Heterogenous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies

Edwards, Paul A.W.

doi:10.1038/bjc.1985.24

Cited by 110 publications

(56 citation statements)

References 60 publications

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“…However, antigenic heterogenity was not restricted to tumour cells but was also seen in overlying, apparently normal, stratified squamous epithelium where there was a well defined gradation between positively staining superficial and negatively staining basal cells. This observation confirmed that heterogeneous TA-4 expression was not simply artifactual, but is in keeping with the current teaching that only a proportion of cells in either normal or malignant tissue will at any one time express a cytoplasmic antigen following application of the appropriate monoclonal antibody (Edwards, 1985). Indeed, heterogeneity of TA-4 expression in many cases made it difficult to score a given tumour as simply positive or negative.…”

Section: Discussionsupporting

confidence: 85%

Cellular localisation of tumour antigen (TA-4) in normal, dysplastic and neoplastic squamous epithelia of the upper aerodigestive tract

Stenzel²,

Sculley³

et al. 1990

Br J Cancer

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Summary We report the use of tumour antigen (TA-4) polyclonal antiserum to assess the level and pattern of TA-4 antigen expression in formalin-fixed paraffin-embedded tissue sections from 110 patients with a range of normal, dysplastic and malignant squamous epithelia from various sites in the upper aerodigestive tract. There was a high degree of TA-4 antigen expression in the superficial layers of normal squamous epithelium and in well-differentiated squamous cell cancers (SCC). TA-4 expression was consistently absent in dysplastic oral squamous epithelium and in poorly differentiated SCCs. The degree of cellular heterogeneity in moderately differentiated SCCs was such that morphologically identical squamous cancer cells could be distinguished on the basis of TA-4 expression. Immuno-electron microscopy localised Tumour-associated antigen (TA-4) is a glycoprotein of molecular weight 48 kDa originally extracted and subsequently purified by Kato and Torigoe (1977) from a patient with squamous cell cancer (SCC) of the uterine cervix. Subsequent studies using immuno-histochemistry and a radioimmunoassay technique have reported that the expression of TA-4 antigen in cervical squamous carcinoma is related to tumour cell differentiation (Maruo et al., 1985) and that serial serum levels of antigen are valuable in monitoring a patient's progress following completion of definitive treatment (Maruo et al., 1985;Kato et al., 1979).The only other study dealing with tissue localisation of TA-4 antigen reported the invariable presence of TA-4 in normal differentiated cervical squamous cells and in differentiated SCCs (Maruo et al., 1985). Our present study was undertaken in an attempt to further define the cellular localisation and cellular specificity of this antigen, and to determine whether previous results in relation to cervical cancer are also applicable to SCCs of the head and neck (oral cavity, larynx, pharynx) region. Materials and methodsTissue specimens Specimens were obtained from 110 patients with either leukoplakia or invasive squamous cell cancers, of various sites in the head and neck region (oral cavity 72, larynx 18, pharynx 10). These consisted of 85 males and 25 females, with a mean age of 62.5 years (range 28-82 years). Each specimen had been formalin-fixed and paraffin-embedded within 60 min of removal. Serial 4 ,im thick sections were cut from each block, and one slide was stained with haematoxylin and eosin for routine histopathological examination. Histological grading was performed by R.C. There was a range of patterns within each tumour. When there was predominantly keratinisation with squamous epithelial pearls, the tumour was graded as well differentiated. When keratinisation was present only in some areas of the tumour, it was graded as poorly differentiated. When there appeared to be a fairly even mixture of keratinising areas and non-keratinising areas, the tumour was graded as moderately well differentiated. Some keratinisation was present in all of the tumours studied. ImmunohistochemistryFo...

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Section: Discussionsupporting

confidence: 85%

Cellular localisation of tumour antigen (TA-4) in normal, dysplastic and neoplastic squamous epithelia of the upper aerodigestive tract

Stenzel²,

Sculley³

et al. 1990

Br J Cancer

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“…Although partial and complete responses have been reported with antibodies directed against lymphomas (Grossbard et al, 1992), reports of responses in colorectal cancer patients are limited (Begent el al., 1989). This has been attributed, in part, to the heterogeneity of antigen distribution (Edwards, 1985) and is illustrated by the wide variation between patients in amount of antibody (per cent injected activity per kg) localising in tumour. Attempts to investigate which parameters are responsible for this variation have implicated a number of factors (Shockley et al, 1991).…”

mentioning

confidence: 99%

Localisation of monoclonal antibodies reacting with different epitopes on carcinoembryonic antigen (CEA) – implications for targeted therapy

Boxer

Abassi²,

Pedley³

et al. 1994

Br J Cancer

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“…Variations in the expression of various compounds during the cell cycle, unstable genotypes and/or unstable phenotypes have been suggested (Arends et at., 1984b;Edwards 1985;Fidler, 1978;McCormack, 1984;Nowell, 1976;Olsson et at., 1984). The observation of more or less large foci of antigenically defilned cancer cells suggests that, at least in some cases, coexistence of several clones might be one of the correct explanations.…”

Section: Resultsmentioning

confidence: 99%

Antigenic heterogeneity and individuality in adenocarcinomas of the rectum and their secondaries

Enblad¹,

Glimelius²,

Busch³

et al. 1987

Br J Cancer

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Heterogenous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies

Cited by 110 publications

References 60 publications

Cellular localisation of tumour antigen (TA-4) in normal, dysplastic and neoplastic squamous epithelia of the upper aerodigestive tract

Cellular localisation of tumour antigen (TA-4) in normal, dysplastic and neoplastic squamous epithelia of the upper aerodigestive tract

Localisation of monoclonal antibodies reacting with different epitopes on carcinoembryonic antigen (CEA) – implications for targeted therapy

Antigenic heterogeneity and individuality in adenocarcinomas of the rectum and their secondaries

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