Heterogeneities in Ventricular Conduction Following Treatment with Heptanol: A Multi-Electrode Array Study in Langendorff-Perfused Mouse Hearts

Dong, Xiuming; Tse, Gary; Hao, Guoliang; Du, Yumin

doi:10.3390/life12070996

Cited by 5 publications

(4 citation statements)

References 54 publications

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“…Furthermore, the conduction inhomogeneity index can be employed to differentiate between both types of abnormalities. 34 The findings of this study show that the absolute inhomogeneity and conduction inhomogeneity index change synchronously, demonstrating that the change in inhomogeneity after hypothermic global ischemia-reperfusion is primary. A study has observed that the rat hearts resumed sinus rhythm and conduction within 10 to 15 minutes after 60 minutes of arrests at room temperature.…”

Section: Discussionsupporting

confidence: 52%

Sevoflurane Improves Ventricular Conduction by Exosomes Derived from Rat Cardiac Fibroblasts After Hypothermic Global Ischemia-Reperfusion Injury

Ma¹,

Cao²,

Gao³

et al. 2023

DDDT

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This study investigated the effect of exosomes derived from sevoflurane-treated cardiac fibroblasts (Sev-CFs-Exo) on reperfusion arrhythmias (RA), ventricular conduction, and myocardial ischemia-reperfusion injury (MIRI). Methods: Primary cardiac fibroblasts (CFs) were isolated from the hearts of neonatal rats and identified by morphology and immunofluorescence. Exosomes were isolated from CFs at passages 2-3 after they had been treated with 2.5% sevoflurane for an hour and cultivated for 24-48 hours. The control group was CFs that did not receive any treatment. The hypothermic global ischemia-reperfusion injury model was established using the Langendorff perfusion technique following injection with exosomes through the caudal vein. Multi-electrode array (MEA) mapping was used to investigate the changes in RA and ventricular conduction in isolated hearts. Western blots and immunofluorescence were used to examine the relative expression and location of connexin 43 (Cx43). In addition, the MIRI was evaluated with triphenyl tetrazolium chloride and Hematoxylin-Eosin staining. Results: The primary CFs had a variety of morphologies, no spontaneous pulsation, and were vimentin-positive, which confirmed their successful isolation. Sev-CFs-Exo increased the heart rate (HR) at reperfusion for 15 minutes (T 2 ) and 30 minutes (T 3 ) and lowered the score and duration of RA and the time for restoration of heartbeat in reperfusion. Meanwhile, Sev-CFs-Exo increased conduction velocity (CV), decreased absolute inhomogeneity (P 5-95 ) and inhomogeneity index (P 5-95 /P 50 ) at T 2 and T 3 , as well as promoted the recovery of HR, CV, P 5-95 and P 5-95 /P 50 after hypothermic global ischemia-reperfusion injury. Furthermore, Sev-CFs-Exo raised expression and reduced lateralization of Cx43, and improved myocardial infarct sizes and cellular necrosis. However, while cardiac fibroblast-derived exosomes (CFs-Exo) showed similar cardioprotective effects, the outcomes were not as significant. Conclusion: Sevoflurane reduces the risk of RA and improves ventricular conduction and MIRI by CFs-Exo, and this may be driven by the expression and location of Cx43.

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Section: Discussionsupporting

confidence: 52%

Sevoflurane Improves Ventricular Conduction by Exosomes Derived from Rat Cardiac Fibroblasts After Hypothermic Global Ischemia-Reperfusion Injury

Ma¹,

Cao²,

Gao³

et al. 2023

DDDT

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“…Using mice anesthetized with 1.5% tribromoethanol (0.02 mL/g; WXBD3759V, Sigma, St. Louis, Missouri, USA), and supported by a tracheal intubation ventilator, mouse chests were surgically opened with full exposure to the heart. Following this, the pericardium was removed, epicardial conduction velocity (CV), absolute inhomogeneity and inhomogeneity index was recorded and analyzed using the Electrical Mapping System (EMS64-USB-1003, MappingLab, Oxford, UK) and EMapScope 4.0 (MappingLab, Oxford, UK), as detailed previously [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Sacubitril/Valsartan Ameliorates Crizotinib-Induced Cardiotoxicity in Mice

Cheng¹,

Duan²,

Tse³

et al. 2023

Rev. Cardiovasc. Med.

