Heterogeneity among Homologs of Cutinase-Like Protein Cut5 in Mycobacteria

Verma, Deepshikha; Das, Lahari; Gambhir, Vandana; Dikshit, Kanak L.; Varshney, Grish C.

doi:10.1371/journal.pone.0133186

Cited by 6 publications

(1 citation statement)

References 44 publications

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“…A family of enzymes Cytochrome P450 is important in the metabolism of steroids, fat‐soluble vitamins, carcinogens, pesticides, and many other types of chemicals, and responsible for around three‐quarters of all drug metabolism reactions 48 . Cutinases is thought to be involved in various metabolic pathways and physiological functions in mycobacteria 49 . Here, Cutinase1 (CUT1) showed direct or indirect connections to seven cytochrome P450 enzymes.…”

Section: Resultsmentioning

confidence: 99%

Characterization of O‐mannosylated proteins profiling in bacillus Calmette–Guérin via gel‐based and gel‐free approaches

et al. 2021

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Posttranslational modifications (PTMs) could influence many aspects of protein behavior and function in organisms. Protein glycosylation is one of the major PTMs observed in bacteria, which is crucial for functional regulations of many prokaryotic and eukaryotic organisms. Mycobacterium bovis bacillus Calmette–Guérin (BCG) vaccine has been recognized as an indispensable tool in the global fight against tuberculosis (TB) worldwide over several decades. Nevertheless, analysis of glycoprotein profiles of BCG has not been clearly investigated. In this study, we performed O‐mannosylated protein analysis in BCG bacteria using gel‐based and gel‐free approaches. In total, 1,670 hexosylated peptides derived from 754 mannosylated proteins were identified. Furthermore, 20 novel protein products supported by 78 unique peptides not annotated in the BCG database were detected. Additionally, the translational start sites of 384 proteins were confirmed, and 78 proteins were validated through the extension of translational start sites based on N‐terminus‐derived peptides. The bioinformatic analysis of the O‐mannosylated proteins was performed and the expression profiles of four randomly selected proteins were validated through Western blotting. A number of proteins involved in metabolic pathways, including the tricarboxylic acid cycle, glycolysis, oxidative phosphorylation, and two‐component system, are discussed. Taken together, these results offer the first O‐mannosylated protein analysis of a member of mycobacteria reported to date by using complementary gel‐based and gel‐free approaches. Some of the proteins identified in this study have important roles involved in metabolic pathways, which could provide insight into the immune molecular mechanisms of this recognized vaccine strain.

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Section: Resultsmentioning

confidence: 99%

Characterization of O‐mannosylated proteins profiling in bacillus Calmette–Guérin via gel‐based and gel‐free approaches

et al. 2021

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Roles of Triolein and Lipolytic Protein in the Pathogenesis and Survival of Mycobacterium tuberculosis: a Novel Therapeutic Approach

Monu

Meena

2015

Appl Biochem Biotechnol

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Discovery of novel secreted enzymes and proteins in Mycobacterium tuberculosis (M. tuberculosis) are imperative to understanding the pathogenic system for pathogenesis requires attention. Till date, the groups of these secreted enzymes are not meaningfully characterized in terms of M. tuberculosis. In this way, cutinase, a small lipolytic protein, exists in both bacteria and fungi as well which have a potential catalytic activity. During our search, we have found a few genes of M. tuberculosis demonstrating a same significant lipase action as fungi Fusarium solani cutinase contain. Genome sequencing of M. tuberculosis uncover a lot of proteins, wherein (Rv1758, Rv1984c, Rv2301, Rv3451, Rv3452, Rv3724A, Rv3724B, and Rv3802c) genes have been noted which are exhibiting a cutinase-like activity and closely homologous to that of F. solani cutinase and having the ability to hydrolyze model substrates including p-nitrophenyl butyrate (p-PNB), cutin, triacylglycerols (TAGs), and triolein (TO), yet their biological significance in pathogenesis stays subtle and uncharacterized. In a basic perspective, the measure of cutinase expressed by M. tuberculosis and part of these small lipolytic enzymes in the pathologic discipline require thorough characterization. So, through focusing on cutinase-encoding genes in M. tuberculosis and their active catalytic motif could help to build up a novel therapeutic approach.

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Designing fusion molecules from antigens of Mycobacterium tuberculosis for detection of multiple antibodies in plasma of TB patients

et al. 2020

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Heterogeneity among Homologs of Cutinase-Like Protein Cut5 in Mycobacteria

Cited by 6 publications

References 44 publications

Characterization of O‐mannosylated proteins profiling in bacillus Calmette–Guérin via gel‐based and gel‐free approaches

Characterization of O‐mannosylated proteins profiling in bacillus Calmette–Guérin via gel‐based and gel‐free approaches

Roles of Triolein and Lipolytic Protein in the Pathogenesis and Survival of Mycobacterium tuberculosis: a Novel Therapeutic Approach

Designing fusion molecules from antigens of Mycobacterium tuberculosis for detection of multiple antibodies in plasma of TB patients

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