Heterologous expression and biochemical characterisation of cyanotoxin biosynthesis pathways

Cullen, Alescia; Pearson, Leanne A.; Mazmouz, Rabia; Liu, Tianzhe; Soeriyadi, Angela H.; Ongley, Sarah E.; Neilan, Brett A.

doi:10.1039/c8np00063h

Cited by 23 publications

(19 citation statements)

References 230 publications

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“…Chemical and spectral data show that the lyngbyatoxin molecule is comprised of an indolactam ring, with an attached linalyl side group at C-7 (Figure 2E) [83]. So far, seven natural lyngbyatoxin analogues have been identified, namely lyngbyatoxin A-C, 12- epi -lyngbyatoxin A, 2-oxo-3( R )-hydroxy-lyngbyatoxin A, 2-oxo-3( R )-hydroxy- 13-N-desmethyllyngbyatoxin A and 2,3-seco-2,3-dioxolyngbyatoxin A [32,34,35]. Lyngbyatoxins are highly inflammatory and vesicatory substances derived from marine cyanobacteria [84].…”

Section: Structural Characterizationmentioning

confidence: 99%

“…Lyngbyatoxins were first isolated from the lipid extract of a Hawaiian shallow-water variety of Lyngbya majuscula in 1979 [83]. Lyngbyatoxins have thus far been detected in the blooms of Lyngbya , Oscillatoria and Schizothrix [32,33]. A recent study found that it was also present in the Campania coast of southern Italy [87] (Table 1).…”

Section: Structural Characterizationmentioning

confidence: 99%

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The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

Liu

Yuan

et al. 2019

Toxins

137

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The widespread distribution of cyanobacteria in the aquatic environment is increasing the risk of water pollution caused by cyanotoxins, which poses a serious threat to human health. However, the structural characterization, distribution and identification techniques of cyanotoxins have not been comprehensively reviewed in previous studies. This paper aims to elaborate the existing information systematically on the diversity of cyanotoxins to identify valuable research avenues. According to the chemical structure, cyanotoxins are mainly classified into cyclic peptides, alkaloids, lipopeptides, nonprotein amino acids and lipoglycans. In terms of global distribution, the amount of cyanotoxins are unbalanced in different areas. The diversity of cyanotoxins is more obviously found in many developed countries than that in undeveloped countries. Moreover, the threat of cyanotoxins has promoted the development of identification and detection technology. Many emerging methods have been developed to detect cyanotoxins in the environment. This communication provides a comprehensive review of the diversity of cyanotoxins, and the detection and identification technology was discussed. This detailed information will be a valuable resource for identifying the various types of cyanotoxins which threaten the environment of different areas. The ability to accurately identify specific cyanotoxins is an obvious and essential aspect of cyanobacterial research.

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Section: Structural Characterizationmentioning

confidence: 99%

Section: Structural Characterizationmentioning

confidence: 99%

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

Liu

Yuan

et al. 2019

Toxins

137

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“…It was demonstrated that it had superior properties compared to previous reports of inducible promoter systems in cyanobacteria (Kelly et al, 2018). The native promoter from M. aeruginosa PCC 7806(native producer) was replaced for the heterologous production of microcystin-LR and [D-Asp3] microcystin-LR from E. coli GB05-MtaA (Cullen et al, 2018). Similarly, the recombinant microcystin synthetases gene cluster from M. aeruginosa PCC 7806 was heterologously expressed in E. coli to yield significant titer of [d-Asp3] microcystin-LR and microcystin-LR.…”

Section: Heterologous Production Of Cyanobacterial Compoundsmentioning

confidence: 99%

Recent Advances in Exploration and Biotechnological Production of Bioactive Compounds in Three Cyanobacterial Genera: Nostoc, Lyngbya, and Microcystis

Thuần

Shrestha

et al. 2019

Front. Chem.

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Cyanobacteria, are only Gram-negative bacteria with the capacity of oxygenic photosynthesis, so termed as “Cyanophyta” or “blue-green algae.” Their habitat is ubiquitous, which includes the diverse environments, such as soil, water, rock and other organisms (symbiosis, commensalism, or parasitism, etc.,). They are characterized as prominent producers of numerous types of important compounds with anti-microbial, anti-viral, anti-inflammatory and anti-tumor properties. Among the various cyanobacterial genera, members belonging to genera Nostoc, Lyngbya, and Microcystis possess greater attention. The major reason for that is the strains belonging to these genera produce the compounds with diverse activities/structures, including compounds in preclinical and/or clinical trials (cryptophycin and curacin), or the compounds retaining unique activities such as protease inhibitor (micropeptins and aeruginosins). Most of these compounds were tested for their efficacy and mechanism of action(MOA) through in vitro and/or in vivo studies. Recently, the advances in culture techniques of these cyanobacteria, and isolation, purification, and chromatographic analysis of their compounds have revealed insurmountable novel bioactive compounds from these cyanobacteria. This review provides comprehensive update on the origin, isolation and purification methods, chemical structures and biological activities of the major compounds from Nostoc, Lyngbya, and Microcystis. In addition, multi-omics approaches and biotechnological production of compounds from selected cyanobacterial genera have been discussed.

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“…Cyanotoxins are therefore an important group of chemical compounds, from the perspective of ecotoxicology, toxicology and environmental chemistry (Cullen et al, 2019). Cyanotoxins can be divided on the basis of two main criteria: their action mechanism in land vertebrates, especially mammals, in three principal classes (hepatotoxins, neurotoxins and dermatotoxins) (Woese, 1987) and their chemical structure, within which they may be classified as cyclic peptides, alkaloids or lipopolysaccharides (LPS) (Cohen & Gurevitz, 2006;Ferrão-Filho & Kozlowsky-Suzuki, 2011;Zanchett & Oliveira-Filho, 2013).…”

mentioning

confidence: 99%

Toxic compounds produced by cyanobacteria belonging to several species of the order Nostocales: A review

Nowruzi

Porzani

2020

J of Applied Toxicology

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Heterologous expression and biochemical characterisation of cyanotoxin biosynthesis pathways

Abstract: This review discusses cyanotoxin biosynthetic pathways and highlights the heterologous expression and biochemical studies used to characterise them.

Cited by 23 publications

References 230 publications

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

Recent Advances in Exploration and Biotechnological Production of Bioactive Compounds in Three Cyanobacterial Genera: Nostoc, Lyngbya, and Microcystis

Toxic compounds produced by cyanobacteria belonging to several species of the order Nostocales: A review

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