Heterogeneity of Fcγ receptor-bearing cells in human term amniochorion

Bright, Nicholas A.; Ockleford, Colin

doi:10.1016/0143-4004(94)90016-7

Cited by 14 publications

(3 citation statements)

References 14 publications

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“…This potential route of transmission is supported by the detection of ZIKV in amniotic fluid 27 . Little is known about the expression of Fc receptors at the amniochorionic membrane itself, but cells in the decidual layer associated with the amniochorionic membrane express Fcγ receptors and therefore could support ADE 28 . Based on our results, infection and ADE in the paraplacental compartment seems likely, but less likely than in the placental compartment.…”

Section: Discussionmentioning

confidence: 99%

Zika virus infection in human placental tissue explants is enhanced in the presence of dengue virus antibodies in-vitro

Hermanns

Göhner

Kopp

et al. 2018

Emerging Microbes & Infections

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The current Zika virus (ZIKV) outbreak is associated with neurological malformations and disorders in neonates. Areas of increased incidence of malformations may overlap with dengue-hyperendemic areas. ZIKV infection is enhanced by antibodies against dengue virus (DENV) in cell culture and inbred mice. Sufficiently powered clinical studies or primate studies addressing the enhancement of fetal ZIKV infection after previous dengue infection are not available. The human placenta is susceptible to ZIKV in vitro, but it is unknown whether antibody-dependent enhancement of ZIKV infection occurs at the placental barrier. Here we studied ZIKV infection in placental tissue in the presence of DENV-immune sera. Explants from the amniochorionic membrane, the chorionic villi, and the maternal decidua were infected with ZIKV in the presence of DENV type 1-, 2-, or 4-immune sera, or controls. Presence of DENV antibodies of any type enhanced the percentage of successful infections of organ explants between 1.42- and 2.67-fold, and led to a faster replication as well as significantly increased virus production. No enhancement was seen with yellow fever or chikungunya virus control sera. Pre-existing DENV antibodies may pose an increased risk of trans-placental ZIKV transmission.

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Section: Discussionmentioning

confidence: 99%

Zika virus infection in human placental tissue explants is enhanced in the presence of dengue virus antibodies in-vitro

Hermanns

Göhner

Kopp

et al. 2018

Emerging Microbes & Infections

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“…Passive immunity is provided to the human fetus by transfer of maternal IgG into the fetal circulation. This can occur either via the amniotic fluid and fetal gut -a pathway that is considered insignificant -or via the vascular system of the placenta [1]. Consequently, the syncytiotrophoblast layer that covers the placental chorionic villi plays a key role in IgG transport.…”

Section: Introductionmentioning

confidence: 99%

IgG transport across trophoblast-derived BeWo cells: a model system to study IgG transport in the placenta

et al. 1999

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In primates, prenatal transfer of IgG from mother to offspring occurs predominantly across the placenta. Although a number of Fcgamma-receptors and IgG binding proteins have been detected in human placental tissue, an involvement of any of these receptors in IgG transport across the syncytiotrophoblast remains to be demonstrated. Therefore, we investigated the mechanism of IgG transcytosis in trophoblast-derived BeWo cells. BeWo cells were not only found to express the MHC class I-related IgG Fc receptor, human FcRn, but also specifically bound fluorescein isothiocyanate (FITC)-labeled human IgG (FITC-hIgG) at the apical surface at mildly acidic pH. The cells preferentially transcytosed FITC-hIgG from the apical to the basolateral side when compared to the fluid-phase marker FITC-dextran and to FITC-hIgG transcytosis in the opposite direction. However, endocytosis of FITC-hIgG at the apical plasma membrane at physiological pH required the continuous presence of FITC-hIgG at concentrations similar to those present in the maternal circulation. These results suggest a mechanism by which IgG is internalized by BeWo cells via fluid-phase endocytosis. Tight binding of IgG to hFcRn may then occur in acidic endosomes, followed by selective sorting into the transcytotic pathway. Thus, the main function of this receptor is to prevent entry of IgG into the degradative pathway in lysosomes.

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“…In vitro studies, using BSA/anti-BSA rabbit IgG complexes (Frey et al 1984), suggested expression of FcgR on human fibroblasts. All three classes of FcgR were found to be expressed on fibroblasts in reticular layers in placenta (Bright & Ockleford 1994). We observed spindle-shaped FcgRI π and FcgRII π cells in the fiber-rich area in zones B and C, which might be fibroblasts.…”

Section: Fcgrii-mediated Phagocytosis Bymentioning

confidence: 58%

Topical distribution of FcgRI, FcgRII and FcgRIII in inflamed human gingiva

Yuan

Schreurs²,

Gjermo

et al. 1999

J Clinic Periodontology

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The topical distribution of Fc gamma receptor types I, II and III (Fc gammaRI-III) was analyzed by means of immunohistochemistry in human gingival tissue obtained from 12 patients with chronic periodontitis. CD68+ macrophages expressing all three classes of Fc gammaR were found throughout the whole gingival connective tissue (CT), whereas dense infiltrates of polymorphonuclear granulocytes (identified by staining for neutrophil elastase) with strong staining for Fc gammaRIII and Fc gammaRII were found subjacent to the apical part of the pocket epithelium (PE) and in the PE itself. CD19+ B lymphocytes with variable staining intensity for Fc gammaRII were observed in clusters subjacent to the PE and extending into the central part of the CT. Only a few scattered CD3+ T lymphocytes stained for Fc gammaRIII. Some spindle-shaped cells (CD68-, therefore non-macrophages) and apparently non-cellular fibrous tissue elements stained for Fc gammaRI and Fc gammaRII. In the epithelium, Fc gammaRII+ dendritic cells were frequently observed in the entire oral gingival epithelium and in the coronal part of the PE. Occasionally, some keratinocytes which stained for Fc gammaRII and Fc gammaRIII were found. The observations indicate that Fc gammaR of the various classes are amply expressed on numerous cell types in inflamed gingival tissue. The specific distribution pattern detected suggests that Fc gammaRs may play a role in the mediation of chronic inflammation in the periodontal lesion.

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Heterogeneity of Fcγ receptor-bearing cells in human term amniochorion

Cited by 14 publications

References 14 publications

Zika virus infection in human placental tissue explants is enhanced in the presence of dengue virus antibodies in-vitro

Zika virus infection in human placental tissue explants is enhanced in the presence of dengue virus antibodies in-vitro

IgG transport across trophoblast-derived BeWo cells: a model system to study IgG transport in the placenta

Topical distribution of FcgRI, FcgRII and FcgRIII in inflamed human gingiva

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