Heterogeneity of horse spleen ferritin and apoferritin: Comparison of electrophoretic and chromatographic fractions

Suran, Anita A.; Tarver, Harold

doi:10.1016/0003-9861(65)90202-x

Cited by 47 publications

(30 citation statements)

References 20 publications

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“…Phycomyces and horse spleen ferritins were resolved into three bands through the use of polyacrylamide disc gel electrophoresis. These bands have previously been shown to represent monomers and oligomers of both mammalian (12,18,28,29) and fungal ferritin (4). The number of bands obtained from ferritin preparations is dependent upon the source of the ferritin and the concentration of the applied sample (2,15,17,22).…”

Section: Discussionmentioning

confidence: 96%

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Isolation and characterization of Phycomyces blakesleeanus ferritin

LaBombardi¹,

Pisano²,

Klavins³

1982

J Bacteriol

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Ferritin was isolated from the fungus Phycomyces blakesleeanus and compared biochemically and immunologically with horse spleen ferritin. Phycomyces and horse spleen ferritins were shown to exhibit similar electrophoretic patterns on polyacrylamide gels. Both preparations yielded an identical single band on sodium dodecyl sulfate-containing polyacrylamide gels. Tryptic digests of Phycomyces ferritin yielded 17 ninhydrin-positive spots as compared to 26 for horse spleen ferritin tryptic digests. Phycomyces ferritin was immunologically unrelated to horse spleen ferritin.

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Section: Discussionmentioning

confidence: 96%

“…When 'purified ferritin is subjected to polyacrylamide gel electrophoresis, multiple bands are observed. These bands have been shown to represent monomers, dimers, and oligomers of ferritin (12,18,28,29). The iron content of ferritin is not responsible for this phenomenon because ferritin and apoferritin have identical mobilities (27).…”

mentioning

confidence: 99%

Isolation and characterization of Phycomyces blakesleeanus ferritin

LaBombardi¹,

Pisano²,

Klavins³

1982

J Bacteriol

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“…Several other groups have also observed a heterogeneity in ferritins by ion-exchange chromatography. Theron et al (1963) and Suran & Tarver (1965) obtained three fractions from horse spleen ferritin by DEAE-cellulose chromatography. Yu & Fineberg (1965) obtained three chromatographic fractions from rat liver that could be distinguished electrophoretically.…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity in tissue ferritins displayed by gel electrofocusing

Drysdale

1974

Biochemical Journal

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1. Horse spleen ferritin and human liver ferritin were examined by gel electrofocusing under conditions that demonstrated equilibrium focusing. Both ferritins were resolved into multiple isoferritins. Both families of isoferritins were separable from one another. 2. Horse spleen ferritin was also resolved into five components by ion-exchange chromatography on DEAE-Sephadex A-50. Each of the major chromatographic fractions contained only a few of the isoferritins seen on gel electrofocusing. Each chromatographic fraction corresponded to different portions of the isoferritin profile. 3. These results indicate that the heterogeneity seen in many ferritins by gel electrofocusing represents structural heterogeneity in the ferritin population as isolated from the tissues.

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“…The suggestion of an H-to-L-subunit conversion enhanced by iron is an interesting one with respect to structure-function relationships. The Nterminal amino acid (serine) in horse spleen ferritin is acetylated (Suran & Tarver, 1965;Harrison et al, 1962). Huberman & Franco (1975) report the isolation of N-acetylseryl-tRNA from rat liver and find its concentration to be increased in iron-stimulated rats.…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity in horse ferritins. A comparative study of surface charge, iron content and kinetics of iron uptake

Russell¹,

Harrison²

1978

Biochemical Journal

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Horse ferritins from different organs show heterogeneity on electrofocusing in Ampholine gradients. Both ferritin and apoferritin from liver and spleen could be fractionated with respect to surface charge by serial precipitation with (NHI4)2SO4. In the ferritin fractions, increasing iron content parallels increasing isoelectric point. After removal of their iron those fractions which originally contained most iron accumulated added iron at the fastest rates. When unfractionated ferritins from different organs were compared the average isoelectric point increased in order spleen< liver< kidney< heart. The order of initial rates of iron uptake by the apoferritins was spleen> kidney> heart and initial average iron contents also followed this order. The relatively low rates of iron accumulation by ironpoor molecules may have been due to structural alteration, to degradation, to activation of the iron-rich molecules or to other factors.

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Heterogeneity of horse spleen ferritin and apoferritin: Comparison of electrophoretic and chromatographic fractions

Cited by 47 publications

References 20 publications

Isolation and characterization of Phycomyces blakesleeanus ferritin

Isolation and characterization of Phycomyces blakesleeanus ferritin

Heterogeneity in tissue ferritins displayed by gel electrofocusing

Heterogeneity in horse ferritins. A comparative study of surface charge, iron content and kinetics of iron uptake

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