Heterogeneity of milk fat globule membrane structure and composition as observed using fluorescence microscopy techniques

Evers, Jaap M.; Haverkamp, Richard G.; Holroyd, Stephen E.; Jameson, Geoffrey B.; Mackenzie, D. D. S.; McCarthy, Owen J.

doi:10.1016/j.idairyj.2008.06.001

Cited by 100 publications

(86 citation statements)

References 39 publications

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“…Such heterogeneities in the MFGM have been re cently reported by Evers et al (2008) using different fluorescent dyes.…”

Section: Heterogeneities In the Lateral Organisation Of Polar Lipids mentioning

confidence: 60%

“…Also, WGA is able to bind to acidic glycosphingolipids (called gangliosides) which contain sialic acid. Using WGA, Evers et al (2008) previously reported chemical and then structural het erogeneities in the MFGM.…”

Section: Simultaneous Localisation Of Polar Lipids Glycolipids and Gmentioning

confidence: 99%

“…Using confocal laser scanning microscopy, Lopez, BriardBion, Beaucher, and Ollivon (2008) developed a protocol allowing the labelling of milk phospholipids in milk and dairy products such as cheese. More recently, Evers et al (2008) used lipophilic probes and fluorescent lectin wheat germ agglutinin (WGA) to investigate the organisation of the MFGM in milks from various species.…”

Section: Introductionmentioning

confidence: 99%

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Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins

Lopez¹,

Madec²,

2010

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This study reveals the lateral organisation ofthe milk fat globule membrane (MFGM). Using confocal laser scanning microscopy (CLSM) and a lipid soluble molecule, an exogenous phospholipid and two lectins as fluorescent probes we located triacylglycerols in the core of fat globules and investigated the organisation of the polar lipids and glycoproteins of the MFGM, in situ in milk, Lipid rafts corresponding to the lateral segregation of sphingolipids in liquid-ordered phases surrounded by liquid-disordered domains composed by the glycerophospholipids were observed in the MFGM. These lipid rafts which correspond to rigid sphingolipid-rich domains have a circular shape at room temperature. CLSM experiments revealed that glycoproteins and glycolipids are heterogeneously distributed around fat globules and that they are not located in the lipid rafts. The characterisations performed by in depth thin sectioning of fat globules and in dynamic as a function of time revealed chemical and structural heterogeneities in the MFGM. Schematic 3D and 20 representations of the MFGM are proposed and discussed. The physiological and nutritional consequences of the lateral organisation of polar lipids and glycoproteins in the MFGM are discussed but remain to be elucidated.

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“…Such heterogeneities in the MFGM have been re cently reported by Evers et al (2008) using different fluorescent dyes.…”

Section: Heterogeneities In the Lateral Organisation Of Polar Lipids mentioning

confidence: 60%

Section: Simultaneous Localisation Of Polar Lipids Glycolipids and Gmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins

Lopez¹,

Madec²,

2010

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“…Camel's milk phospholipids, such as phosphatidyl-ethanolamine (PE), phosphatidyl-choline (PC), lysophosphatidylcholine (LPC) and phosphatidylinositol (PI); proteins, including immunoglobulin are among well studied molecules in this regards [102]. Camel milk has unique immunoglobulin called the VHH (variable heavy heavy) antibodies or nanobodies devoid of the typical light chains present in normal antibodies.…”

Section: Camel's Milk and Urine Nanoparticlesmentioning

confidence: 99%

Therapeutic Applications of Camel’s Milk and Urine against Cancer: Current Development Efforts and Future Perspectives

Alebie¹,

Yohannes²,

Worku³

2017

J Cancer Sci Ther

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“…Confocal laser scanning microscopy, lipophilic probes, and fluorescent lectin wheat germ agglutinin (WGA) were first utilized to investigate the organization of the MFGM in milks from animal species (Evers et al, 2008). With the same confocal technique, Lopez et al (2010b) revealed the heterogeneous organization of the MFGM, especially the lateral structure of polar lipids, glycolipids, and glycoproteins.…”

Section: J Methodology To Monitor the Biological Activity Of The Mfgmmentioning

confidence: 99%

Effects of Milk Processing on the Milk Fat Globule Membrane Constituents

Elias-Argote¹

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Heterogeneity of milk fat globule membrane structure and composition as observed using fluorescence microscopy techniques

Cited by 100 publications

References 39 publications

Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins

Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins

Therapeutic Applications of Camel’s Milk and Urine against Cancer: Current Development Efforts and Future Perspectives

Effects of Milk Processing on the Milk Fat Globule Membrane Constituents

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