel Family of C2H2 Zinc Finger Proteins Implicated in Transcriptional Repression Mediated by Chicken Ovalbumin Upstream Promoter Transcription Factor (COUP-TF) Orphan Nuclear Receptors

Isolation of a Novel Family of C2H2 Zinc Finger Proteins Implicated in Transcriptional Repression Mediated by Chicken Ovalbumin Upstream Promoter Transcription Factor (COUP-TF) Orphan Nuclear Receptors

“Feeding time” for the brain: A matter of clocks

Hepatic Nuclear Receptors

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