Heterogeneity of Protein Profiles of <i>Helicobacter pylori</i> Isolated from Individual Patients

Kitamoto, Noritoshi; Nakamoto, Hiromichi; Katai, Atsuo; Takahara, Nobuaki; Nakata, Hajime; Tamaki, H; Tanaka, Tomoyuki

doi:10.1046/j.1523-5378.1998.08703.x

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Yakoob et al (2000) showed that two different H. pylori strains coexist in an infected individual and may not be uniformly distributed among biopsy sites [33]. Heterogeneity of protein profile of H. pylori isolated from individual patients was shown by Kitamoto et al (1998) [34]. Heterogenic nature of H. pylori strains among infected couples has also been reported from Taiwan [35].…”

Section: Discussionmentioning

confidence: 99%

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Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

Patra

Chattopadhyay

et al. 2012

PLoS ONE

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Helicobacter pylori is one of the most diverse bacterial species that chronically infects more than 70% of Indian population. Interestingly, data showing microdiversity of the H. pylori strains within a particular gastric niche remained scarce. To understand the extent of genetic diversity among H. pylori strains within a given host, 30 patients with gastro-duodenal problems were subjected to endoscopy and from each patient 10 single colonies were isolated. Characterization of each of these 10 single colonies by DNA fingerprinting as well as genotyping of several important genetic markers viz. cagA, vacA, iceA, vapD, cag PAI empty site, IS605, RFLP and two other genetic segments within cag PAI revealed that all of the 30 patients were infected with more than one strain and sometimes strains with 5 to 6 types of genetic variants. Analyses of certain genetic loci showed the microdiversity among the colonies from single patient, which may be due to the recombination events during long-term carriage of the pathogen. These results suggest that most of the patients have acquired H. pylori due to repeated exposure to this pathogen with different genetic make-up, which may increase the possibility of super infections. Genetic exchanges between these unrelated H. pylori strains may support certain H. pylori variant to grow better in a given host than the parental strain and thereby increasing the possibility for the severity of the infection.

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Section: Discussionmentioning

confidence: 99%

“…PCR- amplified vacA segments were digested with Hae III (Bangalore Genei) for 3 hrs at 37°C in the appropriate buffer recommended by the supplier and the digested DNA were analyzed on ethidium bromide stained 2% agarose gel [34], [43], [44]. PCR amplification of the vacA gene fragment was done using primers vas1F and vasGR [45].…”

Section: Methodsmentioning

confidence: 99%

Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

Patra

Chattopadhyay

et al. 2012

PLoS ONE

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“…Multiple infections by different strains of Hel. pylori (Beji et al, 1989 ;Owen et al, 1993 ;Fujimoto et al, 1994 ;Akopyants et al, 1995 ;Taylor et al, 1995 ;, as well as localized mixed occurrences (Kitamoto et al, 1998 ;J. C. Atherton, unpublished) have been reported, but there is no evidence that these strains compete via exponential growth.…”

Section: Discussionmentioning

confidence: 99%

Absence of translationally selected synonymous codon usage bias in Helicobacter pylori

2000

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Synonymous codon usage in the complete genome of Helicobacter pylori was investigated. The moderate AMT-richness of the genome (GMC l 39 mol %) is reflected in the overall synonymous codon usage but the frequencies of some codons cannot be explained by simple mutational biases. A low level of heterogeneity among genes was observed, but this does not appear to be due to varying mutational bias or translational selection. Some of the heterogeneity was due to amino acid composition variation among the encoded proteins, and some may be attributable to recent acquisition of genes from other species. Since Hel. pylori codon usage is not dominated by biased mutation patterns, the absence of evidence for translationally mediated selection among synonymous codons is striking. This has implications with regard to the life history of this species, and in particular suggests that Hel. pylori strains are not subject to periods of competitive exponential growth. Despite the lack of selected codon usage, base composition immediately after the translation initiation site is skewed, consistent with selection against secondary structure formation in this region.

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VacA Genotyping Directly from Gastric Biopsy Specimens and Estimation of Mixed Helicobacter pylori Infections in Patients with Duodenal Ulcer and Gastritis

Hennig

Trzeciak²,

Reguła³

et al. 1999

Scandinavian Journal of Gastroenterology

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Heterogeneity of Protein Profiles of Helicobacter pylori Isolated from Individual Patients

Abstract: These findings suggest that more than one antigenically different strain of H. pylori may exist in same infected individuals.

Cited by 4 publications

References 25 publications

Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India

Absence of translationally selected synonymous codon usage bias in Helicobacter pylori

VacA Genotyping Directly from Gastric Biopsy Specimens and Estimation of Mixed Helicobacter pylori Infections in Patients with Duodenal Ulcer and Gastritis

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