Heterogeneity of nuclear DNA pattern and its relationship with cell cycle activity parameters in multinodular goitre

Francia, Giuseppe; Azzolina, L.S.; Mantovani, T.; Davì, Maria Vittoria; Brazzarola, Paolo; Valerio, Adriano; Petronio, R; Sussi, P. L.

doi:10.1046/j.1365-2265.1997.1480961.x

Cited by 9 publications

(9 citation statements)

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“…We have also observed aneuploidy in 18.5% of cases of nodular goiter, a percentage which is in accordance with the previously reported range of 10-22% [13,18,30,37]. Most of the aneuploid nodules in the present series displayed either papillary hyperplasia or foci of fetal-embryonal growth pattern (data not shown) and may represent, according to some authors, true neoplasms rather than hyperplastic lesions [1].…”

Section: Discussionsupporting

confidence: 93%

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Fetal adenomas and minimally invasive follicular carcinomas of the thyroid frequently display a triploid or near triploid DNA pattern

et al. 2001

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The ploidy pattern and the percentage of S-phase cells were investigated by means of flow cytometry using fresh or frozen samples in a series of 143 tumors and tumor-like lesions of the thyroid in an attempt to find whether there is any relationship between the histological characteristics of the lesions and their DNA content. The percentages of aneuploidy cases per category were: nodular goiter, 18.5% (15/81); fetal adenoma (including cases with trabecular/solid growth pattern), 58.3% (14/24); follicular adenoma other than fetal adenoma, 0% (0/18); papillary carcinoma, 11.1% (1/9); and minimally invasive follicular carcinoma, 57.1% (4/7). Regardless of the histological category, aneuploid lesions had a significantly higher (P < 0.001) percentage of S-phase cells (7.3%) than diploid lesions (4.1%). All the six cases with a DNA content within the triploid range were fetal adenomas, but one was a follicular carcinoma displaying a fetal adenoma-like growth pattern. The other three follicular carcinomas with an aneuploid DNA pattern also displayed foci of fetal adenoma-like growth pattern. Image cytometry of the four aneuploid follicular carcinomas showed similar DNA indexes in the peripheral, invasive foci of the lesions and in the central fetal adenoma-like areas. These results demonstrate that aneuploidy in benign tumors is restricted to adenomas displaying a fetal or fetal/embryonal growth pattern and support the concept that chromosome instability is a major pathway of tumorigenesis in thyroid follicular neoplasms.

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Section: Discussionsupporting

confidence: 93%

“…In the benign lesions of the thyroid, the percentage of cases with abnormal DNA content varies from 10% to 22% in goiters [13,18,31,37] and from 18% to 52% in follicular adenomas [8,18,22,34]. The prevalence of abnormal DNA content is particularly high in Hürthle cell adenomas [6,7,40].…”

Section: Introductionmentioning

confidence: 99%

Fetal adenomas and minimally invasive follicular carcinomas of the thyroid frequently display a triploid or near triploid DNA pattern

et al. 2001

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“…For many years aneuploidy was considered as a sign of malignancy, but several studies have demonstrated that chromosomal instability (CIN) can also occur in benign diseases such as angioimmunoblastic lymphadenopathy, monoclonal gamapathy 2 and ulcerative colitis 3 . In thyroid lesions, aneuploidy is rare in PTCs and is observed in about 57% of FTCs, in 10–25% of FTAs 4 and in 10–22% of multinodular hyperplasias 5–7 …”

Section: Introductionmentioning

confidence: 99%

Aneuploidy and RAS mutations are mutually exclusive events in the development of well‐differentiated thyroid follicular tumours

Banito¹,

Pinto²,

Espadinha³

et al. 2007

Clinical Endocrinology

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“…Francis et al [45] explained the aneuploid nodular goitre cases by the possible development of chromosomal aberrations and/or the selection of cellular clones with high growth potential. Konarska et al [46] recorded the presence of nodular goitre cases with hypoploid DNA pattern; however they did not suggest an explanation.…”

Section: Galectin-3 X 400) 2-b: Follicular Carcinoma Showing Diffusementioning

confidence: 99%

Preoperative Evaluation of Thyroid Epithelial Lesions by DNA Ploidy and Galectin-3 Expression in FNAC

El-Sharkawy

AbdEl-Aal

Esmal

et al. 2014

Open Access Maced J Med Sci

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AIM:This study aimed to investigate the value of DNA ploidy and galectins-3 immunostain in the preoperative evaluation of thyroid epithelial lesions. MATERIAL AND METHODS:Sixty patients presenting with thyroid enlargement were included in this study and referred by clinicians for FNA. Routine cytological evaluation was done on PAP stained slides according to the WHO criteria and at least three slides were prepared for routine cytological examinations. The nuclear DNA analysis was performed at the Pathology Department, National Research Center using the Leica Qwin 500 Image Analyzer (LEICA Imaging Systems Ltd, Cambridge, England). Galectin-3 expression was investigated in all tissues using streptavidin-biotin technique. RESULTS:Conventional Fine needle aspiration cytology (FNAC) of 60 cases could diagnose malignancy with a sensitivity of 60%, negative predictive value (NPV) 71.4%, and overall diagnostic accuracy of 80%. The aneuploidy was significantly associated with malignancy, with sensitivity 90.9%, specificity 83.3% and accuracy 88.3%. On using galectin-3 immunocytochemichal stain on cell blocks prepared from FNA the values were improved, sensitivity 93.3% specificity 86.7% and overall accuracy 90% and it was noticed that galectin-3 over expression was significantly associated with malignancy. CONCLUSIONS:From the results of this study we can consider that DNA ploidy and Galectin-3 could refine the FNA results and increase its sensitivity as a screening test from sensitivity(60%) to reach sensitivity (93.3%), thus decreasing the false negative cases. From this study, it is concluded that the application of ancillary techniques as galectin-3 immunocytochemical markers may become a reliable indicator for surgical intervention, DNA ploidy measurements on the other hand may be of value in galectin-3 negative cases to determine the behavior of the lesion in such cases & refine the preoperative assessment by out ruling false negative cases.

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Heterogeneity of nuclear DNA pattern and its relationship with cell cycle activity parameters in multinodular goitre

Cited by 9 publications

References 0 publications

Fetal adenomas and minimally invasive follicular carcinomas of the thyroid frequently display a triploid or near triploid DNA pattern

Fetal adenomas and minimally invasive follicular carcinomas of the thyroid frequently display a triploid or near triploid DNA pattern

Aneuploidy and RAS mutations are mutually exclusive events in the development of well‐differentiated thyroid follicular tumours

Preoperative Evaluation of Thyroid Epithelial Lesions by DNA Ploidy and Galectin-3 Expression in FNAC

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