Heteroleptic pincer palladium(II) complex coated orthopedic implants impede the AbaI/AbaR quorum sensing system and biofilm development by <i>Acinetobacter baumannii</i>

Sowndarya, Jothipandiyan; Suresh, D.; Sankaran, Sankaran Venkatachalam; Thamotharan, Subbiah; Kumaravel, S; Vincent, Preethi; Sekaran, Saravanan; Gowrishankar, Shanmugaraj; Pandian, Shunmugiah Karutha; Nithyanand, Paramasivam

doi:10.1080/08927014.2021.2015336

Cited by 11 publications

(12 citation statements)

References 57 publications

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“…These drugs may target the AbaI receptor with a subsequent decrease in the autoinducers and a decrease in the expression of the regulator gene rather than direct modulation of AbaR activity and, therefore, quenching the QS cascade. Likewise, the molecular docking analysis of nervonic and oleic acid (Khadke et al, 2021) and Pd(II)‐E (Jothipandiyan et al, 2022) proved the potent binding affinity with AbaI accompanied QSI activity.…”

Section: Discussionmentioning

confidence: 99%

Ketoprofen, piroxicam and indomethacin-suppressed quorum sensing and virulence factors in Acinetobacter baumannii

El-Shaer

Shaldam

Shaaban

2022

Journal of Applied Microbiology

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Aim Quorum sensing (QS) inhibition is a promising strategy to suppress bacterial virulence and control infection caused by Gram‐negative and Gram‐positive bacteria. This study explores the QS inhibiting activity of the non‐steroidal anti‐inflammatory drugs (NSAIDs) in Acinetobacter baumannii. Methods and Results Ketoprofen, piroxicam and indomethacin revealed QS inhibition via elimination of violacein production of the reporter strain Chromobacterium violaceum ATCC 12472 without affecting bacterial growth. The minimal inhibitory concentration (MIC) of ketoprofen, piroxicam and indomethacin was determined against A. baumannii strains ATCC 17978, ATCC 19606, A1, A11 and A27 by the microbroth dilution method. The MICs of ketoprofen against tested isolates were 0.7–6.25 mg ml−1, piroxicam MICs were 1.25–2.5 mg ml−1, and indomethacin MICs were 3.12–12.5 mg ml−1. Those compounds significantly inhibited QS‐associated virulence factors such as biofilm formation, and surface motility, as well as, significantly increased bacterial tolerance to oxidative stress without affecting bacterial growth. On the molecular level, the three compounds significantly inhibited the transcription of QS regulatory genes abaI/abaR and biofilm‐regulated genes cusD and pgaB. Molecular docking analysis revealed the potent binding affinity of the three compounds with AbaI via hydrogen and/or hydrophobic bonds. Conclusion These results indicate that NSAIDs, ketoprofen, piroxicam and indomethacin, could be potential inhibitors of the QS and could suppress the QS‐related virulence factors of A. baumannii. Significance and Impact Ketoprofen, piroxicam and indomethacin could provide promising implications and strategies for combating the virulence and pathogenesis of A. baumannii.

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Section: Discussionmentioning

confidence: 99%

Ketoprofen, piroxicam and indomethacin-suppressed quorum sensing and virulence factors in Acinetobacter baumannii

El-Shaer

Shaldam

Shaaban

2022

Journal of Applied Microbiology

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“…The minimum inhibitory concentration is the lowest concentration that inhibits the growth of bacteria. In our previous study, Pd(II)- E exhibited a MIC of 300 µg/mL against monospecies of Methicillin-resistant Staphylococcus aureus and A. baumannii [ 26 , 27 ]. Therefore, the same MIC concentration was used in the present study for MRSA and A. baumannii mono-species.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, the biofilm inhibitory concentration of monospecies of MRSA and A. baumannii in microbial culture media (soybean casein digest) was found to be 150 µg/mL [ 26 , 27 ]. Therefore, the same concentration was used in artificial synovial fluid to check whether Pd(II)- E is effective in ASF.…”

Section: Methodsmentioning

confidence: 99%

“…Hence, Ti6Al-4V of size 1 × 1 cm was used throughout the study. Pd(II)- E at different concentrations (150 µg/mL for monospecies and 250 µg/mL for dual-species biofilms studies) were coated over the entire 1 × 1 cm titanium plates according to Sowndarya et al, 2021 [ 26 ]. Uncoated titanium plates served as controls.…”

Section: Methodsmentioning

confidence: 99%

“…To deal with antimicrobial resistance, we have synthesized novel thiazolinyl picolinamide complexes [ 25 ]. Our previous studies manifested that coating a Palladium(II) complex of N ∩ N ∩ N chelating thiazolinyl-picolinamide on a titanium implant effectively thwarted the virulence and biofilms of vital orthopedic implant infection-causing pathogens MRSA and A. baumannii [ 26 , 27 ]. Inspired by the above results, we were curious to know if the complexes could eradicate polymicrobial biofilms.…”

Section: Introductionmentioning

confidence: 99%

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Palladium(II) Metal Complex Fabricated Titanium Implant Mitigates Dual-Species Biofilms in Artificial Synovial Fluid

et al. 2023

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Metallodrugs have a potent application in various medical fields. In the current study, we used a novel Palladium(II) thiazolinyl picolinamide complex that was directly fabricated over the titanium implant to examine its potency in inhibiting dual-species biofilms and exopolysaccharides. Additionally, inhibition of mono- and dual-species biofilms by coated titanium plates in an in vitro joint microcosm was performed. The study was carried out for 7 days by cultivating mono- and dual-species biofilms on titanium plates placed in both growth media and artificial synovial fluid (ASF). By qPCR analysis, the interaction of co-cultured biofilms in ASF and the alteration in gene expression of co-cultured biofilms were studied. Remarkable alleviation of biofilm accumulation and EPS secretion was observed on the coated titanium plates. The effective impairment of biofilms and EPS matrix of biofilms on Pd(II)-E-coated titanium plates were visualized by Scanning Electron Microscopy. Moreover, coated titanium plates improved the adhesion of osteoblast cells, which is crucial for a bone biomaterial. The potential bioactivity of coated plates was also confirmed at the molecular level using qPCR analysis. The stability of coated plates in ASF for 7 days was examined with FESEM-EDAX analysis. Collectively, the present study provided an excellent anti-infective effect on Pd(II)-E-coated titanium plates without affecting their biocompatibility with bone cells.

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Motility of Acinetobacter baumannii: regulatory systems and controlling strategies

Jeong,

Khan,

Tabassum

et al. 2023

Appl Microbiol Biotechnol

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Heteroleptic pincer palladium(II) complex coated orthopedic implants impede the AbaI/AbaR quorum sensing system and biofilm development by Acinetobacter baumannii

Abstract: Table S3. Details of binding energy, inhibition constant and active site residues.

Cited by 11 publications

References 57 publications

Ketoprofen, piroxicam and indomethacin-suppressed quorum sensing and virulence factors in Acinetobacter baumannii

Ketoprofen, piroxicam and indomethacin-suppressed quorum sensing and virulence factors in Acinetobacter baumannii

Palladium(II) Metal Complex Fabricated Titanium Implant Mitigates Dual-Species Biofilms in Artificial Synovial Fluid

Motility of Acinetobacter baumannii: regulatory systems and controlling strategies

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