Heterogeneous distribution of histone methylation in mature human sperm

Spina, Florenza Antonella la; Romanato, Marina; Brugo-Olmedo, Santiago; Vincentiis, Sabrina De; Julianelli, Vanina Laura; Rivera, Rocío Melissa; Buffone, Mariano G.

doi:10.1007/s10815-013-0137-4

Cited by 44 publications

(26 citation statements)

References 14 publications

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“…The levels of methylation of H3K27me3, which is a mark for transcriptional repression, were significantly higher than those of acetylation of H3K27ac, a marker for transcriptional activation. These findings agree with previous experimental evidence showing that methylation of histones cause transcriptional inactivity in mammals including humans (La Spina et al., ; Santos & Dean, ). The findings demonstrate that acetylated and trimethylated H3K27 are differentially retained in spermatozoa from bulls with different fertility indexes measured in vivo.…”

Section: Discussionsupporting

confidence: 93%

“…Therefore, these distinct and spatiotemporal PTMs of histone in conjunction with chromatin remodellers bromodomain containing protein 4 (BRD4; Bryant et al., ), chromodomain helicase DNA binding protein 5 (CHD5; Zhuang et al., ), testis‐specific histone 2B (TH2B; Montellier et al., ; Shinagawa et al., ) as well as other effector modellers play important roles in sperm chromatin remodelling, eviction of histones and proper replacement of histones with protamines. Additionally, increased H3 methylation in mature human spermatozoa relates to poor sperm motility and functional quality (La Spina et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Acetylation and methylation of sperm histone 3 lysine 27 (H3K27ac and H3K27me3) are associated with bull fertility

et al. 2017

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Epigenetic modifications in histones are crucial for proper sperm physiology, egg activation and reproductive development of males. The objectives of this study were to determine the conservation and interactomes of histone three (H3) and ascertain the expression dynamics of acetylated and methylated H3 lysine 27 (H3K27ac and H3K27me3) in spermatozoa from Holstein bulls with different fertility. Methods in immunocytochemistry and flow cytometry were used to evaluate the expression dynamics of H3K27ac and H3K27me3 in spermatozoa from 10 bulls with different in vivo fertility. Computational biology methods including Clustal Omega and Cytoscape were performed to determine the evolutionary conservation and interactome of H3. The post-translational modifications (PTM) of H3 (H3K27ac and H3K27me3) had different spatiotemporal dynamics in the sperm head. Intensities of methylation were higher than those of acetylation and inversely correlated between the two fertility groups (p = .0032). The interacting proteins of H3 are involved in critical subcellular processes such as regulation of methylation, nucleosome assembly, regulation of DNA replication and chromatin assembly. These results are significant because they help advance fundamental science and biotechnology of mammalian reproduction.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Acetylation and methylation of sperm histone 3 lysine 27 (H3K27ac and H3K27me3) are associated with bull fertility

et al. 2017

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“…La Spina and colleagues evaluated the methylation of H3K4Me, H3K4Me3, H3K9Me2, H3K79Me2, H3K36Me3, and acetylation of H3K4Ac and H4K5Ac in normal and abnormal human sperm. They reported the presence of heterogeneous histone modifications, and the presence of H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3 marks in poorly functional human sperm [79]. Yuen and colleagues made a knockout mouse model for the histone variant H3.3, H3f3b, which is involved in various biological processes including development, transcriptional memory, and transcriptional reprogramming.…”

Section: Role Of Histone Modifications In Male Infertilitymentioning

confidence: 99%

The role of epigenetics in idiopathic male infertility

Güneş

Arslan

Hekim

et al. 2016

J Assist Reprod Genet

109

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Infertility is a complex disorder with multiple genetic and environmental causes. Although some specific mutations have been identified, other factors responsible for sperm defects remain largely unknown. Despite considerable efforts to identify the pathophysiology of the disease, we cannot explain the underlying mechanisms of approximately half of infertility cases. This study reviews current data on epigenetic regulation and idiopathic male infertility. Recent data have shown an association between epigenetic modifications and idiopathic infertility. In this regard, epigenetics has emerged as one of the promising research areas in understanding male infertility. Many studies have indicated that epigenetic modifications, including DNA methylation in imprinted and developmental genes, histone tail modifications and short non-coding RNAs in spermatozoa may have a role in idiopathic male infertility.

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“…For example, H3K27ac and H3K27me3, modifications associated with enhancers and Polycomb silencing, respectively, are inversely correlated between high and low fertility bulls (Kutchy et al 2018). Even though studies are lacking in bovine IVF, studies in humans suggest that the level of retention as well as the type of modifications present can affect fertility (La Spina et al 2014).…”

Section: The Oocytementioning

confidence: 99%

Consequences of assisted reproductive techniques on the embryonic epigenome in cattle

Rivera¹

2020

Reprod. Fertil. Dev.

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Procedures used in assisted reproduction have been under constant scrutiny since their inception with the goal of improving the number and quality of embryos produced. However, in vitro production of embryos is not without complications because many fertilised oocytes fail to become blastocysts, and even those that do often differ in the genetic output compared with their in vivo counterparts. Thus only a portion of those transferred complete normal fetal development. An unwanted consequence of bovine assisted reproductive technology (ART) is the induction of a syndrome characterised by fetal overgrowth and placental abnormalities, namely large offspring syndrome; a condition associated with inappropriate control of the epigenome. Epigenetics is the study of chromatin and its effects on genetic output. Establishment and maintenance of epigenetic marks during gametogenesis and embryogenesis is imperative for the maintenance of cell identity and function. ARTs are implemented during times of vast epigenetic reprogramming; as a result, many studies have identified ART-induced deviations in epigenetic regulation in mammalian gametes and embryos. This review describes the various layers of epigenetic regulation and discusses findings pertaining to the effects of ART on the epigenome of bovine gametes and the preimplantation embryo.

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Heterogeneous distribution of histone methylation in mature human sperm

Abstract: Defects in the process of spermatogenesis may alter the correct epigenetic programming in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility.

Cited by 44 publications

References 14 publications

Acetylation and methylation of sperm histone 3 lysine 27 (H3K27ac and H3K27me3) are associated with bull fertility

Acetylation and methylation of sperm histone 3 lysine 27 (H3K27ac and H3K27me3) are associated with bull fertility

The role of epigenetics in idiopathic male infertility

Consequences of assisted reproductive techniques on the embryonic epigenome in cattle

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