Heterogeneity of Genotype-Phenotype Correlation among Macrolide-Resistant
            <i>Streptococcus agalactiae</i>
            Isolates

Both, Ulrich von; Buerckstuemmer, Anna; Fluegge, Kirsten; Berner, Reinhard

doi:10.1128/aac.49.7.3080-3082.2005

Cited by 12 publications

(12 citation statements)

References 19 publications

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“…It is particularly prudent to discover virulence factors in serotype V isolates, as they have often been reported to be resistant to antibiotics [12,26,[33][34][35]. It is particularly prudent to discover virulence factors in serotype V isolates, as they have often been reported to be resistant to antibiotics [12,26,[33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Distribution of novel and previously investigated virulence genes in colonizing and invasive isolates ofStreptococcus agalactiae

et al. 2006

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Although Streptococcus agalactiae has emerged as an important cause of invasive disease, relatively little is known regarding the genetic basis of virulence of this organism. Three novel genes with characteristics suggesting a role in virulence were identified via comparison of sequenced genomes of S. agalactiae. The presence of these genes and of the previously identified genes bac, bca, rib, and spb1 was determined, and isolates were assigned a binary genetic signature. It was found that isolates containing spb1, previously suggested to be limited to serotype III-3, were represented by 18 different genetic signatures and several serotypes, and that the presence of both sbp1 and rib was more predictive of invasive disease than spb1 alone. Additionally, bac-positive isolates, reported to be genetically homogeneous, were represented by 14 different genetic signatures. Finally, the majority of serotype V isolates examined contained zero or only one of the genes tested, suggesting that much remains undiscovered regarding important virulence factors in isolates of this serotype.

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Section: Discussionmentioning

confidence: 99%

Distribution of novel and previously investigated virulence genes in colonizing and invasive isolates ofStreptococcus agalactiae

et al. 2006

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“…The efflux pump is encoded by the mefA gene, whereas the acquisition of ermB and/or ermTR (erythromycin ribosome methylase) genes confers a ribosomal modification. erm genes are associated with macrolide-lincosamide-streptogramin B (MLS B ) resistance and can be expressed constitutively (cMLS B ) or upon induction (iMLS B ), although there is a heterogenic distribution of erm genes among these phenotypes [39]. Several studies have indicated how macrolide resistance might be acquired.…”

Section: Macrolide Resistancementioning

confidence: 99%

“…High rates of erythromycin and clindamycin resistance have, however, only been rarely reported: in one study testing 200 GBS isolates collected from vaginal/ rectal specimens [38], the resistance rate was 54% and 33%, respectively. Among erythromycin-resistant isolates, several studies have reported serotype V as the most frequent serotype [39][40][41][42][43].…”

Section: Macrolide-and Clindamycin-resistant Strainsmentioning

confidence: 99%

Invasive Group B Streptococcal Disease in Non-pregnant Adults

2008

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Streptococcus agalactiae, commonly referred as group B Streptococcus (GBS), is a major cause of neonatal sepsis and infections in pregnant women. However, the number of invasive infections in non-pregnant adults is growing. Elderly patients and those with chronic underlying conditions, such as diabetes mellitus or compromised immune defence, are at increased risk of invasion. The spectrum of clinical manifestations is broad and includes necrotizing fasciitis and toxic shock syndrome. Although, primary bacteremia and skin and soft-tissue infections are the most frequently reported diagnosis. This article reviews the epidemiology, pathogenesis and treatment of invasive GBS disease in non-pregnant adults, with an emphasis on skin and soft-tissue infections.

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“…[4,5] To treat GBS infection, penicillin, ampicillin, and cefazolin are the drugs of choice; for patients allergic to penicillin or cephalosporins, vancomycin, macrolides (such as erythromycin, azithromycin, and clarithromycin), and lincosamides (clindamycin) may be used as the alternative drugs. [6][7][8][9] Vancomycin resistance has not been reported in GBS. However, in the USA, 12% of pregnant women are allergic to penicillin, and 25% and 7% of GBS isolates from these women are resistant to erythromycin and clindamycin, respectively.…”

Section: S Treptococcus Agalactiae (Group B Streptococcus Gbs)mentioning

confidence: 99%

Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan

Wang¹,

Chen²,

Hou³

et al. 2015

Biomed J

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Background: Antimicrobial resistance of Streptococcus agalactiae (Group B Streptococcus, GBS) has been emerging worldwide. We aimed to examine the correlation of drug-resistant genes with serotypes and with the mutations of the quinolone resistance-determining region (QRDR) in GBS isolates. Methods:A total of 323 human GBS isolates were collected from a hospital in southern Taiwan. Laboratory investigation included serotyping by a multiplex polymerase chain reaction (PCR) method, antimicrobial susceptibility testing by a disc diffusion method, and mechanism analysis of the resistance to macrolides and fluoroquinolones by PCR and sequencing methods. Results:Multiplex PCR showed that the most prevalent serotypes were Ib, III, V, and VI, mostly isolated from urine. The ermB gene was highly prevalent in serotypes Ib and V and was associated with clindamycin and macrolide resistance. GBS with a serine-to-leucine mutation at codon 81 in GyrA and with a serine-to-phenylalanine or -tyrosine mutation at codon 79 in ParC had a higher minimum inhibitory concentration of levofloxacin than isolates with only an aspartic acid-to-tyrosine mutation at codon 83 (>32 μg/ml vs. 16 μg/ml) in GyrA. Conclusions:The most prevalent GBS serotypes were Ib, III, V, and VI. The ermB and mefE genes carried in serotypes Ib and V were highly associated with the resistance to macrolides and clindamycin. Mutations at codon 79 and codon 83 of ParC were the major determining factors for high-level fluoroquinolone resistance. (Biomed J 2015;38:215-220)

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Heterogeneity of Genotype-Phenotype Correlation among Macrolide-Resistant Streptococcus agalactiae Isolates

Cited by 12 publications

References 19 publications

Distribution of novel and previously investigated virulence genes in colonizing and invasive isolates ofStreptococcus agalactiae

Distribution of novel and previously investigated virulence genes in colonizing and invasive isolates ofStreptococcus agalactiae

Invasive Group B Streptococcal Disease in Non-pregnant Adults

Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan

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