Heterogeneity of chicken slow skeletal muscle troponin T mRNA

Yonemura, Izuru; Hirabayashi, Tamio; Miyazaki, Junichi

doi:10.1002/(sici)1097-010x(20000201)286:2<149::aid-jez7>3.0.co;2-h

Cited by 14 publications

(21 citation statements)

References 29 publications

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“…As has been previously reported (Campinho et al, 2005) and in contrast to what occurs in tetrapods, sea bream sTnT genes have a coordinated expression pattern during sea bream development (Barton et al, 1999;Briggs and Schachat, 1993;Bucher et al, 1999;Farza et al, 1998;Gahlmann et al, 1987;Huang et al, 1999;Jin et al, 1998a;Jin et al, 2000;Perry, 1998;Samson et al, 1994;Wang and Jin, 1997;Wang et al, 2002;Yonemura et al, 2000;Yonemura et al, 2002). Gene expression of sTnT1sb (Fig.·7) and its splice variants in sea bream, but not sTnT2sb and iTnT (Fig.·6), are affected by T3 treatment.…”

Section: Sea Bream Tnt Genes Th Responsivenessmentioning

confidence: 52%

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

Campinho

Sweeney

Power

2006

Journal of Experimental Biology

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SUMMARY In the sea bream Sparus auratus three stage-specific fast troponin T (fTnT) isoforms have been cloned and correspond to embryonic-, larval- and adult-specific isoforms. Characterisation, using database searches, of the putative genomic organisation of Fugu rubripes and Tetraodon nigroviridis fTnT indicates that alternative exon splicing in the 5 region of the gene generates the different isoforms. Moreover, comparison of teleost fTnTs suggests that alternative splicing of fTnT appears to be common in teleosts. A different temporal expression pattern for each fTnT splice varotnt is found during sea bream development and probably relates to differing functional demands, as a highly acidic embryonic form (pI 5.16) is substituted by a basic larval form (pI 9.57). Thyroid hormones (THs), which play an important regulatory role in muscle development in flatfish and tetrapods,appear also to influence TnT gene expression in the sea bream. However, THs have a divergent action on different sea bream TnT genes and although the slow isoform (sTnT1) is TH-responsive, fTnT, sTnT2 and the itronless isoform (iTnT) are unaffected. The present results taken together with those published for flatfish seem to suggest differences may exist in the regulation of larval muscle development in teleosts.

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Section: Sea Bream Tnt Genes Th Responsivenessmentioning

confidence: 52%

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

Campinho

Sweeney

Power

2006

Journal of Experimental Biology

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“…5A). The band of 690 nt corresponds with the transcript lacking the codon for alanine, and the bands of 558 nt and about 440 nt with the transcript containing the codon for alanine (Yonemura et al, 2000). No difference in the expression patterns of sTnT transcripts was found during development.…”

Section: Heterogeneity In Stnt Mrnamentioning

confidence: 89%

“…Using chicken sTnT cDNA clones (Yonemura et al ., 1996), the probe for S1 nuclease mapping, namely probe A, was constructed in the previous study (Yonemura et al ., 2000). The probe A, labeled with [ 32 P] ATP using T4 polynucleotide kinase (Nippon gene), is a 690 nt fragment (position 1 to 690 of chicken sTnT cDNA) lacking three bases (135-137) for alanine.…”

Section: Antisense Dna Probes and S1 Nuclease Mappingmentioning

confidence: 99%

“…Our previous study showed the heterogeneity in the 5'-region of sTnT transcripts which lead to three amino acid changes in sTnT proteins (Yonemura et al, 2000). Typical slow skeletal muscle, ALD, had two kinds of transcripts, one having the codon for alanine (position 135-137), C (258), and A (262) and the other lacking the codon and having T (258) and G (262) instead of C and A.…”

Section: Heterogeneity In Stnt Mrnamentioning

confidence: 99%

“…(2000) found variants of chicken sTnT cDNA, which differed from one another by inclusion or exclusion of one codon encoding an alanine residue and single base substitutions at two positions in the 5'-region and at four positions in the 3'-region. The heterogeneity of chicken sTnT transcripts is generated from 3.5 kb of the sTnT gene (Yonemura et al ., 2000), although the chicken fTnT gene is about 35 kb (Miyazaki et al ., 1999) and the chicken cTnT gene is about 9 kb (Cooper and Ordahl, 1985). Only two kinds of sTnT isoforms were detected in the adult chicken slow muscle, anterior latissimus dorsi (ALD) (Mikawa et al ., 1981).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Developmental Changes of Cardiac and Slow Skeletal Muscle Troponin T Expression in Chicken Cardiac and Skeletal Muscles

et al. 2002

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Comparative studies on the expression patterns of three troponin T genes during mouse development

et al. 2001

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In vertebrates, three troponin T (TnT) genes, cardiac TnT (cTnT), skeletal muscle fast-twitch TnT (fTnT), and slow-twitch TnT (sTnT), have evolved for the regulation of striated muscle contraction. To understand the mechanism for muscle fiber-specific expression of the TnT genes, we compared their expression patterns during mouse development. Our data revealed that the TnT expression in the developing embryo was not as restricted as that in the adult. In addition to a strong expression in the developing heart beginning at day 7.5 p.c (postcoitum), the cTnT transcript was detected at later stages in some skeletal muscles, where beginning at day 11.75 p.c. the fTnT and sTnT genes were also expressed. Only sTnT but not fTnT was found transiently in the developing heart. At day 13.5 p.c., expressions of all three genes were detected in the developing tongue and this co-expression continued to day 16.5 p.c. with the fTnT isoform being predominant. At this stage, overlapping and distinct expression patterns of both sTnT and fTnT genes were also evident in many developing skeletal muscles. These data suggest that different muscles during development undergo a complex change in TnT isoforms resulting in different contractile properties. Unexpectedly, the cTnT transcript was persistently found in the developing bladder, where presumably smooth muscle is present. In transgenic mice, expression of a LacZ gene driven by a rat cTnT promoter (Ϫ497 to ϩ192 bp) was very similar to that of the endogenous cTnT gene, suggesting that this promoter contained regulatory elements sufficient for the control of tissue-specific cTnT expression during development. Anat Rec 263: [72][73][74][75][76][77][78][79][80][81][82][83][84] 2001.

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Heterogeneity of chicken slow skeletal muscle troponin T mRNA

Cited by 14 publications

References 29 publications

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

Developmental Changes of Cardiac and Slow Skeletal Muscle Troponin T Expression in Chicken Cardiac and Skeletal Muscles

Comparative studies on the expression patterns of three troponin T genes during mouse development

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