Heterogeneous expression of plasma-membrane-localised OsOSCA1.4 complements osmotic sensing based on hyperosmolality and salt stress in Arabidopsis osca1 mutant

Zhai, Yuanjun; Wen, Zhaohong; Han, Yang; Zhuo, Wenqing; Wang, Fang; Chen, Xi; Liu, Jin; Gao, Ping; Zhao, Heping; Wang, Yingdian; Wang, Youjun; Han, Shengcheng

doi:10.1016/j.ceca.2020.102261

Cited by 34 publications

(30 citation statements)

References 30 publications

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“…When the local Ca 2+ rises above threshold, via a CICR (Calcium Induced Calcium Release)-related a self-propagating Ca 2+ wave, it springs up in the indentation zone, ingresses the podium, and spreads all over the trap. We have documented that the podium differentially expresses DmOSCA1.7 a homolog of the mechanosensitive Ca 2+ channel AtOSCA1 (Zhai et al, 2020, Yuan et al, 2014, Thor et al, 2020. In the working model (Fig.…”

Section: Discussionmentioning

confidence: 93%

Ether Anesthetics Obstructs Touch-Induced Trigger Hair Calcium-Electrical Signals Preventing Excitation of The Venus Flytrap

Scherzer

Huang

Iosip

et al. 2021

Preprint

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Plants do not have neurons. Instead they operate transmembrane ion channels and can be electrically excited by physical and chemical clues. The Venus flytrap with its distinctive hapto-electric signaling is a prime example. When an insect collides with the trigger hairs emerging from the inner surface of the trap, the mechanical stimulus in the mechanosensory organ is translated into a calcium signal and an action potential (AP). Here we asked how a Ca 2+ wave and AP are initiated in the trigger hair and how these are fed into the systemic trap calcium-electric network. When the Dionaea muscipula trigger hair matures and develops hapto-electric excitability, the mechanosensitive anion channel DmMSL10 and voltage dependent SKOR type Shaker K + channel are expressed in the shear stress-sensitive podium, which interfaces with the flytrap’s prey capture and processing networks. In the excitable state, touch stimulation of the trigger hair first evokes a rise in the podium Ca 2+ , then the calcium signal together with an action potential, travel over the entire trap surface. Seeking the mechanisms that mediate touch-induced Ca 2+ transients in the mature trigger hairs, we show that OSCA1.7 and GLR3.6 type Ca 2+ channels and ACA2/10 Ca 2+ pumps are specifically expressed in the podium. In addition, we found that direct glutamate application to the trap evoked a propagating Ca 2+ and electrical event. Given that anesthetics affect K + channels and glutamate receptors in animal systems, we exposed flytraps to ether. An ether atmosphere suppressed the propagation of touch and glutamate-induced Ca 2+ and AP long-distance signaling, a response that was completely recovered when ether was replaced by fresh air. In line with ether targeting a calcium channel, so triggering a Ca 2+ activated anion channel, the AP amplitude declined before the electrical signal ceased completely. Ether in the mechanosensory organ neither prevented the touch induction of a calcium signal nor its post stimulus decay. This finding indicates that ether prevents the touch activated GLR3.6-expressing base of the trigger hair so exciting the capture organ.

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Section: Discussionmentioning

confidence: 93%

Ether Anesthetics Obstructs Touch-Induced Trigger Hair Calcium-Electrical Signals Preventing Excitation of The Venus Flytrap

Scherzer

Huang

Iosip

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Ether anesthetics prevents touch-induced trigger hair calcium-electrical signals excite the Venus flytrap

