The application of unmanned aerial vehicle (UAV) remote sensing technology for high-throughput acquisition of phenotypic values of field plant height is of great significance in plant height monitoring of wheat varieties (lines). Based on the UAV, mounted with high-resolution digital cameras, a low-altitude remote sensing platform was built to obtain images of the Berkut/Worrakatta recombinant inbred lines (RIL) wheat population (297 lines). The optimal digital surface model (DSM) for plant height extraction was constructed by combining the actual wheat plant heights measured in the field. Using a wheat 50K SNP chip, quantitative trait locus (QTL) analysis was performed for field plant height of the RIL population using UAV plant height data to detect the locus related to plant height. We verified the effectiveness of QTL analysis using the UAV plant height data. The results of the DSM plant height extraction method showed that the plant height extraction models during the entire growth period had a highly significant correlation (p < 0.001); the measured plant height correlated with the predicted plant height, with prediction model accuracy R2 = 0.7527 and verification model accuracy R2 = 0.8214. QTL analysis was conducted on the manually and UAV-measured plant height traits. For the manually measured plant height phenotypes, one locus related to plant height was detected on wheat chromosome 6A and explained 13.12% of phenotypic variation. For the UAV-measured phenotypes, one locus related to plant height was also detected on wheat chromosome 6A and explained 9.62% of phenotypic variation. The QPH.xjau-6A locus on chromosome 6A, which featured in the results of the two measurement methods, is a stable locus, indicating that the mapping results obtained using the actual plant height values were in good agreement with those obtained using the UAV extracted values. Three candidate genes related to plant height were screened: they encode protein kinase, NAC domain protein, and cytochrome P450, respectively. Therefore, this study provided reference information for monitoring plant phenotype and growth of wheat and also for the extraction of plant height for wheat breeding in the future.