Heterogeneity and lyophilization comparison of stool processing for gastrointestinal bile acid measurement by LC-MS/MS

Hu, Chenlin; Wang, Weiqun; Garey, Kevin W.

doi:10.1016/j.jchromb.2022.123569

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…At the same time, it is important to mention that lyophilization requires special equipment and elongates the sample processing time. The interchangeability of fresh and lyophilized feces samples was studied [153], demonstrating that the measured BA concentrations were comparable for original and lyophilized samples. Original, fresh feces can be a suitable matrix for BAs analysis except for in detailed investigations of trace bile acids.…”

Section: Matrices To Be Analyzed For Bile Acid Compositionmentioning

confidence: 88%

“…BAs are frequently measured from lyophilized feces instead of fresh ones [10,28,52,65,67,83,89,90,94,101,103,109,111,115,116,149,151,[153][154][155][156][157][158], thus expressing the BA concentrations based on the dry weight of the lyophilizate. At the same time, it is important to mention that lyophilization requires special equipment and elongates the sample processing time.…”

Section: Matrices To Be Analyzed For Bile Acid Compositionmentioning

confidence: 99%

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Determination of Bile Acids in Canine Biological Samples: Diagnostic Significance

Németh,

Sterczer,

Kiss

et al. 2024

Metabolites

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The comprehensive examination of bile acids is of paramount importance across various fields of health sciences, influencing physiology, microbiology, internal medicine, and pharmacology. While enzymatic reaction-based photometric methods remain fundamental for total BA measurements, there is a burgeoning demand for more sophisticated techniques such as liquid chromatography–tandem mass spectrometry (LC-MS/MS) for comprehensive BA profiling. This evolution reflects a need for nuanced diagnostic assessments in clinical practice. In canines, a BA assessment involves considering factors, such as food composition, transit times, and breed-specific variations. Multiple matrices, including blood, feces, urine, liver tissue, and gallbladder bile, offer insights into BA profiles, yet interpretations remain complex, particularly in fecal analysis due to sampling challenges and breed-specific differences. Despite ongoing efforts, a consensus regarding optimal matrices and diagnostic thresholds remains elusive, highlighting the need for further research. Emphasizing the scarcity of systematic animal studies and underscoring the importance of ap-propriate sampling methodologies, our review advocates for targeted investigations into BA alterations in canine pathology, promising insights into pathomechanisms, early disease detection, and therapeutic avenues.

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Section: Matrices To Be Analyzed For Bile Acid Compositionmentioning

confidence: 88%

Section: Matrices To Be Analyzed For Bile Acid Compositionmentioning

confidence: 99%

Determination of Bile Acids in Canine Biological Samples: Diagnostic Significance

Németh,

Sterczer,

Kiss

et al. 2024

Metabolites

View full text Add to dashboard Cite

show abstract

“…That study utilized lyophilized homogenized fecal samples and an untargeted metabolomic approach which is not optimized to detect BAs [ 169 ]. It is important to note that upstream sample handling can impact metabolite concentrations, and the process of lyophilization prior to targeted fecal BA metabolomics in humans was recently shown to increase BA concentrations by about two- to four-fold compared to non-lyophilized feces when then assessed via LC-MS/MS [ 170 ]. This thus highlights the importance of consistent methods of performing targeted metabolomics and the importance of understanding the employed methods.…”

Section: Feline Microbial-derived Bile Acidsmentioning

confidence: 99%

Collaborative Metabolism: Gut Microbes Play a Key Role in Canine and Feline Bile Acid Metabolism

Rowe,

Winston

2024

Veterinary Sciences

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Bile acids, produced by the liver and secreted into the gastrointestinal tract, are dynamic molecules capable of impacting the overall health of dogs and cats in many contexts. Importantly, the gut microbiota metabolizes host primary bile acids into chemically distinct secondary bile acids. This review explores the emergence of new literature connecting microbial-derived bile acid metabolism to canine and feline health and disease. Moreover, this review highlights multi-omic methodologies for translational research as an area for continued growth in veterinary medicine aimed at accelerating microbiome science and medicine as it pertains to bile acid metabolism in dogs and cats.

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Repurposing dried blood spot device technology to examine bile acid profiles in human dried fecal spot samples

Engevik,

Thapa,

Lillie

et al. 2024

American Journal of Physiology-Gastrointestinal and Liver Physi

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Dried blood spot (DBS) analysis has existed for >50 years, but application of this technique to fecal analysis remains limited. To address whether dried fecal spots (DFS) could be used to measure fecal bile acids, we collected feces from five subjects for each of the following cohorts: i) healthy individuals, ii) individuals with diarrhea, and iii) Clostridioides difficile-infected patients. Homogenized fecal extracts were loaded onto quantitative DBS (qDBS) devices, dried overnight, and shipped to the bioanalytical lab at ambient temperature. For comparison, source fecal extracts were shipped on dry ice and stored frozen. After four months, frozen fecal extracts and ambient DFS samples were processed and subjected to targeted LC-MS/MS-based metabolomics with stable isotope-labeled standards. We observed no differences in the bile acid levels measured between the traditional extraction and the qDBS-based DFS methods. This pilot data demonstrate that DFS-based analysis is feasible and warrants further development for fecal compounds and microbiome applications.

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Heterogeneity and lyophilization comparison of stool processing for gastrointestinal bile acid measurement by LC-MS/MS

Cited by 4 publications

References 21 publications

Determination of Bile Acids in Canine Biological Samples: Diagnostic Significance

Determination of Bile Acids in Canine Biological Samples: Diagnostic Significance

Collaborative Metabolism: Gut Microbes Play a Key Role in Canine and Feline Bile Acid Metabolism

Repurposing dried blood spot device technology to examine bile acid profiles in human dried fecal spot samples

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