Heterogeneity of Chick Embryo Cells with regard to Newcastle Disease Virus Multiplication

Abstract

SUMMARYNewcastle disease virus (NDV) production by individual chick embryo cells was determined after infection of monolayer cultures, trypsinization and distribution of the ceils into a large number of test tubes so that each tube would contain an average of one cell (or in some experiments IO cells). The content of each tube was assayed for p.f.u. I8 h later. The statistical analysis of the results indicates the presence of at least three distinct populations of cells with regard to NDV production: one non-producing population (5o to 90 ~o of the cells); one low-producing population (80 ~o of the producing cells) and one high-producing (20 ~ of the producing cells).The relative proportions of these cell populations was the same whether primary, secondary or tertiary cultures were used, and also when the cell-cultures were obtained from different tissues of the chick embryo.

Taming the Trojan horse: optimizing dynamic carrier cell/oncolytic virus systems for cancer biotherapy

“…Nonetheless such analysis is possible using basic virological techniques, and has indeed been used in the past to illuminate subpopulations of cultured cells with marked differences in viral productivity. 28 Cell to cell differences in viral productivity would have an impact on their utility as carriers in vivo, as each is likely to behave as an independent virus factory following Optimizing dynamic CC/OV systems AT Power and JC Bell delivery to a particular region of the tumor. Depending on the source of any such heterogeneity, measures such as cell cycle synchronization or fluorescence-activated cell sorting might be used to ensure that each CC administered is capable of maximal virus production upon arrival at the tumor.…”

Replication Cycle of Newcastle Disease Virus in Three Host Cells of Different Permissiveness

“…However, according to the origin of the cells, a large variation in virus production may be observed (Wilcox, 1959;Hecht & Summers, I974;Huppert et al I974a). Surprisingly, chicken embryo cells (CEC) in primary or secondary cultures are not very efficient NDV producers, yielding only 2 to 2o p.f.u./cell.…”