Heterogeneity of lipoprotein B

Cham, Bill E.; Owens, P. C.; Roeser, H. P.; Gaffney, T. J.; Shanley, B. C.

doi:10.1016/0006-291x(81)91679-x

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…It is known that plasma, delipidated with a butanol-DIPE mixture, retains apolipoproteins in solution [1][2][3][21][22][23][24][25][26][27][28][29]. In a recent study we have shown that reinfusion in normocholesterolemic animals [ 111.…”

Section: Discussionmentioning

confidence: 99%

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Cham

Kostner²,

Dwivedy³

et al. 1996

J. Clin. Apheresis

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Lipid apheresis, a new extracorporeal procedure based on plasma delipidation and showing promise as a possible treatment for atherosclerosis, was recently reported for the first time from this laboratory [Cham et al., J Clin Apheresis 10:61–69. 1995]. In the present study lipid apheresis was applied to hypercholesterolemic and normocholesterolemic roosters to examine its effect on plasma lipoprotein particles. This procedure resulted in conspicuous changes in electrophoretic patterns of plasma lipoproteins. The electrophoretic mobilities of all the lipoprotein fractions had changed considerably. Lipid stainable material was present in at least three bands in the α‐globulin area. In particular, changes in the electrophoretic region of high‐density lipoproteins were observed. Lipid apheresis markedly induced the anti‐atherogenic pre‐β‐high‐density lipoproteins. The observed changes induced by lipid apheresis were more pronounced in the hyperlipidemic animals compared with the normocholesterolemic controls. A novel pre‐α‐lipoprotein band was observed soon after lipid apheresis. This lipoprotein band had a density larger than 1.21. At approximately 150 minutes after lipid apheresis, the electrophoretic pattern had almost returned to its original base pattern. Lipid apheresis results in plasma lipoprotein changes which may induce reverse cholesterol transport and shows promise as a possible treatment of atherosclerosis. © 1996 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Cham

Kostner²,

Dwivedy³

et al. 1996

J. Clin. Apheresis

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“…Each of these lipoprotein fractions is heterogeneous regarding their lipid and protein contents. Even a narrow LDL fraction with a density of 1.027 -1.050, initially considered as lipoprotein B consisting reputedly of only ApoB, was subsequently shown to be heterogeneous in its protein contents as shown with ultracentrifugation, gel permeation chromatography, polyacrylamide gel electrophoresis and isoelectric focusing-studies [6].…”

Section: Atherogenic Lipoproteinsmentioning

confidence: 99%

Intravascular infusion of autologous delipidated plasma induces antiatherogenic lipoproteins and causes regression of atherosclerosis <br>—Studies in non-primates, monkeys and humans

Cham¹,

Chase²

2013

Health

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Atherosclerosis is the primary pathophysiological cause of heart disease and cerebrovascular disease. It is responsible for more than 20% of deaths worldwide each year. Treatments for atherosclerosis may include lifestyle changes, drugs, and medical procedures or surgery. There is a need for a rapid and effective treatment for this disease. In 1976, it was hypothesized that a multifunctional plasma delipidation process when applied to hyperlipidemic patients would lead to rapid regression of atherosclerosis. The procedure has now been applied to a variety of nonprimates, primates and humans. In all models studied, large quantities of antiatherogenic lipoprotein particles were generated that led to the mechanisms of reverse cholesterol transport. Trends to regression and actual regression of atherosclerosis have now been reported using a specific plasma delipidation process consisting of lipid extraction from plasma with mixtures of butanol and ethers.

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Lipid apheresis: An in vivo application of plasma delipidation with organic solvents resulting in acute transient reduction of circulating plasma lipids in animals

Cham

Kostner

Dwivedy

et al. 1995

J of Clinical Apheresis

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Despite primary and secondary prevention of coronary disease with lowering plasma cholesterol by diet and drug therapy, coronary heart disease remains the major cause of death in Western countries. Low density lipoprotein apheresis had the potential to make a significant impact as it acutely leads to a marked reduction in plasma cholesterol. However, recent preliminary results suggest that low density lipoprotein apheresis may not be more effective in preventing progression of coronary disease than current drug therapy. We have devised a new technique, termed lipid apheresis, which removes cholesterol and triglycerides from plasma but retains the apolipoproteins. This procedure shows great promise in stimulating regression beyond current therapy. Lipid apheresis, a new extracorporeal procedure based on plasma delipidation with the organic solvent mixture butanol-diisopropyl ether, was applied to hypercholesterolemic and normocholesterolemic roosters. Approximately 25% of the calculated blood volume was removed from the animals. The plasma was separated from the blood cells. The plasma was delipidated for 20 min with the organic solvent mixture. The delipidated plasma containing all proteins, including the apolipoproteins and other ionic constituents, was remixed with the blood cells and infused back into the identical donor animals. Analyses of serial blood samples collected from lipid apheresed and sham treated animals up to 16 h after infusion revealed that lipid apheresis caused acute, marked reductions in plasma lipids. The pattern and extent of the plasma levels of cholesterol were different in the hypercholesterolemic animals when compared with normocholesterolemic animals, indicating that a readily extraplasma cholesterol pool in the hypercholesterolemic animals was rapidly mobilized into the plasma pool.(ABSTRACT TRUNCATED AT 250 WORDS)

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Heterogeneity of lipoprotein B

Cited by 4 publications

References 30 publications

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Intravascular infusion of autologous delipidated plasma induces antiatherogenic lipoproteins and causes regression of atherosclerosis <br>—Studies in non-primates, monkeys and humans

Lipid apheresis: An in vivo application of plasma delipidation with organic solvents resulting in acute transient reduction of circulating plasma lipids in animals

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Heterogeneity of lipoprotein B

Cited by 4 publications

References 30 publications

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Intravascular infusion of autologous delipidated plasma induces antiatherogenic lipoproteins and causes regression of atherosclerosis &lt;br&gt;—Studies in non-primates, monkeys and humans

Lipid apheresis: An in vivo application of plasma delipidation with organic solvents resulting in acute transient reduction of circulating plasma lipids in animals

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Intravascular infusion of autologous delipidated plasma induces antiatherogenic lipoproteins and causes regression of atherosclerosis <br>—Studies in non-primates, monkeys and humans