Heterogeneity in Endothelial Cells with Special Reference to their Growth Related Proteins

Kumar, Shant; Kumar, Patricia; Pye, David; Abdul, Sattar; Wang, M.; Ponting, J

doi:10.1007/978-1-4615-3358-0_6

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…(GIBCO). Cultures of human brain-tumour-derived endothelial cells (BTEC) were established from a patient with medulloblastoma, as described (Kumar et al, 1992). Briefly, BTEC grew as a cobblestone monolayer and had a normal karyotype.…”

Section: Cell Culturesmentioning

confidence: 99%

Irradiation induces Up‐regulation of E9 protein (CD105) in human vascular endothelial cells

Wang

Kumar

Agthoven

et al. 1995

Intl Journal of Cancer

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The use of MAb E-9 raised against tissue-cultured endothelial cells (EC) has shown marked heterogeneity in vascular EC lining the blood vessels of normal and tumour tissues. MAb E-9 is human EC-specific and the protein recognized by it is a homodimer with a molecular mass of 97 kDa. The E-9 protein is resistant to treatment by 3 mM sodium periodate, but is sensitive to 10% trichloroacetic acid and 70% ethanol. E-9 protein has been assigned to a new cluster, CD105, and mapped to human chromosome 9q3. It has approximately 70% homology with type-III cell-surface receptor for transforming growth factor beta (TGF-beta). Recently CD105 has been reported to be the gene in patients with hereditary haemorrhagic telangiectasia. We have examined the effects of radiation on its expression in normal human umbilical-vein endothelial cells (HUVEC) and brain-tumour-derived endothelial cells (BTEC). Irradiation induced dose- and time-dependent up-regulation in the expression of the E-9 protein on the plasma membranes of EC, and also resulted in greater increase in the expression of the E-9 protein in semi-confluent (proliferating) as compared with confluent (non-proliferating) EC. It may well be that, following radiotherapy in cancer patients, E-9 protein is also up-regulated. The presence of increased amounts of E-9 protein in EC makes it an attractive target in the control of angiogenesis, especially after radiotherapy in cancer patients. The time scale involved in the up-regulation of E-9 protein following irradiation has led us to suggest that it may be a secondary event, the primary being the production and release of mitogenic factors (such as basic fibroblast growth factor) from irradiated EC.

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Section: Cell Culturesmentioning

confidence: 99%

Irradiation induces Up‐regulation of E9 protein (CD105) in human vascular endothelial cells

Wang

Kumar

Agthoven

et al. 1995

Intl Journal of Cancer

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A monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues

Wang

Kumar

Pye

et al. 1993

Intl Journal of Cancer

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103

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A new murine monoclonal antibody (MAb), E-9, has been raised using tissue-cultured human umbilical vein endothelial cells. The antigen recognized by this MAb is a peptide of 170 kDa under non-reducing conditions and 96 kDa under reducing conditions. MAb E-9 showed marked heterogeneity in its distribution in various tissues. The antigen recognized by it was present in vascular endothelial cells of all tumours, foetal organs and in regenerating and inflamed tissues. It stained a few normal tissues. However, with the exception of tonsils, staining tended to be weak and limited to a few blood vessels, as revealed by double staining using pan-endothelial antibody (CD31) and antibody to von Willebrand factor, another marker of vascular endothelium. Surprisingly, blood vessels within the placental villi were completely negative. The function of the antigen recognized by MAb E-9 is not known, but its evaluation and use should increase our understanding of angiogenesis.

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Heterogeneity in Endothelial Cells with Special Reference to their Growth Related Proteins

Cited by 2 publications

References 22 publications

Irradiation induces Up‐regulation of E9 protein (CD105) in human vascular endothelial cells

Irradiation induces Up‐regulation of E9 protein (CD105) in human vascular endothelial cells

A monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues

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