Heterogeneity in single-cell outgrowth of Listeria monocytogenes in half Fraser enrichment broth is affected by strain variability and physiological state

Bannenberg, Jasper W.; Tempelaars, Marcel H.; Zwietering, Marcel H.; Abee, Tjakko; Besten, H.M.W. den

doi:10.1016/j.foodres.2021.110783

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…Half-Fraser, including the supplement with half the concentration of acriflavine and nalidixic acid, is recommended as a first stage for recovery of stressed or damaged Listeria spp. while suppressing undesired microorganisms [78,79,82]. For a second enrichment, a 0.1 mL inoculum is transferred to 10 mL of Fraser broth, containing double the concentration of selective compounds, and incubated at 24 h ± 2 at 37 • C.…”

Section: Microbiological Testing Of Listeria Spp In the Food Industrymentioning

confidence: 99%

An Exploration of Listeria monocytogenes, Its Influence on the UK Food Industry and Future Public Health Strategies

Macleod

Beeton

Blaxland

2022

Foods

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Listeria monocytogenes is a Gram-positive intracellular pathogen that can cause listeriosis, an invasive disease affecting pregnant women, neonates, the elderly, and immunocompromised individuals. Principally foodborne, the pathogen is transmitted typically through contaminated foods. As a result, food manufacturers exert considerable efforts to eliminate L. monocytogenes from foodstuffs and the environment through food processing and disinfection. However, L. monocytogenes demonstrates a range of environmental stress tolerances, resulting in persistent colonies that act as reservoirs for the reintroduction of L. monocytogenes to food contact surfaces and food. Novel technologies for the rapid detection of L. monocytogenes and disinfection of food manufacturing industries have been developed to overcome these obstacles to minimise the risk of outbreaks and sporadic cases of listeriosis. This review is aimed at exploring L. monocytogenes in the UK, providing a summary of outbreaks, current routine microbiological testing and the increasing awareness of biocide tolerances. Recommendations for future research in the UK are made, pertaining to expanding the understanding of L. monocytogenes dissemination in the UK food industry and the continuation of novel technological developments for disinfection of food and the food manufacturing environment

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Section: Microbiological Testing Of Listeria Spp In the Food Industrymentioning

confidence: 99%

An Exploration of Listeria monocytogenes, Its Influence on the UK Food Industry and Future Public Health Strategies

Macleod

Beeton

Blaxland

2022

Foods

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“…The plates were then incubated microaerobically at 41.5 °C for 48 h and samples were taken throughout enrichment. Preferably, the growth of each cell would be observed General discussion 201 continuously through optical density (OD600) measurements in a spectrophotometer, as was done for Listeria monocytogenes in a similar setup (Bannenberg et al, 2021).…”

Section: Intra-species Heterogeneitymentioning

confidence: 99%

Rapid and reliable detection of foodborne pathogens : Exploring Campylobacter ecology in enrichments to improve detection

Lanzl¹

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In 1988, Black et al. conducted a trial, in which they administered adult volunteers with food artificially contaminated with different concentrations of two C. jejuni strains to establish a dose-response relation for Campylobacter infections from food . They found not only that one of the strains was more likely to cause illness and that illnesses were more severe, but they also concluded that already 800 cells were sufficient to cause campylobacteriosis . Later, mathematical models and microbiological risk assessments were used to estimate a dose-response relation. Medema et al. (1996) suggested that the ingested dose resulting in illness was 9•10 4 cells but also mentioned that the risk differed between C. jejuni isolates. It is well established that C. jejuni is responsible for most foodborne Campylobacter infections, but C. coli has been recognized to be responsible for a fourth of all campylobacteriosis cases (Gürtler et al., 2005;Inglis et al., 2011) and symptoms are similar to those induced by C. jejuni . It is important to mention that, although it has been suggested that the infectious dose of C. jejuni is much lower than other foodborne pathogens (Kothary and Babu, 2001), no clear dose-response relation can be established as it is not only dependent on the pathogenicity of the strain but on other factors like the health state and demographics of the individuals ingesting the Campylobacter cells. Although everyone can be infected by Campylobacter, infections are predominantly common in certain age groups; in industrialized countries, the incidence rate is generally higher in young children until the age of four (

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Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions

Souza

Bergis

et al. 2023

Food Microbiology

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Heterogeneity in single-cell outgrowth of Listeria monocytogenes in half Fraser enrichment broth is affected by strain variability and physiological state

Cited by 8 publications

References 17 publications

An Exploration of Listeria monocytogenes, Its Influence on the UK Food Industry and Future Public Health Strategies

An Exploration of Listeria monocytogenes, Its Influence on the UK Food Industry and Future Public Health Strategies

Rapid and reliable detection of foodborne pathogens : Exploring Campylobacter ecology in enrichments to improve detection

Assessment of the relationship between the MLST genetic diversity of Listeria monocytogenes and growth under selective and non-selective conditions

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