Heterologous cell contacts and metabolic coupling in bovine cumulus oocyte complexes

Loos, Friedemann; Kastrop, P.M.M.; Maurik, P. van; Beneden, Th.H. van; Kruip, Th.A.M.

doi:10.1002/mrd.1080280307

Cited by 82 publications

(46 citation statements)

References 16 publications

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“…These data support the idea that maintaining the cumulus-oocyte connection is essential for oocyte cytoplasmic maturation. Furthermore, the relative smaller oocyte diameter in the 0L-COC group (;99.7 lm) indicated that these oocytes might originate from less developed follicles [40,41], and the lack of surrounding cumulus suggested that they might be from earlier atretic follicles [42]. Compared to the ML-COC group, there were fewer oocytes in the 1L-COC group that progressed to MII.…”

Section: Discussionmentioning

confidence: 88%

In Vitro Oocyte Maturation and Preantral Follicle Culture from the Luteal-Phase Baboon Ovary Produce Mature Oocytes

Fazleabas

Shikanov

et al. 2010

Biology of Reproduction

107

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Female cancer patients who seek fertility preservation but cannot undergo ovarian stimulation and embryo preservation may consider 1) retrieval of immature oocytes followed by in vitro maturation (IVM) or 2) ovarian tissue cryopreservation followed by transplantation or in vitro follicle culture. Conventional IVM is carried out during the follicular phase of menstrual cycle. There is limited evidence demonstrating that immature oocyte retrieved during the luteal phase can mature in vitro and be fertilized to produce viable embryos. While in vitro follicle culture is successful in rodents, its application in nonhuman primates has made limited progress. The objective of this study was to investigate the competence of immature luteal-phase oocytes from baboon and to determine the effect of folliclestimulating hormone (FSH) on baboon preantral follicle culture and oocyte maturation in vitro. Oocytes from small antral follicle cumulus-oocyte complexes (COCs) with multiple cumulus layers (42%) were more likely to resume meiosis and progress to metaphase II (MII) than oocytes with a single layer of cumulus cells or less (23% vs. 3%, respectively). Twenty-four percent of mature oocytes were successfully fertilized by intracytoplasmic sperm injection, and 25% of these developed to morula-stage embryos. Preantral follicles were encapsulated in fibrin-alginate-matrigel matrices and cultured to small antral stage in an FSH-independent manner. FSH negatively impacted follicle health by disrupting the integrity of oocyte and cumulus cells contact. Follicles grown in the absence of FSH produced MII oocytes with normal spindle structure. In conclusion, baboon luteal-phase COCs and oocytes from cultured preantral follicles can be matured in vitro. Oocyte meiotic competence correlated positively with the number of cumulus cell layers.This study clarifies the parameters of the follicle culture system in nonhuman primates and provides foundational data for future clinical development as a fertility preservation option for women with cancer.

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Section: Discussionmentioning

confidence: 88%

In Vitro Oocyte Maturation and Preantral Follicle Culture from the Luteal-Phase Baboon Ovary Produce Mature Oocytes

Fazleabas

Shikanov

et al. 2010

Biology of Reproduction

107

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“…Intercellular communications within the ovarian follicle have been implicated in the control of meiotic arrest and maturation of mammalian oocytes, and such a metabolic coupling in cumulus-oocyte complexes (COCs) before the beginning of maturation has been demonstrated to be essential in the acquisition of developmental competence [10].…”

Section: Introductionmentioning

confidence: 99%

Role of Intracellular Cyclic Adenosine 3′,5′-Monophosphate Concentration and Oocyte-Cumulus Cells Communications on the Acquisition of the Developmental Competence During In Vitro Maturation of Bovine Oocyte1

