Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain

Kameyama, Takeshi; Miyata, Muneaki; Shiotani, Hajime; Adachi, Jun; Kakuta, Soichiro; Uchiyama, Yasuo; Mizutani, Kiyohito; Takai, Yoshimi

doi:10.1016/j.isci.2023.108010

Cited by 4 publications

(2 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent investigations suggest that most gray matter astrocytes link with at least one blood vessel (Hösli et al, 2022). However, emerging studies have also highlighted the heterogeneity in astrocyte end-feet architecture, suggesting diverse modes of interaction with blood vessels (Kameyama et al, 2023;Kubotera et al, 2019). Intriguingly, following the disruption of end feet or the stalk of astrocytic processes, some astrocytes exhibit the capacity to regenerate these structures while others do not (Kubotera et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Intriguingly, following the disruption of end feet or the stalk of astrocytic processes, some astrocytes exhibit the capacity to regenerate these structures while others do not (Kubotera et al, 2019). Furthermore, detailed analysis using mass spectrometry has unveiled a spectrum of sizes and molecular components within astrocyte end feet, indicating potential functional diversity in their interaction with blood vessels (Kameyama et al, 2023). This underscores a crucial distinction between astrocytes capable of developing end feet for direct contact with vessels and those whose somas maintain tight associations with blood vessels (Kubotera et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Spatial organization of astrocyte clones: The role of developmental progenitor timing

Ojalvo‐Sanz,

Pernia‐Solanilla,

López‐Mascaraque

2024

Glia

View full text Add to dashboard Cite

Astrocytes represent a diverse and morphologically complex group of glial cells critical for shaping and maintaining nervous system homeostasis, as well as responding to injuries. Understanding the origins of astroglial heterogeneity, originated from a limited number of progenitors, has been the focus of many studies. Most of these investigations have centered on protoplasmic and pial astrocytes, while the clonal relationship of fibrous astrocytes or juxtavascular astrocytes has remained relatively unexplored. In this study, we sought to elucidate the morphological diversity and clonal distribution of astrocytes across adult cortical layers, with particular emphasis on their ontogenetic origins. Using the StarTrack lineage tracing tool, we explored the characteristics of adult astroglial clones derived from single and specific progenitors at various embryonic stages. Our results revealed a heterogeneous spatial distribution of astroglial clones, characterized by variations in location, clonal size, and rostro–caudal dispersion. While a considerable proportion of clones were confined within specific cortical layers, others displayed sibling cells crossing layer boundaries. Notably, we observed a correlation between clone location and developmental stage at earlier embryonic stages, although this relationship diminished in later stages. Fibrous astrocyte clones were exclusively confined to the corpus callosum. In contrast, protoplasmic or juxtavascular clones were located in either the upper or lower cortical layers, with certain clones displayed sibling cells distributed across both regions. Our findings underscore the developmental origins and spatial distribution of astroglial clones within cortical layers, providing new insights into the interplay between their morphology, clonal sizes, and progenitor heterogeneity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Spatial organization of astrocyte clones: The role of developmental progenitor timing

Ojalvo‐Sanz,

Pernia‐Solanilla,

López‐Mascaraque

2024

Glia

View full text Add to dashboard Cite

show abstract

Crosstalk Among Glial Cells in the Blood–Brain Barrier Injury After Ischemic Stroke

Lu,

Wen

2024

Mol Neurobiol

View full text Add to dashboard Cite

RiboTag RNA Sequencing Identifies Local Translation of HSP70 In Astrocyte Endfeet After Cerebral Ischemia

Shim,

Ciryam,

Tosun

et al. 2024

Preprint

View full text Add to dashboard Cite

Brain ischemia causes disruption in cerebral blood flow and blood-brain barrier (BBB) integrity which are normally maintained by the astrocyte endfeet. Emerging evidence points to dysregulation of the astrocyte translatome during ischemia, but its effects on the endfoot translatome are unknown. In this study, we aimed to investigate the early effects of ischemia on the astrocyte endfoot translatome in a rodent model of cerebral ischemia-reperfusion. To do so, we immunoprecipitated astrocyte-specific tagged ribosomes (RiboTag IP) from mechanically isolated brain microvessels. In mice subjected to middle cerebral artery occlusion and reperfusion and contralateral controls, we sequenced ribosome-bound RNAs from perivascular astrocyte endfeet and identified 205 genes that were differentially expressed in the translatome after ischemia. Pathways associated with the differential expressions included proteostasis, inflammation, cell cycle, and metabolism. Transcription factors whose targets were enriched amongst upregulated translating genes included HSF1, the master regulator of the heat shock response. The most highly upregulated genes in the translatome were HSF1-dependent Hspa1a and Hspa1b, which encode the inducible HSP70. We found that HSP70 is upregulated in astrocyte endfeet after ischemia, coinciding with an increase in ubiquitination across the proteome. These findings suggest a robust proteostasis response to proteotoxic stress in the endfoot translatome after ischemia. Modulating proteostasis in endfeet may be a strategy to preserve endfeet function and BBB integrity after ischemic stroke.

show abstract

Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain

Cited by 4 publications

References 73 publications

Spatial organization of astrocyte clones: The role of developmental progenitor timing

Spatial organization of astrocyte clones: The role of developmental progenitor timing

Crosstalk Among Glial Cells in the Blood–Brain Barrier Injury After Ischemic Stroke

RiboTag RNA Sequencing Identifies Local Translation of HSP70 In Astrocyte Endfeet After Cerebral Ischemia

Contact Info

Product

Resources

About