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Background: Lung cancer is one of the major cause of death globally. Crizotinib is a first-line drug used in treating non-small-cell lung cancer (NSCLC). However, the pathophysiological mechanisms underlying its cardiotoxicity are unknown. This study investigated the mechanisms of crizotinib-induced cardiotoxicity and explored whether this toxicity can be prevented by the angiotensin receptor/neprilysin inhibitor sacubitril/valsartan. Methods: Male C57BL/6 mice were randomly divided into three groups: control, crizotinib (40 mg for four weeks), and crizotinib + sacubitril/valsartan (40 mg /60 mg for four weeks). Expression of genes in myocardial tissue were detected by transcriptomic sequencing, with verification of the differentially expressed genes (DEGs) using Real time-polymerase chain reaction (RT-PCR). Blood pressure (BP) and cardiac function of animals were measured using non-invasive monitoring and echocardiography approaches. Ventricular refractory period (RP), as well as the induction rate and score of ventricular arrhythmias (VAs) were detected by in vivo electrophysiology. Epicardial conductance was measured by mapping. Expression of Myh7 in myocardium was detected by western blot and RT-PCR. Results: DEGs detected using transcriptomic sequencing included 10 up-regulated and 20 down-regulated genes. The first 5 DEGs identified were Myh7 , Ngp , Lcn2 , Ciart and Ptgds . Kyoto Encyclopedia of Genes and Genomes (KEGG) result indicated that Myh7 is involved in myocarditis, cardiomyopathy, and cardiac muscle contraction. Crizotinib treatment increased blood pressure, prolonged QTc interval, shortened ventricular RP, increased the incidence and score of right VAs, and increased Myh7 expression. Most of these responses were limited by sacubitril/valsartan. Conclusions: Crizotinib induced a range of cardiotoxic side effects in a mouse model and increased Myh7 expression represents a biomarker for this response. These cardiovascular toxic responses can be largely prevented by sacubitril/valsartan.

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“…In addition to all these indicators, the most widely employed measurement of heterogeneity is the Spatial Inhomogeneity index, described by Lammers in 1990 [21] . This metric, based on conduction delays across the tissue, has been applied to high-density multielectrode recordings to evaluate arrhythmogenicity [22] , [23] , [24] .…”

Section: Introductionmentioning

confidence: 99%

Vector Field Heterogeneity for the Assessment of Locally Disorganised Cardiac Electrical Propagation Wavefronts From High-Density Multielectrodes

Pancorbo,

Ruipérez-Campillo,

Tormos

et al. 2024

IEEE Open J. Eng. Med. Biol.

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High-density multielectrode catheters are becoming increasingly popular in cardiac electrophysiology for advanced characterisation of the cardiac tissue, due to their potential to identify impaired sites. These are often characterised by abnormal electrical conduction, which may cause locally disorganised propagation wavefronts. To quantify it, a novel heterogeneity parameter based on vector field analysis is proposed, utilising finite differences to measure direction changes between adjacent cliques. The proposed Vector Field Heterogeneity metric has been evaluated on a set of simulations with controlled levels of organisation in vector maps, and a variety of grid sizes. Furthermore, it has been tested on animal experimental models of isolated Langendorff-perfused rabbit hearts. The proposed parameter exhibited superior capturing ability of heterogeneous propagation wavefronts compared to the classical Spatial Inhomogeneity Index, and simulations proved that the metric effectively captures gradual increments in disorganisation in propagation patterns. Notably, it yielded robust and consistent outcomes for \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} $\mathbf {4\times 4}$\end{document} grid sizes, underscoring its suitability for the latest generation of orientation-independent cardiac catheters.

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Heterogeneities in Ventricular Conduction Following Treatment with Heptanol: A Multi-Electrode Array Study in Langendorff-Perfused Mouse Hearts

Cited by 5 publications

References 54 publications

Sevoflurane Improves Ventricular Conduction by Exosomes Derived from Rat Cardiac Fibroblasts After Hypothermic Global Ischemia-Reperfusion Injury

Sevoflurane Improves Ventricular Conduction by Exosomes Derived from Rat Cardiac Fibroblasts After Hypothermic Global Ischemia-Reperfusion Injury

Sacubitril/Valsartan Ameliorates Crizotinib-Induced Cardiotoxicity in Mice

Vector Field Heterogeneity for the Assessment of Locally Disorganised Cardiac Electrical Propagation Wavefronts From High-Density Multielectrodes

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