Scherzer

Huang

Iosip

et al. 2022

Sci Rep

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Plants do not have neurons but operate transmembrane ion channels and can get electrical excited by physical and chemical clues. Among them the Venus flytrap is characterized by its peculiar hapto-electric signaling. When insects collide with trigger hairs emerging the trap inner surface, the mechanical stimulus within the mechanosensory organ is translated into a calcium signal and an action potential (AP). Here we asked how the Ca2+ wave and AP is initiated in the trigger hair and how it is feed into systemic trap calcium-electrical networks. When Dionaea muscipula trigger hairs matures and develop hapto-electric excitability the mechanosensitive anion channel DmMSL10/FLYC1 and voltage dependent SKOR type Shaker K+ channel are expressed in the sheering stress sensitive podium. The podium of the trigger hair is interface to the flytrap’s prey capture and processing networks. In the excitable state touch stimulation of the trigger hair evokes a rise in the podium Ca2+ first and before the calcium signal together with an action potential travel all over the trap surface. In search for podium ion channels and pumps mediating touch induced Ca2+ transients, we, in mature trigger hairs firing fast Ca2+ signals and APs, found OSCA1.7 and GLR3.6 type Ca2+ channels and ACA2/10 Ca2+ pumps specifically expressed in the podium. Like trigger hair stimulation, glutamate application to the trap directly evoked a propagating Ca2+ and electrical event. Given that anesthetics affect K+ channels and glutamate receptors in the animal system we exposed flytraps to an ether atmosphere. As result propagation of touch and glutamate induced Ca2+ and AP long-distance signaling got suppressed, while the trap completely recovered excitability when ether was replaced by fresh air. In line with ether targeting a calcium channel addressing a Ca2+ activated anion channel the AP amplitude declined before the electrical signal ceased completely. Ether in the mechanosensory organ did neither prevent the touch induction of a calcium signal nor this post stimulus decay. This finding indicates that ether prevents the touch activated, glr3.6 expressing base of the trigger hair to excite the capture organ.

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“…Recent research found that Na + may bind to negatively charged components or to Na + binding sites such as GIPC to cause a conformational change and open the Ca 2+ channel ( Jiang et al, 2019 ). In addition, the mechanical force or ROS generated by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases in the plasma membrane may then activate components such as REDUCED HYPEROSMOLALITY-INDUCED [Ca 2+ ] i INCREASE1 (OSCA1) and cause Ca 2+ to flow into the cytoplasm ( Zhai et al, 2020 ).…”

Section: Sensing Of Salt Stressmentioning

confidence: 99%

“…The turgor pressure changes caused by osmotic stress regulate the opening of certain ion channels on the membrane. In Arabidopsis, OSCA1 is a high osmotic stress–gated Ca 2+ channel protein that is also considered as an osmotic stress sensor ( Yuan et al, 2014 ; Zhai et al, 2020 ). Ca 2+ -responsive phospholipid-binding BONZAI (BON) proteins play an important role in regulating all osmotic stress responses and are considered a kind of sensor in the membrane ( Chen et al, 2020 ; Saddhe et al, 2021 ).…”

Section: Known and Potential Salt-stress Sensors In Plant Cellsmentioning

confidence: 99%

Plant Salinity Sensors: Current Understanding and Future Directions

et al. 2022

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Salt stress is a major limiting factor for plant growth and crop yield. High salinity causes osmotic stress followed by ionic stress, both of which disturb plant growth and metabolism. Understanding how plants perceive salt stress will help efforts to improve salt tolerance and ameliorate the effect of salt stress on crop growth. Various sensors and receptors in plants recognize osmotic and ionic stresses and initiate signal transduction and adaptation responses. In the past decade, much progress has been made in identifying the sensors involved in salt stress. Here, we review current knowledge of osmotic sensors and Na+ sensors and their signal transduction pathways, focusing on plant roots under salt stress. Based on bioinformatic analyses, we also discuss possible structures and mechanisms of the candidate sensors. With the rapid decline of arable land, studies on salt-stress sensors and receptors in plants are critical for the future of sustainable agriculture in saline soils. These studies also broadly inform our overall understanding of stress signaling in plants.

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Heterogeneous expression of plasma-membrane-localised OsOSCA1.4 complements osmotic sensing based on hyperosmolality and salt stress in Arabidopsis osca1 mutant

Cited by 34 publications

References 30 publications

Ether Anesthetics Obstructs Touch-Induced Trigger Hair Calcium-Electrical Signals Preventing Excitation of The Venus Flytrap

Ether Anesthetics Obstructs Touch-Induced Trigger Hair Calcium-Electrical Signals Preventing Excitation of The Venus Flytrap

Ether anesthetics prevents touch-induced trigger hair calcium-electrical signals excite the Venus flytrap

Plant Salinity Sensors: Current Understanding and Future Directions

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