Heterogeneous effects of individual high-fat diet compositions on phenotype, metabolic outcome, and hepatic proteome signature in BL/6 male mice

Abstract

The multitude of obesogenic diets used in rodent studies can hardly be overviewed. Since standardization is missing and assuming that individual compositions provoke individual effects, the choice of quality, quantity and combination of diet ingredients seems to be crucial for the outcome and interpretation of obesity studies. Therefore, the present study was conducted to compare the individual effects of three commonly used obesogenic diets, mainly differing in sugar and fat content. Besides basic phenotypic and metabolic characterization, one main aspect was a comparative liver proteome analysis. As expected, the obtained results picture differentiated consequences mainly depending on fat source and/or fat- and sugar quantity. By confirming the general presumption that the choice of nutritional composition is a pivotal factor, the present findings demonstrate that a conscious selection is indispensable for obtaining reliable and sound results in obesity research. In conclusion, we strongly recommend a careful selection of the appropriate diet in advance of a new experiment, taking into account the specific research question.

Effects of different obesogenic diets on joint integrity, inflammation and intermediate monocyte levels in a rat groove model of osteoarthritis

“…Moreover, the composition of the diet seems to affect the development of OA, with certain studies suggesting that high dietary sucrose intake leads to more joint degradation ( Collins et al, 2018 ; Donovan et al, 2018 ; Rios et al, 2019 ). Thus, in addition to the HF diet typically used in the groove OA model, we sought to investigate the effects of a high-fat/high-sucrose (HFS) diet, with a sucrose component reflective of unhealthy dietary habits observed in humans ( Johnson and Wardle, 2014 ; de Moura et al, 2021 ; Janoschek et al, 2023 ). By comparing chow, HF and HFS diet, we aimed to evaluate the impact of these distinct dietary compositions on OA pathogenesis within the context of the groove OA model, which represents metabolic OA patients with slowly progressing joint degeneration.…”