Heterogeneity of the barrier properties of the colon in rat

Abstract

The incidence of colorectal cancer in different parts of the large intestine is not the same, as tumors more often appear in the distal part of the colon compared to the proximal one. The purpose of this study was to investigate heterogeneity of the barrier properties of the colon and clarify the effects of Prostaglandin E2 and interleukin-1beta on its different parts. An in-depth analysis of short circuit current, transepithelial electrical resistance and paracellular permeability for sodium fluorescein in Ussing chambers showed that the proximal part of the colon was less permeable compared to the distal one and the substances had different effects on the parameters of permeability in different parts of the colon. We suppose that heterogeneity of the barrier properties of the colon and various effects of their regulation by local molecular agents may determine different incidence of pathologies in the colon.

Selective Role of TNFα and IL10 in Regulation of Barrier Properties of the Colon in DMH-Induced Tumor and Healthy Rats

“…Previously, we conducted a segmental analysis of the barrier properties of the colon [ 63 ]. It was shown that the barrier properties were more pronounced in the proximal part of the colon compared to the distal part.…”

“…Based on the literature, most studies of the barrier properties of the intestinal epithelium under cytokines action were carried out in vitro on cell cultures, with stimulation mainly from the basolateral side of the epithelium [ 37 , 64 , 65 ]. We stimulated the colon in situ from the apical side of the epithelium according to the approach we successfully used in our previous study [ 63 ]. We supposed that the addition of the cytokines to the mucosal side ensured their prolonged action and prevented their rapid enzymatic degradation by tissue cells.…”