Heterogeneous inbred populations are useful as sources of near-isogenic lines for RAPD marker localization

Haley, Scott D.; Afanador, L.; Miklas, Phillip N.; Stavely, J. R.; Kelly, James D.

doi:10.1007/bf00223642

Cited by 65 publications

(51 citation statements)

References 23 publications

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“…The efficiency of NILs for targeting RAPD markers linked to disease resistance genes was suggested by Haley et al (1994c) as a strategy to reduce the number of false positive polymorphisms, and enhance the likelihood of finding tighter linkages to the gene of interest. In this study, primers that generated polymorphisms between NILs in the Middle American gene pool were tested on parents and bulks from the Andean gene pool.…”

Section: Discussionmentioning

confidence: 99%

“…The Middle American gene pool was represented by a F 4:7 recombinant inbred line (RIL) population derived from a cross between cultivars Bunsi with the I gene and Raven that carries the I, bc-3 gene combination (Kelly et al, 1994). The RILs were used to produce NILs that differed at the bc-3 locus, following a procedure outlined by Haley et al (1994c). The genotype of the NILs was verified by inoculation with the NL 3 strain of BCMNV.…”

Section: Mapping Populationsmentioning

confidence: 99%

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Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

et al. 2005

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SummaryNecrotic strains of bean common mosaic potyviruses are becoming increasingly problematic in bean growing areas of Africa and Europe. Pyramiding epistatic resistance genes provides the most effective long-term strategy for disease control against all known strains of the virus. Indirect selection using tightly linked markers should facilitate the breeding of desired epistatic resistance gene combinations. In common bean, the most effective strategy for broad spectrum control of the bean common mosaic potyviruses is to combine I and bc-3 genes. We describe the use of near-isogenic lines and segregating populations from different gene pools combined with bulked segregant analysis to identify markers tightly linked with the recessive bc-3 gene that conditions resistance to all strains of bean common mosaic necrosis virus. We identified a RAPD marker, OG6 595 , linked at 3.7 cM from the bc-3, and the marker was used to confirm the location of bc-3 gene on bean linkage group B6. A codominant AFLP marker, E ACA M CGG -169/172 was identified and linked at 3.5 cM from the bc-3 and the AFLP and OG6 595 markers flanked the bc-3 gene. The AFLP marker was converted to the STS marker SE ACA M CGG -134/137 which showed co-segregation with the original AFLP marker. The 134 bp fragment associated with resistance was linked with the bc-3 gene present in a diverse group of bean genotypes except in two kidney bean lines. The OG6 595 marker mapped on B6 supported independence of bc-3 from the I gene located on B2, which provides the opportunity to readily combine both genes in a single bean cultivar for broad spectrum resistance to bean common mosaic potyviruses.

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Section: Discussionmentioning

confidence: 99%

Section: Mapping Populationsmentioning

confidence: 99%

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

et al. 2005

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“…The Ur-4 gene was the first resistance gene tagged in bean , and represented the use of NILs and DNA bulking procedures prior to the publication of the BSA protocol of Michelmore et al (1991). In addition to the Ur-4 gene, six other rust resistance genes, Ur-3, Ur-5, Ur-7, Ur-9, Ur-11, and Ur-12 and three unnamed genes, one in BAC6 (Jung et al, 1996a,b) and two in Dorado (Miklas et al, 2000a), have been tagged (Haley et al, 1993(Haley et al, , 1994cJohnson et al, 1995;Jung et al, 1998;Park et al, 1999a,b). The utility of the markers for indirect selection of the Ur-3 and Ur-11 genes (Boone et al, 1999;Haley et al, 1994c;Johnson et al, 1995) has not been validated so their use has been limited.…”

Section: Bean Rustmentioning

confidence: 99%

Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea

Kelly

Gepts

Miklas

et al. 2003

Field Crops Research

256

240

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Gepts, P.; Miklas, P. N.; and Coyne, D. P., "Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea" (2003 The bean map is approximately 1200 cM with some 500 markers and an additional 500 markers shared with other bean maps. The cowpea map spans 2670 cM with over 400 markers. In addition to molecular markers, both maps include map locations of defense genes and phenotypic traits for disease and insect resistance, seed size, color and storage proteins, pod color and those traits associated with the domestication syndrome in bean. Since the bean and cowpea maps were developed independently, LGs with the same number probably refer to non-syntenic groups. Map locations of major resistance genes in bean are revealing gene clusters on LGs B1, B4, B7, and B11 for resistance to bean rust, anthracnose, common bacterial blight and white mold. Gene tagging and marker-assisted selection for disease resistance has progressed to a point where the indirect selection for resistance to a number of major diseases is now routine in bean breeding programs both in the US and overseas. #

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“…However, the development of NILs through repeated backcrossing is time-consuming and laborious (Tuinstra et al 1997). The use of a residual heterozygous line (RHL), as proposed by Yamanaka et al (2004), and which is derived from RIL, is a powerful tool for precisely evaluating QTL (Haley et al 1994). An RHL harbors a heterozygous region where the target QTL is located and a homozygous background in most other regions of the genome.…”

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confidence: 99%

Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3

et al. 2009

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Photosensitivity plays an essential role in the response of plants to their changing environments throughout their life cycle. In soybean [Glycine max (L.) Merrill], several associations between photosensitivity and maturity loci are known, but only limited information at the molecular level is available. The FT3 locus is one of the quantitative trait loci (QTL) for flowering time that corresponds to the maturity locus E3. To identify the gene responsible for this QTL, a map-based cloning strategy was undertaken. One phytochrome A gene (GmPhyA3) was considered a strong candidate for the FT3 locus. Allelism tests and gene sequence comparisons showed that alleles of Misuzudaizu (FT3/FT3; JP28856) and Harosoy (E3/E3; PI548573) were identical. The GmPhyA3 alleles of Moshidou Gong 503 (ft3/ft3; JP27603) and L62-667 (e3/e3; PI547716) showed weak or complete loss of function, respectively. High red/far-red (R/FR) long-day conditions enhanced the effects of the E3/FT3 alleles in various genetic backgrounds. Moreover, a mutant line harboring the nonfunctional GmPhyA3 flowered earlier than the original Bay (E3/E3; PI553043) under similar conditions. These results suggest that the variation in phytochrome A may contribute to the complex systems of soybean flowering response and geographic adaptation.

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Heterogeneous inbred populations are useful as sources of near-isogenic lines for RAPD marker localization

Cited by 65 publications

References 23 publications

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea

Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3

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