Heterologous expression and characterization of Anaeromyces robustus xylanase and its use in bread making

Liu, Zhenyang; Wen, Sitao; Wu, Guogan; Wu, Huawei

doi:10.1007/s00217-022-04047-2

Cited by 4 publications

(3 citation statements)

References 70 publications

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“…In a study with Anaeromyces sp, xylanase activity was investigated at pH and different temperature ranges. In the study, the best results were obtained at a maximum pH of 6 and a temperature of 40 o C [24]. Similar results were obtained in our study.…”

Section: Resultssupporting

confidence: 91%

Determination of In-vitro Optimum Conditions of Xylanase Enzyme Activity Produced by Orpinomyces sp. and Neocallimastix sp.

YÜCEL

Koçer²

2022

JPRI

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This study was aimed to determine the in-vitro optimum conditions of xylanase enzyme activity produced by Orpinomyces sp. and Neocallimastix sp. In the study, xylanase enzyme activity was investigated in terms of extracellular and intracellular total activity (TA) and specific activity (SA) levels at different time (day), pH and temperature levels. Orpinomyces sp. and Neocallimastix sp. when different days (time) were considered in terms of xylanase enzyme activity of fungi species, it was determined that there was a statistically significant positive correlation between TA=0.732 and SA=0.546 (p<0.01). It was determined that there was a statistically positive and significant relationship between TA=0.622 and SA= 0.520 at different pH levels. This situation differs in terms of temperature levels. It was determined that there was a statistically negative significant relationship between genders and SA=-0.354 (p<0.05). In this study, it is thought to contribute by determining the optimum conditions in in-vitro and industrial uses.

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Section: Resultssupporting

confidence: 91%

Determination of In-vitro Optimum Conditions of Xylanase Enzyme Activity Produced by Orpinomyces sp. and Neocallimastix sp.

YÜCEL

Koçer²

2022

JPRI

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“…As previously stated, these fungi's xylanases are active between pH 4.5 and 6.5 at 40–60°C [55]. New GH10 xylanase produced by Anaeromyces robustus was discovered in a study.…”

Section: Discussionmentioning

confidence: 88%

“…The sequence alignment of this enzyme revealed that β‐xylanase from Neocallimastix californiae has 60.22% identity (GenBank: ORY30865.1). While the pH‐optimum value of this enzyme, which was purified in the same study, was 5.5, it retained more than 90.0% of the enzyme activity and was shown to be acid xylanase [55]. It has also been discovered to have a broad pH stability range.…”

Section: Discussionmentioning

confidence: 99%

Isolation, morphological identification, and xylanase characteristics of anaerobic gut fungi Neocallimastix from Anatolian wild goat

Kar

Torcan

2022

J Basic Microbiol

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This study shows the morphological identification of anaerobic fungal strains isolated from fecal samples of goats inhabiting Turkey and the effects of various metal ions and chemicals on extracellular xylanase production. Three different anaerobic gut fungi isolated from wild goats in Turkey were identified as Neocallimastix spp. xylanase, cellulase, and lichenase production were tested in culture supernatants, and the maximum-specific activities were found as 560.42 ± 9.39, 159.70 ± 3.88, and 157.36 ± 3.83 (μmol/min/mg protein), respectively. While the optimum temperature range of exoxylanases was found as 40-50°C, their optimum pH range was determined as 6.0-6.5. Xylanase activity decreased in metal ions and other chemical reactants based on dose. The metal ion that significantly inhibited xylanase activity was Fe +3 . It was found that the ferric ions inhibited xylanase activity in all three anaerobic gut fungi by 30%-90% depending on molarity. On

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Microbiome engineering and ruminants

Zhang,

Jin,

Liu

et al. 2025

Human and Animal Microbiome Engineering

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Heterologous expression and characterization of Anaeromyces robustus xylanase and its use in bread making

Cited by 4 publications

References 70 publications

Determination of In-vitro Optimum Conditions of Xylanase Enzyme Activity Produced by Orpinomyces sp. and Neocallimastix sp.

Determination of In-vitro Optimum Conditions of Xylanase Enzyme Activity Produced by Orpinomyces sp. and Neocallimastix sp.

Isolation, morphological identification, and xylanase characteristics of anaerobic gut fungi Neocallimastix from Anatolian wild goat

Microbiome engineering and ruminants

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