2020
DOI: 10.1186/s13068-020-01693-0
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Heteroexpression of Osa-miR319b improved switchgrass biomass yield and feedstock quality by repression of PvPCF5

Abstract: Background: Switchgrass (Panicum virgatum L.), a C 4 perennial grass, has been recognized as one of the most potentially important lignocellulose biofuel crops. MicroRNA319 (miR319) plays a key role in plant development, abiotic resistance, and cell wall biosynthesis by repressing expression of its target TCP genes. We hypothesized miR319-TCP pathway could play important roles in switchgrass feedstock characteristics for biofuel production, and produced switchgrass transgenic plants overexpressing miR319 (by e… Show more

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Cited by 14 publications
(19 citation statements)
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“…It should be noted that the expression of miR396 was not tightly associated with how much its targeted PvGRFs were repressed in the tissues analysed, further suggesting a possible complicated relationship between miR396 and PvGRFs in switchgrass. Similarly, a loose negative effect of miR319 on the expression of its targeted genes in different tissues has previously been reported in switchgrass and rice (Liu et al, 2020;Yang et al, 2013).…”
Section: Discussionsupporting
confidence: 70%
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“…It should be noted that the expression of miR396 was not tightly associated with how much its targeted PvGRFs were repressed in the tissues analysed, further suggesting a possible complicated relationship between miR396 and PvGRFs in switchgrass. Similarly, a loose negative effect of miR319 on the expression of its targeted genes in different tissues has previously been reported in switchgrass and rice (Liu et al, 2020;Yang et al, 2013).…”
Section: Discussionsupporting
confidence: 70%
“…The individual rPvGRFs were subcloned into pZh01, respectively. To repress the function of PvGRFs, the 3’ ends of PvGRFs were linked to a sequence encoding the SRDX domain and cloned into pZh01 (Liu et al, 2020 ; Wu et al, 2016 ). All of these genes were under the control of the CaMV 35S promoter.…”
Section: Methodsmentioning
confidence: 99%
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“…miR319 has been demonstrated to promote cell proliferation during leaf and petal development ( Nag et al, 2009 ; Liu et al, 2020 ); however, the effect of miR319 on stem elongation varies with plant species. Overexpression of sha-miR319d in tomato reduced plant height ( Shi et al, 2019 ), whereas overexpression of osa-miR319b in switchgrass promoted stem elongation and increased plant height ( Liu et al, 2020 ). Knockdown of miR319 using artificial miRNA target mimics (MIM) completely suppressed stem elongation in Arabidopsis ( Todesco et al, 2010 ).…”
Section: Discussionmentioning
confidence: 99%