Heterogeneity of Plasma High Density Lipoproteins*

Levy, Robert I.; Fredrickson, Donald S.

doi:10.1172/jci105156

Cited by 184 publications

(63 citation statements)

References 31 publications

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“…The levels of HDL2-ch and HDL2-pl also agreed with Levy and Fredrickson (1965) who reported that these levels occupy 25 to 32% of cholesterol and phospholipids contained in the HDL fraction.…”

Section: Hdl-pl Obtained By Precipitation and Hdl2+3-p1supporting

confidence: 88%

“…Because albumin is bound to phospholipids in HDL-pl determined by precipitation methods, the HDL-pl levels may not demonstrate the real phospholipids level in the HDL fraction. Likewise, albumin is usually contained in the HDL fraction determined by ultracentrifugal techniques (Levy and Fredrickson 1965;Patsch et al 1980). No investigator has evaluated HDL fraction phospholipids after the removal of albumin.…”

mentioning

confidence: 99%

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An evaluation of serum high density lipoproteins-phospholipids.

Ide

Tsuji

Shimada

et al. 1988

Tohoku J. Exp. Med.

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Phospholipids in high density lipoproteins (HDL) is being used as a negative risk indicator of atherosclerosis. Phospholipids in HDL may not demonstrate the actual level of HDLphospholipids when determined by the precipitation or ultracentrifugal methods, because HDL fractions contain very high density lipoproteins (VHDL) and albumin. In the present study, the true level of phospholipids in HDL was estimated using high performance liquid chromatography (HPLC), and it was compared with the level of phospholipids in HDL determined by the precipitation method. Sera from 18 healthy subjects were used as materials. In the HPLC method, the HDL fraction was extracted making sure that it contained no free albumin, which is albumin not bound to phospholipids. The HDL fraction was separated into subfractions. It was found that phospholipids in the VHDL fraction make a 20.2 + 7.3% (mean± S.D.) part of the total HDL-phospholipids. A large part of the VHDL fraction was constituted of albumin-bound phospholipids. A significant correlation was observed between HDL-phospholipids determined by the precipitation method, which contain albumin, and the actual HDL fraction phospholipids determined by HPLC, which do not contain VHDL (r = 0.903, p<0.01). These results suggest that HDL-phospholipids values determined by the precipitation method give useful clinical data.

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Section: Hdl-pl Obtained By Precipitation and Hdl2+3-p1supporting

confidence: 88%

mentioning

confidence: 99%

An evaluation of serum high density lipoproteins-phospholipids.

Ide

Tsuji

Shimada

et al. 1988

Tohoku J. Exp. Med.

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“…Lipoprotein fractions were isolated in the preparative ultracentrifuge by techniques identical to those previously described (12 hours to permit isolation of any very low density lipoprotein (VLDL) (13). Isotonic NaCl solution was usually added to the top and bottom fractions to return them to plasma concentrations before they were further utilized.…”

Section: Methodsmentioning

confidence: 99%

The lipoproteins and lipid transport in abetalipoproteinemia.

Levy¹,

Fredrickson²,

Laster³

1966

J. Clin. Invest.

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“…Plates were developed at 4°C and were observed for 7 days. The immunoelectrophoretic techniques used have been previously described (31,32). Rabbit antisera to LDL or apoLDL were produced by two injections into the foot pads of white New Zealand rabbits of 1.25 ml of protein solution (6-8 mg of protein) mixed with 0.75 ml of complete Freund's adjuvant.…”

Section: Immunological Techniquesmentioning

confidence: 99%

Evidence for the identity of the major apoprotein in low density and very low density lipoproteins in normal subjects and patients with familial hyperlipoproteinemia

Gotto¹,

Brown²,

Levy³

et al. 1972

J. Clin. Invest.

108

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A B S T R A C T The major apoprotein (s) from human plasma low density lipoproteins was isolated and compared with a major protein fraction (fraction I) from very low density lipoproteins (VLDL). Fraction I had been previously found to comprise approximately 40% of the total protein of VLDL. Fraction I from VLDL and apoLDL from normal subjects were indistinguishable in amino acid compositions and circular dichroic spectra. They yielded indistinguishable displacement curves of LDL-'I by radioimmunoassay and formed immunoprecipitin lines of complete identity. Fraction I from VLDL of normal subjects was compared with the fraction isolated from patients with familial types II, III, IV, and V hyperlipoproteinemia. There were no detectable differences between any of these fractions in amino acid compositions, circular dichroic spectra, and immunochemical properties. It was, therefore, concluded that short of peptide mapping or determination of amino acid sequences, fraction I from VLDL of each subject with familial hyperlipoproteinemia appears to be identical with fraction I and apoLDL from normal individuals.A new convenient method of preparation of soluble apoLDL, modified from a procedure previously described from this laboratory, is presented.

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Heterogeneity of Plasma High Density Lipoproteins*

Cited by 184 publications

References 31 publications

An evaluation of serum high density lipoproteins-phospholipids.

An evaluation of serum high density lipoproteins-phospholipids.

The lipoproteins and lipid transport in abetalipoproteinemia.

Evidence for the identity of the major apoprotein in low density and very low density lipoproteins in normal subjects and patients with familial hyperlipoproteinemia

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