Luciano

Modina

Vassena

et al. 2004

Biology of Reproduction

139

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The present study was designed to address the physiological role played by cAMP on gap junction (GJ) mediated communications between oocyte and cumulus cells during in vitro maturation. Cyclic AMP was stimulated by different collection and maturation media known to induce different rates of nuclear maturation and developmental competence as well as different levels of cumulus expansion. Cumulus-oocyte complexes (COCs) were matured for 0, 3, 7, 12, 18, and 24 h in the absence of stimulation or in the presence of serum and gonadotropins (fetal bovine serum+human menopausal gonadotropins [FCS+hMG]) or 0.01 microg/ml of invasive adenylate cyclase (iAC). For each time point, intracellular cAMP concentration ([cAMP]i) was determined either in the whole COC or oocyte after cumulus cell removal. GJ functional status was analyzed by microinjection of Lucifer yellow fluorescent dye in cumulus-enclosed oocytes and by immunohistochemical localization of connexin 43 (Cx43). In the absence of stimulation, [cAMP]i in COC and oocyte was lower than in other groups, and communications declined after 3 h of culture. In the FCS+hMG group, [cAMP]i increased significantly in COC, with a peak between 3 and 7 h that was temporally correlated with the beginning of the cumulus expansion process, which occurred only in this group and with the termination of the communications. COC matured in the presence of iAC showed a moderate increase of [cAMP]i during all of the maturation times as well as a prolongation of oocyte-cumulus cell communications. The immunohistochemical localization of Cx43 confirmed the delay in connexons protein turnover in iAC-treated COCs. Our results show that cumulus expansion and oocyte developmental competence are induced by different levels of cAMP and that its intracellular concentration may affect cell coupling between oocyte and cumulus cells. We hypothesize that the higher developmental competence of COCs matured in the presence of iAC could be achieved through a moderate increase of intracellular cAMP, which in turn determines a prolongation of communications between the two cell types.

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“…Este sucesso limitado pode ser parcialmente atribuído aos métodos de seleção dos COCs, baseados exclusivamente em parâmetros morfológicos avaliados ao estereomicroscópio, tais como a morfologia do ooplasma e do cumulus, o tamanho do folículo e do ovócito, e o grau de atresia folicular. Os COCs com massa de cumulus compacta e constituída por numerosas camadas de células foliculares, e com um ovócito de ooplasma homogêneo, apresentam maior capacidade de desenvolvimento, contrastando com os COCs de cumulus expandido e ooplasma heterogêneo (De Loos et al, 1989, 1991.…”

Section: Introductionunclassified

Estudo estereológico comparativo de complexos cumulus-ovócito aspirados de folículos durante o ciclo estral em bovinos

Calado

Rocha

Colaço

et al. 2005

Arq. Bras. Med. Vet. Zootec.

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Estudo estereológico comparativo de complexos cumulus-ovócito aspirados de folículos durante o ciclo estral em bovinos RESUMORealizou-se uma análise estereológica comparativa de complexos cumulus-ovócito (COCs) de bovino da raça Holtein-Friesian aspirados de folículos antrais pequenos (com diâmetro de 1-4mm) e médios (com diâmetro de 4-8mm) durante as fases de metaestro, diestro e de proestro. Foram estimados o volume médio dos COCs, dos ovócitos (com e sem zona pelúcida), dos núcleos dos ovócitos e das células foliculares e seus respectivos núcleos. Estimou-se a espessura da zona pelúcida e calculou-se a percentagem relativa da freqüência dos diferentes tipos de células foliculares encontradas no cumulus. Os folículos pequenos apresentaram crescimento acelerado e sem sincronia entre o volume do citoplasma e o do núcleo. No folículo médio ocorreu expansão harmoniosa núcleo-citoplasmática. Identificaram-se três populações de células foliculares (C1, C2 e C3), cuja distribuição na massa do cumulus é independente de sua posição relativamente ao ovócito. Durante o ciclo estral, as células C1 foram progressivamente substituídas por C2 e estas, por C3.Palavras-chave: bovino, célula folicular, ciclo estral, COC, estereologia ABSTRACT A comparative stereological analysis was performed in cumulus-oocyte complexes from HolsteinFriesian cows, aspirated from small (with diameter of 1-4mm) and medium (with diameter of 4-8mm) antral follicles during metestrous, diestrous and proestrous. The mean volumes of COCs, oocytes (with and without zona pellucida), nucleus of the oocyte, as well as the volumes of the follicular cells and their

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Heterologous cell contacts and metabolic coupling in bovine cumulus oocyte complexes

Cited by 82 publications

References 16 publications

In Vitro Oocyte Maturation and Preantral Follicle Culture from the Luteal-Phase Baboon Ovary Produce Mature Oocytes

In Vitro Oocyte Maturation and Preantral Follicle Culture from the Luteal-Phase Baboon Ovary Produce Mature Oocytes

Role of Intracellular Cyclic Adenosine 3′,5′-Monophosphate Concentration and Oocyte-Cumulus Cells Communications on the Acquisition of the Developmental Competence During In Vitro Maturation of Bovine Oocyte1

Estudo estereológico comparativo de complexos cumulus-ovócito aspirados de folículos durante o ciclo estral em bovinos

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